[Federal Register: January 5, 2010 (Volume 75, Number 2)] [Proposed Rules] [Page 605-649] From the Federal Register Online via GPO Access [wais.access.gpo.gov] [DOCID:fr05ja10-26] [[Page 605]] ----------------------------------------------------------------------- Part IV Department of the Interior ----------------------------------------------------------------------- Fish and Wildlife Service ----------------------------------------------------------------------- 50 CFR Part 17 Endangered and Threatened Wildlife and Plants; Listing Foreign Bird Species in Peru and Bolivia as Endangered Throughout Their Range; Proposed Rule [[Page 606]] ----------------------------------------------------------------------- DEPARTMENT OF THE INTERIOR Fish and Wildlife Service 50 CFR Part 17 [Docket No. FWS-R9-IA-2009-0059] [96100-1671-0000-B6] [RIN 1018-AV77] Endangered and Threatened Wildlife and Plants; Listing Foreign Bird Species in Peru and Bolivia as Endangered Throughout Their Range AGENCY: Fish and Wildlife Service, Interior. ACTION: Proposed rule. ----------------------------------------------------------------------- SUMMARY: We, the U.S. Fish and Wildlife Service (Service), propose to list the following six South American bird species (collectively referred to as ``species'' for purposes of this proposed rule) as endangered under the Endangered Species Act of 1973, as amended (Act) (16 U.S.C. 1531 et seq.): ash-breasted tit-tyrant (Anairetes alpinus), Junin grebe (Podiceps taczanowskii), Junin rail (Laterallus tuerosi), Peruvian plantcutter (Phytotoma raimondii), royal cinclodes (Cinclodes aricomae), and white-browed tit-spinetail (Leptasthenura xenothorax)-- all native to Peru. The ash-breasted tit-tyrant and royal cinclodes are also native to Bolivia. This proposal, if made final, would extend the Act's protection to these species. The Service seeks data and comments from the public on this proposed rule. DATES: We will accept comments received or postmarked on or before March 8, 2010. We must receive requests for public hearings, in writing, at the address shown in the FOR FURTHER INFORMATION CONTACT section by February 19, 2010. ADDRESSES: You may submit comments by one of the following methods:Federal eRulemaking Portal: http://www.regulations.gov. Follow the instructions for submitting comments on Docket No. FWS-R9-IA-2009- 0059. U.S. mail or hand-delivery: Public Comments Processing, Attn: FWS-R9-IA-2009-0059; Division of Policy and Directives Management; U.S. Fish and Wildlife Service; 4401 N. Fairfax Drive, Suite 222; Arlington, VA 22203. We will not accept comments by e-mail or fax. We will post all comments on http://www.regulations.gov. This generally means that we will post any personal information you provide us (see the Public Comments section below for more information). FOR FURTHER INFORMATION CONTACT: Douglas Krofta, Chief, Branch of Listing, Endangered Species Program, U.S. Fish and Wildlife Service, 4401 N. Fairfax Drive, Room 420, Arlington, VA 22203; telephone 703- 358-2105; facsimile 703-358-1735. If you use a telecommunications device for the deaf (TDD), call the Federal Information Relay Service (FIRS) at 800-877-8339. SUPPLEMENTARY INFORMATION: Public Comments We intend that any final action resulting from this proposal will be as accurate and as effective as possible. Therefore, we request comments or suggestions on this proposed rule. We particularly seek comments concerning: (1) Biological, commercial trade, or other relevant data concerning any threats (or lack thereof) to these species and regulations that may be addressing those threats. (2) Additional information concerning the taxonomy, range, distribution, and population size of these species, including the locations of any additional populations of these species. (3) Additional information on the biological or ecological requirements of these species. (4) Current or planned activities in the areas occupied by these species and possible impacts of such activities on these species. (5) Any information concerning the effects of climate change on these species or their habitats. You may submit your comments and materials concerning this proposed rule by one of the methods listed in the ADDRESSES section. We will not consider comments sent by e-mail or fax or to an address not listed in the ADDRESSES section. If you submit a comment via http://www.regulations.gov, your entire comment--including any personal identifying information--will be posted on the website. If you submit a hardcopy comment that includes personal identifying information, you may request at the top of your document that we withhold this information from public review. However, we cannot guarantee that we will be able to do so. We will post all hardcopy comments on http://www.regulations.gov. Comments and materials we receive, as well as supporting documentation we used in preparing this proposed rule, will be available for public inspection at http://www.regulations.gov, or by appointment, during normal business hours, at the U.S. Fish and Wildlife Service, Endangered Species Program, 4401 N. Fairfax Drive, Room 420, Arlington, VA 22203; telephone 703-358-2171. Background Section 4(b)(3)(A) of the Act requires us to make a finding (known as a ``90-day finding'') on whether a petition to add a species to, remove a species from, or reclassify a species on the Federal Lists of Endangered and Threatened Wildlife and Plants has presented substantial information indicating that the requested action may be warranted. To the maximum extent practicable, we make the finding within 90 days following receipt of the petition and publish our finding promptly in the Federal Register. If we find that the petition has presented substantial information indicating that the requested action may be warranted (a positive finding), section 4(b)(3)(A) of the Act requires us to commence a status review of the species if one has not already been initiated under our internal candidate assessment process. In addition, section 4(b)(3)(B) of the Act requires us to make a finding within 12 months following receipt of the petition (``12-month finding'') on whether the requested action is warranted, not warranted, or warranted but precluded by higher priority listing actions. Section 4(b)(3)(C) of the Act requires that a finding of warranted but precluded for petitioned species should be treated as having been resubmitted on the date of the warranted but precluded finding, and is, therefore, subject to a new finding within 1 year and subsequently thereafter until we publish a proposal to list or a finding that the petitioned action is not warranted. The Service publishes an annual notice of review (ANOR) of findings on resubmitted petitions for all foreign species for which listings were previously found to be warranted but precluded. Previous Federal Actions On May 6, 1991, we received a petition (the 1991 petition) from the International Council for Bird Preservation (ICBP) to add 53 foreign bird species to the List of Endangered and Threatened Wildlife, including the six Peruvian bird species that are the subject of this proposed rule. In response to the 1991 petition, we published a substantial 90-day finding on December 16, 1991 (56 FR 65207), for all 53 species and initiated a status review. On March 28, 1994 (59 FR 14496), we published a 12-month finding on the 1991 petition, along with a proposed rule to list 30 African birds under the Act (which included 15 species from the 1991 petition). In that [[Page 607]] document, we announced our finding that listing the remaining 38 species from the 1991 petition, including the six Peruvian bird species that are the subject of this proposed rule, was warranted but precluded by higher priority listing actions. We made a subsequent warranted-but- precluded finding for all outstanding foreign species from the 1991 petition, including the six Peruvian bird species that are the subject of this proposed rule, as published in our ANOR on May 21, 2004 (69 FR 29354). Per the Service's listing priority guidelines (September 21, 1983; 48 FR 43098), our 2007 ANOR identified the listing priority numbers (LPNs) (ranging from 1 to 12) for all outstanding foreign species. The six Peruvian bird species that are the subject of this proposed rule were designated with an LPN of 2, and it was determined that their listing continued to be warranted but precluded because of other listing actions. A listing priority of 2 indicates that the subject species face imminent threats of high magnitude. With the exception of the listing priority ranking of 1, which addresses monotypic genera that face imminent threats of high magnitude, categories 2 and 3 represent the Service's highest priorities. On July 29, 2008 (73 FR 44062), we published in the Federal Register a notice announcing our annual petition findings for foreign species. In that notice, we announced listing to be warranted for 30 foreign bird species, including the six Peruvian bird species which are the subject of this proposed rule, and stated that we would ``promptly publish proposals to list these 30 taxa.'' In selecting these six species from the list of warranted-but-precluded species, we took into consideration the magnitude and immediacy of the threats to the species, consistent with the Service's listing priority guidelines. On September 8, 2008, the Service received a 60-day notice of intent to sue from the Center for Biological Diversity (CBD) and Peter Galvin over violations of section 4 of the Act for the Service's failure to promptly publish listing proposals for the 30 ``warranted'' species identified in our 2008 ANOR. Under a settlement agreement approved by the U.S. District Court for the Northern District of California on June 15, 2009, (CBD, et al. v. Salazar, 09-cv-02578-CRB), the Service must submit to the Federal Register proposed listing rules for the ash-breasted tit-tyrant, Junin grebe, Junin rail, Peruvian plantcutter, royal cinclodes, and white-browed tit-spinetail by December 29, 2009. Species Information and Factors Affecting the Species Section 4 of the Act (16 U.S.C. 1533), and its implementing regulations at 50 CFR part 424, set forth the procedures for adding species to the Federal Lists of Endangered and Threatened Wildlife and Plants. A species may be determined to be an endangered or threatened species due to one or more of the five factors described in section 4(a)(1) of the Act. The five factors are: (A) The present or threatened destruction, modification, or curtailment of its habitat or range; (B) overutilization for commercial, recreational, scientific, or educational purposes; (C) disease or predation; (D) the inadequacy of existing regulatory mechanisms; and (E) other natural or manmade factors affecting its continued existence. Listing actions may be warranted based on any of the above threat factors, singly or in combination. Below is a species-by-species analysis of the five factors. The species are considered in alphabetical order, beginning with the ash- breasted tit-tyrant, followed by the Junin grebe, Junin rail, Peruvian plantcutter, royal cinclodes, and the white-browed tit-spinetail. I. Ash-breasted tit-tyrant (Anairetes alpinus) Species Description The ash-breasted tit-tyrant, locally known as ``torito pechicenizo,'' is a small New World tyrant flycatcher in the Tyrannidae family that is native to high-altitude woodlands of the Bolivian and Peruvian Andes (BirdLife International (BLI) 2000, p. 392; Collar et al. 1992, p. 753; del Hoyo et al. 2004, pp. 170, 281; Fjeldsa and Krabbe 1990, pp. 468-469; InfoNatura 2007, p. 1; Supreme Decree No. 034-2004-AG 2004, p. 276854). The sexes are similar, with adults approximately 5 inches (in) (13 centimeters (cm)) in length, with dark gray, inconspicuously black-streaked upperparts (BLI 2009o, p. 1; del Hoyo et al. 2004, p. 281). Two subspecies (see Taxonomy) are distinguished by their underbelly color, which is yellowish-white in the nominate subspecies and white in the other (BLI 2009o, p. 1) (see Taxonomy). Juvenile plumage is duller in appearance, but otherwise similar to the adult coloration (del Hoyo et al. 2004, p. 281). Taxonomy When the species was first taxonomically described by Carriker (1933, pp. 27-29), it was placed in its own genus, Yanacea; this decision was soon questioned by Zimmer (1940, p. 10). It was not until the 1960s that Yanacea was merged into Anairetes (a genus long-known as Spizitornis) by Meyer de Schauensee (1966, p. 376). Some contemporary researchers have suggested retaining the species within Yanacea (Fjeldsa and Krabbe 1990, p. 468). Smith (1971, pp. 269, 275) and Roy et al. (1999, p. 74) confirmed that the ash-breasted tit tyrant is a valid species based on its phylogenetic placement and degree of genetic divergence from other species of Anairetes, and recent texts continue to place it in Anairetes (e.g., del Hoyo et al. 2004, p. 281). Therefore, we accept the species as Anairetes alpinus, which also follows the Integrated Taxonomic Information System (ITIS 2009, p. 1). Two subspecies are recognized, including, A. alpinus alpinus (the nominate subspecies) and A. alpinus bolivianus. These subspecies occur in two disjunct (widely separated) areas (see Current Range) (BLI 2000, p. 392; del Hoyo et al. 2004, p. 281; ITIS 2009, p. 1) and are distinguished by the color of their underbellies (see Taxonomy) (BLI 2009o, p. 1). Habitat and Life History The ash-breasted tit-tyrant is restricted to semihumid Polylepis or Polylepis - Gynoxys woodlands, where the species is found at elevations between 12,139 and 15,092 feet (ft) (3,700 and 4,600 meters (m)) above sea level (BLI 2000, p. 392; Collar et al. 1992, p. 753; del Hoyo et al. 2004, p. 281; Fjeldsa and Krabbe 1990, pp. 468-469; InfoNatura 2007, p. 1). The genus Polylepis (no English common name; locally referred to as ``queu[ntilde]a'') (Aucca and Ramsay 2005, p. 1), in the Rosaceae family, comprises approximately 20 species of evergreen bushes and trees (De la Via 2004, p. 10; Kessler 1998, p. 1; Kessler and Schmidt-Lebuhn 2006, pp. 1-2), 19 of which occur in Peru (Chutas et al. 2008, p. 3). In Bolivia, the ash-breasted tit-tyrant is associated only with P. pepei forests, but the bird is found among a greater variety of Polylepis species in Peru (Chutas et al. 2008, p. 16; I. Gomez, National Museum of National History-Ornithology Section-Bolivian Fauna Collection, La Paz, Bolivia, e-mail to Division of Scientific Authority, in litt. December 4, 2007, p. 1). On average, Polylepis species are 10-33 ft (3-10 m) tall, but may grow to a height of 118 ft (36 m) (Purcell et al. 2004, p. 455). Polylepis woodlands occur as dense forests, as open-canopied stands with more arid understories, or as shrubland with scattered trees (De la Via 2004, pp. 10-11; Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113; Lloyd and [[Page 608]] Marsden in press, as cited in Lloyd 2008, p. 532). Ash-breasted tit- tyrants prefer dense Polylepis forests (Fjeldsa 2002a, p. 114; Smith 1971, p. 269), which often include a mixture of Gynoxys trees (no common name), in the Asteraceae family (De la Via 2004, pp. 10; International Plant Names Index (IPNI) 2009, p. 1). Dense Polylepis woodlands are characterized by moss- or vine-laden vegetation, with a shaded understory and a rich diversity of insects, making good feeding grounds for insectivorous birds (De la Via 2004, p. 10), such as the ash-breasted tit-tyrant (BLI 2009o, p. 1; Lloyd 2008, p. 535). There is little information about the ecology and breeding behavior of the ash-breasted tit-tyrant. The species' territory ranges from 2.5- 5 acres (ac) (1-2 hectares (ha)) (BLI 2009o, p. 1). The breeding season appears to occur during late dry season (Collar et al. 1992, p. 754)-- November and December (BLI 2009o, p. 1). Juveniles have been observed in March and July (Collar et al. 1992, p. 754; del Hoyo et al. 2004, p. 281). Although species-specific information is not available, tit- tyrant nests are generally finely woven, open cups, built in a bush (Fjeldsa and Krabbe 1990, p. 468). The species may share in rearing responsibilities, as pairs of ash-breasted tit-tyrants have been observed feeding young (BLI 2009o, p. 1; Collar et al. 1992, p. 754). The ash-breasted tit-tyrant forages alone, in family groups, and sometimes in mixed-species flocks. The bird takes short flights, either hovering or perching to consume invertebrates near the tops and outer edges of Polylepis shrubs and trees (BLI 2009o, p. 1; del Hoyo et al. 2004, p. 281; Engblom et al. 2002, p. 58; Fjeldsa and Krabbe 1990, p. 468; Lloyd 2008, p. 535). In winter, when invertebrate populations diminish, tit-tyrants may also forage on seeds (Fjeldsa and Krabbe 1990, p. 468). Historical Range and Distribution The ash-breasted tit-tyrant may once have been well-distributed throughout previously dense and contiguous Polylepis high-Andes woodlands of Peru and Bolivia. Researchers believe that these woodlands were historically contiguous with lower-elevation cloudforests and widespread above 9,843 ft (3,000 m) (Collar et al. 1992, p. 753; Fjeldsa 2002a, pp. 111-112, 115; Herzog et al. 2002, p. 94; Kessler 2002, pp. 97-101). Today, Polylepis woodlands are found only at elevations of 11,483 to 16,404 ft (3,500 to 5,000 m) (Fjeldsa 1992, p. 10). Researchers consider the reduction in Polylepis forest habitat to be the result of historical human activities, including burning and grazing, which have prevented regeneration of the woodlands and resulted in the fragmented habitat distribution seen today (Fjeldsa and Kessler 1996, Kessler 1995a, Kessler 1995b, and L[aelig]gaard 1992, as cited in Fjeldsa 2002a, p. 112; Herzog et al. 2002, p. 94; Kessler 2002, pp. 97-101; Kessler and Herzog 1998, pp. 50-51). Modeling studies by Fjeldsa (2002a, p. 116) indicate that this habitat reduction was accompanied by a loss in species richness. It is estimated that only 2- 3 and 10 percent of the original forest cover still remain in Peru and Bolivia, respectively (Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113). Of this amount, only 1 percent of the remaining Polylepis woodlands are found in humid areas, where denser stands occur (Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113) and which are preferred by the ash-breasted tit-tyrant (BLI 2009o, p. 1; Fjeldsa 2002a, p. 114; Lloyd 2008, p. 535; Smith 1971, p. 269) (see Factor A). Current Range and Distribution The current range of the ash-breasted tit-tyrant is estimated to be 4,595 square miles (mi\2\) (11,900 square kilometers (km\2\)) (BLI 2009o, p. 1). However, BirdLife International (2000, pp. 22, 27) defines a species' ``Range'' as the ``Extent of Occurrence,'' which is ``the area contained within the shortest continuous imaginary boundary which can be drawn to encompass all the known, inferred, or projected sites of present occurrence of a species, excluding cases of vagrancy.'' Given that the species is known to occur in two disjunct locations, this range estimate, therefore, includes a large area of habitat where the species is not known to occur. The species is restricted to patches of high-elevation semihumid Polylepis or Polylepis - Gynoxys woodlands of Peru and Bolivia, where ash-breasted tit-tyrant is found only at 12,139-15,092 ft (3,700-4,600 m) (BLI 2000, p. 392; Collar et al. 1992, p. 753; del Hoyo et al. 2004, pp. 170, 281; Fjeldsa and Krabbe 1990, pp. 468-469; InfoNatura 2007, p. 1). The ash-breasted tit-tyrant is known only in two disjunct areas: one location in west-central Peru and another ranging from southern Peru into northern Bolivia (BLI 2000, p. 392; del Hoyo et al. 2004, p. 281; ITIS 2009, p. 1). In west-central Peru, the northern subspecies (A. alpinus alpinus) occurs in the Cordilleras Central and Occidental (in the Peruvian Administrative Regions of Ancash, Huanuco, La Libertad, and Lima) (BLI 2007, p. 1; BLI 2009g, p. 1; BLI 2009i, p. 1; BLI 2009l, p. 1; BLI 2009o, p. 1; Collar et al. 1992, p. 753; del Hoyo et al. 2004, p. 281). Until 1992, the taxon in this locality was highly localized and known only in Ancash Region (Collar et al. 1992, p. 753). The species was subsequently reported in Huanuco Region, in 2003 (BLI 2007, p. 5; BLI 2009i, p. 1); La Libertad Region, in 2004 (del Hoyo et al. 2004, p. 281); and Lima Region and again in Huanuco Region, in 2007 (BLI 2007, pp. 1, 5). Also in 2007, the ash-breasted tit-tyrant was also observed in a new locality in Ancash Region, Corredor Conchucos (Chutas 2007, pp. 4, 8), where a Polylepis reforestation project is under way to connect two protected areas where ash-breasted tit-tyrants were already known to occur, Parque Nacional Huascaran and Zona Reservada de la Cordillera Huayhuash (Antamina Mine 2006, p. 5; MacLennan 2009, p. 1) (see Factor A). The second location spans the Peruvian-Bolivian border--in the Peruvian Administrative Regions of Apurimac, Cusco, Puno, and Arequipa (from north to south) and in the Bolivian Department of La Paz. Here, the southern subspecies (A. alpinus bolivianus) occurs in Cordillera Oriental (Apurimac and Cusco), Cordilleras Vilcanota and Vilcabamba (Cusco), and Cordillera de Carabaya (Puno)--in Peru--and ranges into Bolivia, where it is found in the Cordillera Real and the Cordillera Apolobamba (La Paz) (BLI 2000, p. 392; BLI 2009a, p. 1; BLI 2009b, p. 1; BLI 2009c, p. 1; BLI 2009d, p. 1; BLI 2009e, p. 1; BLI 2009f, p. 1; BLI 2009k, p. 1; BLI 2009m, p. 1; BLI 2009n, p. 1; BLI 2009o, p. 5; Chutas 2007, p. 8; Collar et al. 1992, p. 753; del Hoyo et al. 2004, p. 281; Fjeldsa and Krabbe 1990, pp. 468-469; InfoNatura 2007, p. 1). The ash-breasted tit-tyrant was only recently (in 2008) reported in Arequipa Region, Peru (BLI 2009j, p. 1). The ash-breasted tit-tyrant is highly localized (Collar et al. 1992, p. 753) and has been described as ``very rare and local, with usually only 1-2 pairs per occupied woodland'' (Fjeldsa and Krabbe 1990, p. 469). It exists at such low densities in some places that it goes undetected (Collar et al. 1992, p. 753). The species appears to be unable to persist in forest remnants smaller than 2.5 ac (1 ha) (BLI 2009o, p. 1). Population Estimates Population information is presented first on the range country level and then in terms of a global population estimate. The range country estimates begin with Peru, where the majority of the population resides. Local population estimate, Peru: Chutas (2007, p. 8) surveyed five [[Page 609]] disjunct Polylepis forest patches in Peru and estimated that 461 ash- breasted tit-tyrants were located there. This included 30 birds in Corredor Conchucos (Ancash Region); 181 birds and 33 birds in Cordilleras Vilcanota and Vilcabamba, respectively (Cusco Region); 22 birds in Cordillera de Carabaya (Puno Region); and 195 birds in a study site called ``Cordillera del Apurimac'' (Apurimac Region) (Chutas 2007, pp. 4, 8), referring to an area within the Runtacocha highlands. Other research in the Runtacocha highlands has indicated that the ash- breasted tit-tyrant is ``relatively common'' there (BLI 2007, p. 1; BLI 2009o, p. 1), with an estimated 100 pairs of birds found in approximately 40 forest patches (Fjeldsa in litt. 1990, as cited in Collar et al. 1992, p. 753). ``Small numbers'' of birds are reported in La Libertad Region (del Hoyo et al. 2004, p. 281). There are no population estimates within Arequipa, Huanuco, or Lima Regions, where the ash-breasted tit-tyrant is also reported to occur (BLI 2009g, p. 1; BLI 2009i, p. 1; BLI 2009j, p. 1; BLI 2009o, p. 1). Local population estimate, Bolivia: Although BirdLife International reports an estimated population size of 150-300 ash-breasted tit- tyrants in Bolivia (Gomez in litt., 2003 and 2007, as cited in BLI 2009o, p. 1), recent surveys indicate that the estimate is smaller. Over a six-year period, Gomez (in litt. 2007, p. 1) conducted intensive searches throughout 80 percent of the suitable habitat in Bolivia in the Cordillera Real and the Cordillera Apolobamba (La Paz Department), using song playback and point census, to detect the presence of the ash-breasted tit-tyrant. From this work, researchers inferred or observed the presence of 2-10 individuals in each of four forest patches and estimated that approximately 180 ash-breasted tit-tyrants occur in Bolivia. Global population estimate: BirdLife International, a global organization that consults with and assimilates information from species experts, categorizes the ash-breasted tit-tyrant as having a population size between 250 and 999 individuals, with an estimated actual population size to be in the mid- to upper-hundreds (BLI 2000, p. 392; BLI 2007, p. 1; BLI 2009o, p. 1). Combining the estimated number of ash-breasted tit-tyrants in Peru and Bolivia, the total population consists of at least 641 individuals (Chutas 2007, pp. 4, 8; Gomez in litt. 2007, p. 1), consistent with the BirdLife International category of 250-999 individuals. However, the total population size, which includes immature individuals, is not an accurate reflection of the species' effective population size (the number of breeding individuals that contribute to the next generation) (Shaffer 1981, pp. 132-133; Soule 1980, pp. 160- 162). Because the ash-breasted tit-tyrant exists as two subspecies (BLI 2000, p. 392; BLI 2009a, p. 1; BLI 2009b, p. 1; BLI 2009c, p. 1; BLI 2009d, p. 1; BLI 2009e, p. 1; BLI 2009f, p. 1; BLI 2009k, p. 1; BLI 2009m, p. 1; BLI 2009n, p. 1; BLI 2009o, p. 5; Chutas 2007, p. 8; Collar et al. 1992, p. 753; del Hoyo et al. 2004, p. 281; Fjeldsa and Krabbe 1990, pp. 468-469; InfoNatura 2007, p. 1; ITIS 2009, p. 1), it is reasonable to conclude that there is little or no gene flow between the two subspecies and that the species does not breed as a single population. Therefore, even if all 641 individuals were mature, they would not breed as a single population, such that the species' effective population size is less than 641. There are also constraints to determining the effective population size on a subspecies level. According to International Union for Conservation of Nature (IUCN) criteria, it is estimated that there are no more than 250 mature individuals in any single subpopulation of the ash-breasted tit-tyrant (IUCN 2001, pp. 8-12). However, the parameters of a subpopulation are not provided in existing research. For instance, while ash-breasted tit-tyrants occupy territories of 2.5-5 ac (1-2 ha) (BLI 2009o, p. 1), there is no information as to the taxon's ability or tendency to travel between territories or forest patches. Research on Bolivian localities indicates that gene flow occurs between some subpopulations, but not all (Gomez 2005, p. 86). In Bolivia, where only 1 subspecies occurs, the birds are distributed in 2 metapopulations, with at least 5 subpopulations in one location and 14 subpopulations in the other (Gomez 2005, p. 86). Peruvian population estimates are incomplete, with no estimates for the ash-breasted tit-tyrants occurring in Arequipa, Huanuco, La Libertad or Lima (BLI 2009g, p. 1; BLI 2009i, p. 1; BLI 2009j, p. 1; BLI 2009o, p. 1; del Hoyo et al. 2004, p. 281). Therefore, we can conclude that gene flow occurs at the subspecies level, but there is not sufficient information to determine the extent to which subpopulations interbreed. The species has experienced a population decline of between 10 and 19 percent in the past 10 years, and this rate of decline is predicted to continue (BLI 2009o, pp. 1, 4). The population is considered to be declining in close association with continued habitat loss and degradation (see Factors A and E) (BLI 2007, pp. 1, 4; BLI 2009o, p. 5). Conservation Status The ash-breasted tit-tyrant is considered ``endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276855). The IUCN considers the ash-breasted tit-tyrant to be ``Endangered'' because it has a very small population that is undergoing continued decline in the number of mature individuals and is confined to a habitat that is severely fragmented and is also undergoing a continuing decline in extent, area, and quality (BLI 2008, p. 1; BLI 2009o, p. 4; IUCN 2001, pp. 8-12). The ash-breasted tit- tyrant occurs within the following Peruvian protected areas: Parque Nacional Huascaran, in Ancash, and Santuario Historico Machu Picchu, in Cusco, and Zona Reservada de la Cordillera Huayhuash, spanning Ancash, Huanuco, and Lima (BLI 2009i, p. 1; BLI 2009l, p. 1; BLI 2009n, p. 1; Chutas et al. 2008, p. 16). In La Paz Department, Bolivia, the species is found in Parque Nacional y area Natural de Manejo Integrado Madidi, Parque Nacional y area Natural de Manejo Integrado Cotapata, and the co-located protected areas of Reserva Nacional de Fauna de Apolobamba, area Natural de Manejo Integrado de Apolobamba, and Reserva de la Biosfera de Apolobamba (Auza and Hennessey 2005, p. 81; BLI 2009a, p. 1; BLI 2009b, p. 1; BLI 2009c, p. 1; BLI 2009e, p. 1; BLI 2009i, p. 1; Chutas et al. 2008, p. 16). Summary of Factors Affecting the Ash-breasted Tit-tyrant A. The Present or Threatened Destruction, Modification, or Curtailment of the Species' Habitat or Range The ash-breasted tit-tyrant is dependent upon high-elevation semihumid Polylepis or Polylepis - Gynoxys woodlands (BLI 2000, p. 392; Collar et al. 1992, p. 753; del Hoyo et al. 2004, pp. 281; Fjeldsa and Krabbe 1990, pp. 468-469; InfoNatura 2007, p. 1). Researchers believe that this habitat was historically contiguous with lower-elevation cloudforests and widespread above 9,843 ft (3,000 m) (Collar et al. 1992, p. 753; Fjeldsa 2002a, pp. 111, 115), but Polylepis woodlands occur today only between 11,483-16,404 ft (3,500-5,000 m) (Fjeldsa 1992, p. 10). As described above (see Habitat and Life History), the species prefers dense woodlands (Fjeldsa 2002a, p. 114; Smith 1971, p. 269), where the best foraging habitat occurs (De la Via 2004, p. 10), and ash-breasted tit-tyrant occurs at altitudes of 12,139-15,092 ft (3,700-4,600 m) (BLI 2000, p. 392; Collar et al. 1992, p. 753; del Hoyo et al. 2004, pp. [[Page 610]] 170, 281; Fjeldsa and Krabbe 1990, pp. 468-469; InfoNatura 2007, p. 1). High-Andean Polylepis woodlands are considered by experts to be the most threatened habitat in Peru and Bolivia (Purcell et al. 2004, p. 457), throughout the Andean region (BLI 2009a, p. 2), and one of the most threatened woodland ecosystems in the world (Renison et al. 2005, as cited in Lloyd 2009, p. 10). The IUCN has listed several Polylepis species as ``Vulnerable,'' including two species that occur within the ash-breasted tit-tyrant's range, Polylepis incana and P. pepei (WCMC 1998a, p. 1; WCMC 1998b, p. 1). Peruvian and Bolivian Polylepis woodlands today are highly fragmented. In the late 1990s, Fjeldsa and Kessler (1996, as cited in Fjeldsa 2002a, p. 113) conducted comprehensive ground surveys and analyzed maps and satellite images of the area. They estimated that the current range of Polylepis woodlands had been reduced from historical levels by 97-98% in Peru and 90 percent in Bolivia. Contemporary Polylepis woodlands are dispersed and sparse, covering an estimated area of 386 mi\2\ (1,000 km\2\) and 1,931 mi\2\ (5,000 km\2\) in Peru and Bolivia, respectively (Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113). Of the remaining Polylepis woodlands, only 1 percent are found in humid areas, where denser Polylepis forests tend to occur (Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113). Dense habitat is preferred by the ash- breasted tit-tyrant (BLI 2009o, p. 1; Fjeldsa 2002a, p. 114; Lloyd 2008, p. 535; Smith 1971, p. 269). Habitat loss, conversion, and degradation throughout the ash- breasted tit-tyrant's range have been and continue to occur as a result of ongoing human activity, including (1) Clearcutting and burning; (2) extractive activities; (3) human encroachment; and (4) unpredictable climate fluctuations that exacerbate the effects of habitat fragmentation. Clearcutting and burning are among the most destructive activities, and are a leading cause for Polylepis habitat loss (WCMC 1998a, p. 1; WCMC 1998b, p. 1). Forested areas are cleared for agriculture and to create pastureland for cattle, sheep, and camels (BLI 2009a, p. 2; BLI 2009c, pp. 1-2; BLI 2009d, pp. 1-2; BLI 2009e, pp. 1, 5; BLI 2009h, p. 1; BLI 2009m, p. 1; BLI 2009n, p. 4). Grazing lands situated amongst remaining forest patches are regularly burned in order to maintain the grassland vegetation (locally known as, ``chaqueo''). Regular burning prevents regeneration of native forests and is considered the key factor limiting the distribution of Polylepis forests (BLI 2009f, p. 1; BLI 2009n, p. 4; Fjeldsa 2002b, p. 8; WCMC 1998a, p. 1; WCMC 1998b, p. 1). In some areas, the burns escape control, causing further habitat destruction (BLI 2009a, p. 2; BLI 2009e, pp. 1, 5). Burning and clearcutting occur throughout the ash- breasted tit-tyrant's range, including in Ancash (BLI 2009h, p. 1), Apurimac (BLI 2009m, p. 1), and Cusco (BLI 2009n, p. 4), in Peru; and La Paz, Bolivia (BLI 2009a, p. 2; BLI 2009c, pp. 1-2; BLI 2009d, pp. 1- 2; BLI 2009e, pp. 1, 5). These activities are also ongoing within protected areas, including Parque Nacional Huascaran, Santuario Historico Machu Picchu, and Zona Reservada de la Cordillera Huayhuash (Barrio 2005, p. 564; BLI 2009l, p. 4; BLI 2009n, p. 2) (see Factor D). As a result of the intensity of burning and grazing, Polylepis species are restricted to areas where fires cannot spread, and where cattle and sheep do not normally roam--in stream ravines and on boulders, rock ledges, and sandy ridges (Fjeldsa 2002a, p. 112; Fjeldsa 2002b, p. 8). Grazing and trampling by domesticated animals further limit forest regeneration (Fjeldsa 2002a, p. 120) and contribute to the degradation of remaining forest patches. Sheep and cattle have solid, sharp hooves that churn up the earth, damaging vegetation and triggering erosion (Engblom et al. 2002, p. 56; Purcell et al. 2004, p. 458). The loss of nutrient-rich soils leads to habitat degradation, which reduces the ability of the habitat to support dense stands of Polylepis woodlands (Fjeldsa 2002b, p. 8; Jameson and Ramsay 2007, p. 42; Purcell et al. 2004, p. 458). Ash-breasted tit-tyrant habitat is also subject to conversion, degradation, or destruction caused by extractive activities, such as firewood collection, timber harvest, and mining. Cutting wood for fuel has a consistent and ongoing impact throughout the species' range (BLI 2009a, p. 2; BLI 2009b, pp. 1-2; BLI 2009c, pp. 1-2; BLI 2009d, pp. 1- 2; BLI 2009f, p. 1; BLI 2009l, p. 1; WCMC 1998a, p. 1). The high- altitude zones where Polylepis occurs have long been inhabited by subsistence farmers, who rely on Polylepis wood for firewood and charcoal production (Aucca and Ramsay 2005, p. 287). Many locals manage woodlands for firewood extraction (Engblom et al. 2002, p. 56), and community-based Polylepis conservation programs fostered by the Peruvian nongovernmental organization Asociacion Ecosistemas Andinos (ECOAN) have been under way in Peru and Bolivia since 2004, encompassing Cordilleras Vilcanota and Vilcabamba (Cusco Region), highlands of the Apurimac Region (Aucca and Ramsey 2005, p. 287; ECOAN no date (n.d.), p. 1; Lloyd 2009, p. 10), and in the Ancash Region (MacLennan 2009, p. 2). Known as the ``Vilcanota Project'' or ECOAN Projects (Aucca and Ramsey 2005, p. 287; ECOAN n.d., p. 1), local communities enter into and enforce management agreements aimed at the primary causes for Polylepis deforestation: burning, grazing, and wood- cutting. These projects foster local, sustainable use of resources (Aucca and Ramsay 2005, p. 287; ECOAN n.d., p. 1; Engblom et al. 2002, p. 56), such as the use of more fuel-efficient wood-burning stoves that require half the amount of wood fuel (MacLennan 2009, p. 2) (see the Factor A analyses for royal cinclodes and white-browed tit-spinetail for additional examples). Polylepis wood is also harvested for local commercial use, including within protected areas (BLI 2009a, p. 2; WCMC 1998a, p. 1) (Factor D). At one site, near Abra Malaga (Cusco Region), wood is harvested for sale to local hotels in the towns of Urubamba and Ollantaytambo to support tourism activity (Engblom 2000, p. 1). Engblom (2000, p. 1) documented felling for firewood at this site in Cusco over a 2-day period that significantly reduced the size and quality of the forest patch. Purcell et al. (2004, p. 458) noted a positive correlation between habitat destruction and increased demand for (and the concomitant rise in the price of) fuel. Polylepis is also harvested for construction, fencing, and tool-making (Aucca and Ramsey 2005, p. 287; BLI 2009a, p. 2). Commercial-scale activities, such as clearcutting, logging, tourism, and infrastructure development, are ongoing throughout this species' range, and alter otherwise sustainable resource use practices (Aucca and Ramsay 2005, p. 287; Engblom 2000, p. 2; Engblom et al. 2002, p. 56; MacLennan 2009, p. 2; Purcell and Brelsford 2004, pp. 156-157; Purcell et al. 2004, pp. 458-459; WCMC 1998a, p. 1). Commercial-scale resource use is exacerbated by ongoing human encroachment, discussed below. Mining in Polylepis habitat occurs in the Peruvian Regions of Ancash and Huanaco and in the Bolivian Department of La Paz (BLI 2009b, p. 1; BLI 2009d, p. 1; BLI 2009g, p. 1). Ancash (Peru) is home to the largest zinc and copper mine ``in the world,'' with a monthly average production rate of 231,485 pounds (105,000 metric tons) of minerals per day and a 186-mile (mi) (300 kilometer (km)) pipeline that stretches from the mine to the port of Punta Lobitos along the coast (Antamina Mine 2006, pp. 4, 9; www.antamina.com/02_operacion/ [[Page 611]] En_puerto.html). Mining occurs in ash-breast tit-tyrant habitat in La Paz, Bolivia, where there are active gold, tin, silver, and tungsten mines, in addition to gravel excavation for cement production (USGS Minerals Yearbook 2005, pp. 4-7). Antamina Mine has undertaken habitat conservation programs within the areas affected by mineral extraction, similar to the ECOAN Polylepis conservation programs, investing millions of dollars in programs ranging from education and tourism, to organic agriculture and sustainable development. However, tourism has had negative effects in other areas where the ash-breasted tit-tyrant occurs, including Ancash, Huanuco, and Lima, Peru, and La Paz, Bolivia (Barrio 2005, p. 564; BLI 2009e, p. 5) (see below). The Antamina Mining Company conservation program also supports the planned reforestation of 123,552 ac (50,000 ha) of Polylepis forest that will connect two protected areas, Parque Nacional Huascaran and Zona Reservada de la Cordillera Huayhuash (Antamina Mine 2006, p. 5). To date, the project has succeeded in restoring 371 ac (150 ha) of forest, with a 95 percent survival rate (MacLennan 2009, p. 1). Known as Corredor Conchucos, at least 30 ash-breasted tit-tyrants have recently been observed there (Chutas 2007, p. 8). Human encroachment and concomitant increasing human population pressures exacerbate the destructive effects of ongoing human activities throughout the ash-breasted tit-tyrant's habitat. Mining and hydroelectric projects open new areas to exploitation and attract people seeking employment (Purcell et al. 2004, p. 458). Increased urbanization and mining have led to increased infrastructure development. Road building and mining projects further facilitate human access to remaining Polylepis forest fragments, throughout the ash- breasted tit-tyrant's range (Purcell et al. 2004, pp. 458-459; Purcell and Brelsford, 2004, pp. 156-157), including protected areas. In the Bolivian Department of La Paz, one of the most transited highways in the country is located a short distance from the Parque Nacional y area Natural de Manejo Integrado Cotapata (BLI 2009b, p. 2) (see Factor D). Road building, mining, and other large-scale resource exploitations are considered to have major impacts on the habitat, as compared to exploitation by local communities (Purcell and Brelsford 2004, p. 157). Ecotourism within protected areas where the ash-breasted tit-tyrant occurs (such as in the Zona Reservada de la Cordillera Huayhuash in Peru, and in the Apolobamba protected areas in Bolivia) is considered a growing problem (Barrio 2005, p. 564; BLI 2009e, p. 5) (see Factor D). In the Department of La Paz, Bolivia, which encompasses Bolivia's largest urban area, most of the Polylepis forest had been eliminated prior to the late 1990s (Purcell and Brelsford 2004, p. 157). Recently, an accelerated rate of Polylepis forest destruction has been attributed to clearcutting for road building and industrialization projects, such as mining and construction of hydroelectric power stations (Purcell and Brelsford 2004, pp. 156-157). Between 1991 and 2003, approximately 494 ac (200 ha) of Polylepis habitat was destroyed. Thus, nearly two-thirds of the forest cover that existed in the 1990s no longer existed in 2003 (Purcell and Brelsford 2004, p. 155). With this research, it was estimated that only 1,285 ac (520 ha) of Polylepis forest remain in the Bolivian Department of La Paz, representing approximately a 40 percent rate of habitat loss in just over one decade. The researchers inferred that thisrate of destruction could result in extirpation of the remaining Polylepis forest in La Paz within the next 30 years (Purcell and Brelsford 2004, pp. 157). Larger concentrations of people put greater demand on the natural resources in the area. Increasing demand for firewood upsets informal and otherwise sustainable community-based forest management traditions (Purcell and Brelsford, 2004, p. 157). Increasing human populations in the high-Andes of Bolivia and Peru have also resulted in a scarcity of arable land. This has led many farmers to burn down additional patches of Polylepis forests to plant crops, even on steep hillsides not suitable for cultivation (BLI 2009b, p. 2; BLI 2009h, p. 1; Hensen 2002, p. 199). These ongoing farming practices result in the rapid loss of Polylepis forests from Bolivia to Peru. Habitat destruction is often caused by a combination of human activities that promote habitat degradation. In the Cordillera de Vilcanota (Cusco, Peru), where an estimated 181 birds are found (Chutas 2007, pp. 4, 8), the rate of habitat loss was studied by comparing forest cover between 1956 and 2005. This study revealed a rate of habitat loss averaging only 1 percent. However, remaining patches of Polylepis woodland were small, with a mean patch size of 7.4 ac (3 ha); four forest patches had disappeared completely; and no new patches were located within the study area (Jameson and Ramsay 2007, p. 42). Lloyd (2008, p. 532) studied bird foraging habits at three Polylepis woodland sites in the Cordillera Vilcanota during 2003-2005. The sites were described as highly fragmented, consisting of many small remnant patches (less than 2.5 ac (1 ha)) and scattered trees, separated from larger woodland tracts (greater than 25 ac (10 ha)) by distances of 98- 4,921 ft (30-1,500 m) (Lloyd and Marsden in press, as cited in Lloyd 2008, p. 532). Given that the species territory ranges from 2.5-5 ac (1-2 ha) and that the ash-breasted tit-tyrant appears to be unable to persist in forest remnants smaller than 2.5 ac (1 ha) (BLI 2009o, p. 1), these patch sizes have met or are approaching the lower threshold of the species' ecological requirements. Moreover, 10 percent of the remaining forest patches showed a decline in forest density over this time-period. Thus, habitat degradation also has serious impacts in Polylepis woodlands (Jameson and Ramsay 2007, p. 42), especially given the species' preference for dense woodlands (Fjeldsa 2002a, p. 114; Smith 1971, p. 269). The fact that no new Polylepis forest patches had become established between 1956 and 2005 underscores the long-term ramifications of ongoing burning, clearing, grazing, and other habitat- altering human activities that are pervasive throughout the ash- breasted tit-tyrant's range (BLI 2009f, p. 1; BLI 2009n, p. 4; Fjeldsa 2002b, p. 8; WCMC 1998a, p. 1; WCMC 1998b, p. 1). These activities are considered to be key factors both in preventing regeneration of Polylepis woodlands (Fjeldsa 2002a, p. 112, 120; Fjeldsa 2002b, p. 8) and in the historical decline of Polylepis -dependent bird species, including the ash-breasted tit-tyrant (Fjeldsa 2002a, p. 116). Researchers consider the species' population to be declining in close association with the continued habitat loss and degradation (BLI 2007, pp. 1, 4; BLI 2008, p. 1; BLI 2009o, p. 1). Therefore, further habitat loss will continue to impact the species' already small population size (see Factor E). Peru is subject to unpredictable climate fluctuations that exacerbate the effects of habitat fragmentation, such as those that are related to the El Ni[ntilde]o Southern Oscillation (ENSO). According to the U.S. Dept. of Commerce-National Oceanic and Atmospheric Administration (NOAA), the term ENSO refers to a range of variability associated with the southern trade winds in the eastern and central equatorial Pacific Ocean. El Ni[ntilde]o events are characterized by unusual warming of the ocean, while La Ni[ntilde]a events bring cooler ocean temperatures (Tropical Atmosphere Ocean (TAO) Project no date (n.d.), p. 1). Generally speaking, extreme ENSO events alter weather patterns, so that [[Page 612]] precipitation increases in normally dry areas, and decreases in normally wet areas. During an El Ni[ntilde]o event, rainfall dramatically increases, whereas a La Ni[ntilde]a event brings near- drought conditions (Holmgren et al. 2001, p. 89). If we consider that wildlife habitat is not only defined by substrates (vegetation, soil, water), but also atmospheric conditions, then changes in air temperature and moisture can effectively change a species' habitat. Climate change is characterized by variations in the earth's temperature and precipitation, causing changes in atmospheric, oceanic, and terrestrial conditions (Parmesan and Mathews 2005, p. 334). Global climate change and other periodic climatic patterns (e.g., El Ni[ntilde]o and La Ni[ntilde]a) can cause or exacerbate such negative impacts on a broad range of terrestrial ecosystems and Neotropical bird populations (England 2000, p. 86; Holmgren et al. 2001, p. 89; Plumart 2007, pp. 1-2; Timmermann 1999, p. 694). Unpredictable climate fluctuations negatively impact populations undergoing habitat fragmentation. In the face of an unpredictable climate, the risk of population decline due to habitat fragmentation is heightened. Mora et al. (2007, p. 1027) found that the combined effects of habitat fragmentation and climate change (in this case, warming) had a synergistic effect, rather than additive. In other words, the interactive effects of both climate fluctuation and habitat fragmentation led to a greater population decline than if either climate change or habitat fragmentation were acting alone on populations. Jetz et al. (2007, p. 1211) investigated the effects of climate change on 8,750 land bird species, including the ash-breasted tit-tyrant, that are exposed to ongoing manmade land cover changes (i.e., habitat loss). They determined that a narrow endemic, such as the ash-breasted tit-tyrant, is likely to suffer greater impacts from climate change, especially where projected range contractions are driven by manmade land conversion activities (Jetz et al. 2007, p. 1213). This is due to the species' already small population size, specialized habitat requirements, and heightened risk of extinction from stochastic demographic processes (see also Factor E). According to this study, by 2050, up to 18 percent of the ash-breasted tit-tyrant's current remaining range is likely to be unsuitable for this species due to climate change; and, by 2100, it is predicted that about 18 to 42 percent of the species' range is likely to be lost as a direct result of global climate change (Jetz et al. 2007, Supplementary Table 2, p. 73). In 1999, Timmermann (1999, p. 694) predicted that climate change events would increase the periodicity and severity of droughts and excessive rainfalls, such as those caused by El Ni[ntilde]o and La Ni[ntilde]a events. Evidence suggests that this is the case in Peru (Richter 2005, p. 24-25). Over the past decade, there have been four El Ni[ntilde]o events (1997-1998, 2002-2003, 2004-2005, and 2006-2007) and three La Ni[ntilde]a events (1998-2000, 2000-2001, and 2007-2008) (National Weather Service (NWS) 2009, p. 2). In Peru, the Andean highlands, and Polylepis species in particular, are strongly influenced by ENSO events (Christie et al. 2008, p. 1). Christie et al. (2008, p. 1) found that tree growth in P. tarapacana is highly influenced by ENSO events because ENSO cycles on the Peruvian Coast are strongest during the growing season (December-February). ENSO-related droughts can increase tree mortality and dramatically alter age structure within tree populations, especially where woodlands have undergone disturbance, such as fire and grazing (Villalba and Veblen 1997, pp. 121-123; Villalba and Veblen 1998, pp. 2624, 2637). With years of extremely high rainfall followed by years of extremely dry weather (Block and Richter 2007, p. 1), fire hazard is increased from the biomass accumulated during the wet period that dries and adds to the fuel load in the dry season (Block and Richter 2007, p. 1; Power et al. 2007, p. 898). Evidence suggests that the fire cycle in Peru has shortened, particularly in coastal Peru and west of the Andes (Power et al. 2007, pp. 897-898). Changes in the fire-regime can have broad ecological consequences (Block and Richter 2007, p. 1; Power et al. 2007, p. 898). In the case of the ash-breasted tit-tyrant, burning is considered to be a key factor preventing Polylepis regeneration (Fjeldsa 2002a, p. 112, 120; Fjeldsa 2002b, p. 8). Research in Ecuadorian Polylepis - Gynoxys mixed woodlands indicated a strong reduction in P. incana adult and seedling survival following a single fire. This indicates that the species does not recover well from even a single fire event (Cierjacks et al. 2007, p. 176). An accelerated fire cycle would exacerbate this situation. Activities that destroy and alter habitat are ongoing within protected areas. This is further discussed under Factor D. Summary of Factor A The ash-breasted tit-tyrant is dependent on Polylepis habitat, with a preference for dense woodlands. Polylepis habitat throughout the ash- breasted tit-tyrant's range has been and continues to be altered and destroyed as a result of human activities, including clearcutting and burning for agriculture, grazing lands, tourism, and industrialization; extractive activities, including firewood, timber, and mineral extraction; and human encroachment and concomitant increased pressure on natural resources. Researchers estimate that 1 percent of the dense woodlands preferred by the species remains, and that all remaining habitat is fragmented and degraded. The ash-breasted tit-tyrant currently occupies an area of approximately 4,595 mi\2\ (11,900 km\2\) and appears to be unable to persist in forest remnants smaller than 2.5 ac (1 ha). Forest fragments in some portions of the ash-breasted tit- tyrant's range are approaching the lower threshold of the species' ecological requirements. The historical decline of habitat suitable for this species is attributed to the same human activities that are causing habitat loss today. Ongoing and accelerated habitat destruction of the remaining Polylepis forest fragments in Peru and Bolivia continues to reduce the quantity, quality, distribution, and regeneration of remaining patches. Human activities that degrade, alter, and destroy habitat are ongoing throughout the species' range, including within protected areas (see also Factor D). Current research indicates that climate fluctuations exacerbate the risks to species that are already undergoing range reduction due to habitat alteration. Climate models predict that this species' habitat will continue to decline. Experts consider the species' population decline to be commensurate with the declining habitat (Factor E). Therefore, we find that destruction and modification of habitat are threats to the continued existence of the ash-breasted tit-tyrant throughout its range. B. Overutilization for Commercial, Recreational, Scientific, or Educational Purposes We are not aware of any information currently available that indicates that overutilization of the species for commercial, recreational, scientific, or educational purposes has occurred or is occurring at this time. As a result, we are not considering overutilization to be a threat to the continued existence of the ash- breasted tit-tyrant. C. Disease or Predation We are not aware of any scientific or commercial information that indicate disease or predation poses a threat to this species. As a result, we are not considering disease or predation to be a [[Page 613]] threat to the continued existence of the ash-breasted tit-tyrant. D. Inadequacy of Existing Regulatory Mechanisms This analysis of regulatory mechanisms is discussed on a country- by-country basis, beginning with Peru. Peru: The ash-breasted tit-tyrant is considered ``endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276855). This Decree prohibits hunting, take, transport, and trade of protected species, except as permitted by regulation. As hunting, take, transport, and trade are not currently threats to the ash-breasted tit- tyrant (Factor B), this regulation does not mitigate any current threats to this species. The Peruvian national protected area system includes several categories of habitat protection. Habitat may be designated as any of the following: (1) Parque Nacional (National Park, an area managed mainly for ecosystem conservation and recreation ); (2) Santuario (Sanctuary, for the preservation of sites of notable natural or historical importance); (3) Reserva Nacional (National Reserve, for sustainable extraction of certain biological resources); (4) Bosque de Proteccion (Protection Forest, to safeguard soils and forests, especially for watershed conservation); (5) Zona Reservada (Reserved Zone, for temporary protection while further study is under way to determine their importance); (6) Bosque Nacional (National Forest, to be managed for utilization); (7) Reserva Comunal (Communal Reserve, for local area use and management, with national oversight); and (8) Cotos de Caza (Hunting Reserve, for local use and management, with national oversight) (BLI 2008, p. 1; Rodriguez and Young 2000, p. 330). National reserves, national forests, communal reserves, and hunting reserves are managed for the sustainable use of resources (IUCN 1994, p. 2). The designations of National Parks, Sanctuaries, and Protection Forests, are established by supreme decree that supersedes all other legal claim to the land and, thus, these areas tend to provide more habitat protection. All other protected areas are established by supreme resolution, which is viewed as a less powerful form of protection (Rodriguez and Young 2000, p. 330). Protected areas have been established through regulation in at least three sites occupied by the ash-breasted tit-tyrant in Peru: Parque Nacional Huascaran (Ancash), and Santuario Historico Machu Picchu (Cusco); and Zona Reservada de la Cordillera Huayhuash (spanning Ancash, Huanuco, and Lima) (Barrio 2005, p. 563; BLI 2009i, p. 1; BLI 2009l, p. 1; BLI 2009n, p. 1). Habitat destruction and alteration, including burning, cutting, and grazing are ongoing within Parque Nacional Huascaran and Santuario Historico Machu Picchu (BLI 2009l, p. 4; BLI 2009n, p. 2; Engblom et al. 2002, p. 58), where resources are supposed to be managed for conservation (Rodriguez and Young 2000, p. 330). Reserved zones are intended to be protected pending further study (Rodriguez and Young 2000, p. 330). However, burning for habitat conversion and maintenance of pastures for grazing and increasing ecotourism are ongoing within Zona Reservada de la Cordillera Huayhuash (Barrio 2005, p. 564). Therefore, the occurrence of the ash-breasted tit-tyrant within protected areas in Peru does not protect the species, nor does it mitigate the threats to the species from ongoing habitat loss (Factor A) and concomitant population decline (Factor E). Recent studies by the Peruvian Society for Environmental Law (SPDA) have concluded that there are approximately 5,000 laws and regulations directly or indirectly related to environmental protection and natural resource conservation in Peru. However, many of these are not effective because of limited implementation and/or enforcement capability (Muller 2001, pp. 1-2). Bolivia: The 1975 Law on Wildlife, National Parks, Hunting and Fishing (Decree Law No. 12,301 1975, pp. 1-34) has the fundamental objective of protecting the country's natural resources. This law governs the protection, management, utilization, transportation, and selling of wildlife and their products; the protection of endangered species; habitat conservation of fauna and flora; and the declaration of national parks, biological reserves, refuges, and wildlife sanctuaries, tending to the preservation, promotion, and rational use of these resources (Decree Law No. 12,301 1975, pp. 1-34; eLAW 2003, p. 2). Although this law designates national protection for all wildlife, there is no information as to the actual protections this confers to ash-breasted tit-tyrants. Law No. 12,301 (1975, pp. 1-34) also placed into public trust all national parks, reserves, refuges, and wildlife sanctuaries. However, there is no specific information as to the governmental protections afforded within the protected areas to either the ash-breasted tit-tyrant or its habitat. Given the ongoing habitat destruction throughout the species' range in Bolivia, this law does not protect the species, nor does it mitigate the threats to the species from ongoing habitat loss (Factor A) and concomitant population decline (Factor E). Bolivia passed an overarching environmental law in 1992 (Law No. 1,333 1992), with the intent of protecting and conserving the environment and natural resources. However, there is no specific legislation to implement these laws (eLAW 2003, p. 1). Therefore, we cannot determine that this law protects the species or mitigates the threats to the species from ongoing habitat loss (Factor A) and concomitant population decline (Factor E). In Bolivia, habitat is protected either on the national or departmental level through the following designations: (1) Parque (Park, for strict and permanent protection of representative of ecosystems and provincial habitats, as well as plant and animal resources, along with the geographical, scenic and natural landscapes that contain them); (2) Santuario (Sanctuary, for the strict and permanent protection of sites that house endemic plants and animals that are threatened or in danger of extinction); (3) Monumento Natural (Natural Monument, to preserve areas such as those with distinctive natural landscapes or geologic formations, and to conserve the biological diversity contained therein); (4) Reserva de Vida Silvestre (Wildlife Reserve, for protection, management, sustainable use and monitoring of wildlife); (5) Area Natural de Manejo Integrado (Natural Area of Integrated Management, where conservation of biological diversity is balanced with sustainable development of the local population; and (6) Reserva Natural de Inmovilizacion (``Immobilized'' Natural Reserve, a temporary (5-year) designation for an area that requires further research before any official designations can be made and during which time no natural resource concessions can be made within the area) (Supreme Decree No. 24,781 1997, p. 3). Within parks, sanctuaries and natural monuments, extraction or consumption of all resources are prohibited, except for ``scientific research, eco- tourism, environmental education, and activities of subsistence of original towns, properly described and authorized.'' National protected areas are under the management of the national government, while departmental protected areas are managed at the department level (eLAW 2003, p. 3; Supreme Decree No. 24,781 1997, p. 3). The ash-breasted tit-tyrant occurs within several protected areas in the Department of La Paz, Bolivia: Parque Nacional y area Natural de Manejo [[Page 614]] Integrado Madidi, Parque Nacional y area Natural de Manejo Integrado Cotapata, and the co-located protected areas of Reserva Nacional de Fauna de Apolobamba, area Natural de Manejo Integrado de Apolobamba, and Reserva de la Biosfera de Apolobamba (Auza and Hennessey 2005, p. 81; BLI 2009a, p. 1; BLI 2009b, p. 1; BLI 2009c, p. 1; BLI 2009e, p. 1). Although national parks are intended to be strictly protected, the two parks in which the species occurs (Parque Nacional y area Natural de Manejo Integrado Madidi and Parque Nacional y area Natural de Manejo Integrado Cotapata) are also designated as areas of integrated management, which are managed for the biological conservation balanced with the sustainable development of the local population (Supreme Decree No. 24,781 1997, p. 3). Grazing and firewood extraction are ongoing within Parque Nacional y area Natural de Manejo Integrado Cotapata (BLI 2009b, p. 2; BLI 2009c, p. 2). Commercial logging has occurred within Parque Nacional y area Natural de Manejo Integrado Madidi (BLI 2009a, p. 2; WCMC 1998a, p. 1). Uncontrolled clearing, extensive agriculture, grazing, and ``irresponsible'' tourism are ongoing within the Apolobamba protected areas (Auza and Hennessey 2005, p. 81; BLI 2009e, p. 5). Habitat degradation and destruction from grazing, forest fires, and timber extraction are ongoing in other protected areas, such as Tunari National Park (Department of Cochabamba, Bolivia), where suitable habitat exists for this species (De la Vie 2004, p. 7). Therefore, the occurrence of the ash-breasted tit-tyrant within protected areas in Bolivia does not protect the species, nor does it mitigate the threats to the species from ongoing habitat loss (Factor A) and concomitant population decline (Factor E). Summary of Factor D Peru and Bolivia have enacted various laws and regulatory mechanisms to protect and manage wildlife and their habitats. The ash- breasted tit-tyrant is ``endangered'' under Peruvian law and occurs within several protected areas in Peru and Bolivia. As discussed under Factor A, the ash-breasted tit-tyrant prefers dense woodlands, which have been reduced by an estimated 99 percent in Peru and Bolivia, and the remaining habitat is fragmented and degraded. Habitat throughout the species' range has been and continues to be altered as a result of human activities, including clearcutting and burning for agriculture, grazing lands, and industrialization; extractive activities, including, firewood, timber, and mineral extraction; and human encroachment and concomitant increased pressure on natural resources. Despite the species' ``endangered'' status in Peru and Bolivian laws intended to protect all wildlife and natural resources, these activities are ongoing within protected areas, indicating that the laws governing wildlife and habitat protection in both countries are either inadequate or inadequately enforced to protect the species or to mitigate ongoing habitat loss (Factor A) and population declines (Factor E). Therefore, we find that the existing regulatory mechanisms are inadequate to mitigate the current threats to the continued existence of the ash- breasted tit-tyrant throughout its range. E. Other Natural or Manmade Factors Affecting the Continued Existence of the Species An additional factor that affects the continued existence of the ash-breasted tit-tyrant is the species' small population size. As discussed above (see Population Estimates), the global population estimate is not an accurate reflection of the species' effective population size because gene flow does not occur between the subspecies. At the same time, there is insufficient information on the subspecies or subpopulation level (in terms of numbers of individuals and breeding structure) to estimate the effective population size at the subspecies level. However, with an estimated global population size in the mid- to upper-hundreds (BLI 2000, p. 392; BLI 2007, p. 1; BLI 2009o, p. 1), and the most recent estimate of 641 individuals (Chutas 2007, pp. 4, 8; Gomez in litt. 2007, p. 1), the ash-breasted tit-tyrant is considered to have a ``very small population'' size (BLI 2000, p. 392; BLI 2008, p. 1; BLI 2009o, p. 1). Small population size renders a species vulnerable to any of several risks, including inbreeding depression, loss of genetic variation, and accumulation of new mutations. Inbreeding can have individual or population-level consequences either by increasing the phenotypic expression (the outward appearance or observable structure, function, or behavior of a living organism) of recessive, deleterious alleles (harmful gene sequences) or by reducing the overall fitness of individuals in the population (Charlesworth and Charlesworth 1987, p. 231; Shaffer 1981, p. 131). Small, isolated wildlife populations are also more susceptible to environmental fluctuations and demographic shifts (Pimm et al. 1988, pp. 757, 773-775; Shaffer 1981, p. 131), such as reduced reproductive success of individuals and chance disequilibrium of sex ratios. Species tend to have a higher risk of extinction if they occupy a small geographic range and occur at low density (Purvis et al. 2000, p. 1949). The ash-breasted tit-tyrant population declined at a rate between 10 and 19 percent in the past 10 years, and this decline is expected to continue in close association with continued habitat loss and degradation (see Factor A) (BLI 2009o, p. 1). Extinction risk is heightened in small, declining populations by an increased vulnerability to the loss of genetic variation due to inbreeding depression and genetic drift (changes in relative frequency of gene sequences). This, in turn, compromises a species' ability to adapt genetically to changing environments (Frankham 1996, p. 1507) and reduces fitness, thus increasing extinction risk (Reed and Frankham 2003, pp. 233-234). Once a population is reduced below a certain number of individuals, it tends to rapidly decline towards extinction (Franklin 1980, pp. 147-148; Gilpin and Soule 1986, p. 25; Holsinger 2000, pp. 64-65; Soule 1987, p. 181). Complications arising from the species' small population size are exacerbated by the species' fragmented distribution. The ash-breasted tit-tyrant is currently confined to restricted and severely fragmented forest patches in the high Andes of Peru and Bolivia (BLI 2000, p. 392; BLI 2007, pp. 1; BLI 2008, p. 1; BLI 2009o, p. 1; Collar et al. 1992, p. 753; Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113; del Hoyo et al. 2004, p. 281; InfoNatura 2007, p. 1) , where it is estimated that only 1 percent of the dense woodlands preferred by the species remain (Fjeldsa 2002a, p. 114; Smith 1971, p. 269) (see Habitat and Life History). Habitat fragmentation can cause genetic isolation and heighten the risks to the species associated with short-term genetic viability. Species with a small population size, combined with a restricted and severely fragmented range, are exposed to increased vulnerability to adverse natural events and manmade activities that destroy individuals and their habitat (Holsinger 2000, pp. 64-65; Primack 1998, pp. 279-308; Young and Clarke 2000, pp. 361-366). Summary of Factor E The ash-breasted tit-tyrant has a small population size that renders it vulnerable to genetic risks that negatively impact the species' viability. The species occurs in two disjunct [[Page 615]] populations, where habitat is highly fragmented and continues to be altered by human activities (Factor A). The restricted and fragmented range, as well as its small population size, increases the species' vulnerability to extinction, through demographic or environmental fluctuations. Based on its small population size and fragmented distribution, we have determined that the ash-breasted tit-tyrant is particularly vulnerable to the threat of adverse natural events (e.g., genetic, demographic, or environmental) and human activities (e.g., deforestation, habitat alteration, and infrastructure development) that destroy individuals and their habitat. The genetic and demographic risks associated with small population sizes are exacerbated by ongoing human activities that continue to curtail the species' habitat throughout its range. The species' population has declined and is predicted to continue declining commensurate with ongoing habitat loss (Factor A). Therefore, we find that the species' small population size, in concert with its fragmented distribution and its heightened vulnerability to adverse natural events and manmade activities, are threats to the continued existence of the ash-breasted tit-tyrant throughout its range. Status Determination for the Ash-Breasted Tit-Tyrant The ash-breasted tit-tyrant, a small New World tyrant flycatcher, exists in two disjunct areas in Peru and Bolivia. Preferring dense, semihumid Polylepis or Polylepis -mixed woodlands, the ash-breasted tit-tyrant occupies a narrow range of distribution, at elevations between 12,139 and 15,092 ft (3,700 and 4,600 m). The species has a highly restricted and severely fragmented range (approximately 4,595 mi\2\ (11,900 km\2\)), and is known only in two disjunct areas: one location in west-central Peru (in the Peruvian Administrative Regions of Ancash, Huanuco, La Libertad, and Lima) and another location ranging from southern Peru (Apurimac, Cusco, Puno, and Arequipa Regions) into northern Bolivia (in the Department of La Paz). The known population of the ash-breasted tit-tyrant is estimated to be 641 individuals. We have carefully assessed the best available scientific and commercial information regarding the past, present, and potential future threats faced by the ash-breasted tit-tyrant and have concluded that there are three primary factors that threaten the continued existence of the ash-breasted tit-tyrant: (1) Habitat destruction, fragmentation, and degradation; (2) limited size and isolation of remaining populations; and (3) inadequate regulatory mechanisms. Human activities that degrade, alter, and destroy habitat are ongoing throughout the ash-breasted tit-tyrant's range. Widespread deforestation and the conversion of forests for grazing, agriculture, and human settlement have led to the fragmentation and degradation of habitat throughout the range of the ash-breasted tit-tyrant (Factor A). Researchers estimate that only 1 percent of the dense Polylepis woodlands preferred by the species remain extant. Limited by the availability of suitable habitat, the species occurs today only in some of these fragmented and disjunct locations. Ash-breasted tit-tyrant habitat continues to be altered by human activities, such as burning, grazing, extractive activities, and human encroachment, which result in the continued degradation, conversion, and destruction of habitat and reduce the quantity, quality, distribution, and regeneration of remaining forest patches. The ash-breasted tit-tyrant population is small, rendering the species particularly vulnerable to the threat of adverse natural events (e.g., genetic, demographic, or environmental) and human activities (e.g., deforestation and habitat alteration) that destroy individuals and their habitat. Ongoing human activities that curtail the species' habitat throughout its range exacerbate the genetic and demographic risks associated with small population sizes (Factor E). The population has declined 10-19 percent in the past 10 years (see Population Estimates), and is predicted to continue declining commensurate with ongoing habitat loss (Factor A). Habitat loss was a factor in the ash- breasted tit-tyrant's historical population decline (see Historical Range and Distribution), and the species is considered to be declining today in association with the continued reduction in habitat (Factors A and E). Moreover, current research indicates that narrow endemics, such as the ash-breasted tit-tyrant, are especially susceptible to climate fluctuations, because of the synergistic effect these fluctuations have on declining populations that are also experiencing range reductions due to human activities (Factor A). Despite the species' ``endangered'' status in Peru and its occurrence within several protected areas in Peru and Bolivia (Factor D), human activities that degrade, alter, and destroy habitat are ongoing throughout the species' range, including within protected areas. Therefore, regulatory mechanisms are either inadequate or ineffective at curbing the threats to the ash-breasted tit-tyrant of habitat loss (Factor A) and corresponding population decline (Factor E). Section 3 of the Act defines an ``endangered species'' as ``any species which is in danger of extinction throughout all or a significant portion of its range'' and a ``threatened species'' as ``any species which is likely to become an endangered species within the foreseeable future throughout all or a significant portion of its range.'' Based on the immediate and ongoing threats to the ash-breasted tit-tyrant throughout its entire range, as described above, we determine that the ash-breasted tit-tyrant is in danger of extinction throughout all of its range. Therefore, on the basis of the best available scientific and commercial information, we are proposing to list the ash-breasted tit-tyrant as an endangered species throughout all of its range. II. Junin grebe (Podiceps taczanowskii) Species Description The Junin grebe is a highly social, flightless water bird in the Podicipedidae family that is endemic to a single location (Lake Junin) in Peru. Other common names for the species (in English) are: Junin flightless grebe, puna grebe, and Taczanowski's grebe. This species is also known by two Spanish names: ``zampullin del Junin'' or ``zambullidor de Junin'' (del Hoyo et al. 1992, p. 195; Fjeldsa 2004, p. 199; Instituto Nacional de Recursos Naturales (INRENA) 1996, p. 3; Ramsen et al. 2007, p. 18; Supreme Decree 034-2004-AG 2004, p. 276854). A slim, long-necked bird, the Junin grebe is about 13.78 in (35 cm) in length, and its weight ranges from 0.66 to 1.04 pounds (0.30 to 0.47 kilograms) (BLI 2009b, p. 1; UNEP-WCMC 2009, p. 1). The Junin grebe has a pointed head, with dark feathers on its back, a white throat, and mottled, dusky-colored underparts. This grebe is distinguished by its slender gray bill, red iris, and dull yellow-orange colored feet. Immature birds are darker gray on the flanks than mature birds (BLI 2009b, p. 1). Taxonomy The Junin grebe was taxonomically described by Berlepsch and Stolzmann in 1894 (ITIS 2009, p. 1). It is one of nine species of grebes in the genus Podiceps worldwide (Dickinson 2003, p.80). The species' taxonomic status as Podiceps taczanowskii is valid (ITIS 2009, p. 1). [[Page 616]] Habitat and Life History The Junin grebe is endemic to the open waters and marshlands of Lake Junin, located at 13,390 ft (4,080 m) above sea level in the Peruvian Administrative Region of Junin (BLI 2003, p. 1; BLI 2009b, p. 1). The 57-mi\2\ (147-km\2\) lake, also known as ``Chinchaycocha'' or ``Lago de Junin,'' is large but fairly shallow (BLI 2003, p. 1; BLI 2009a, p. 1; BLI 2009b, p. 1; ParksWatch 2009, p. 1; Tello 2007, p. 1). Situated within ``puna'' habitat, the climate is seasonal and can be ``bitterly cold'' in the dry season (Fjeldsa 1981, p. 240). Local vegetation is characterized by tall dense grasslands and scrubland with open, rocky areas, all interspersed with wetlands and woodlands (BLI 2003, p. 1; ParksWatch 2009, pp. 1, 4). The dominant terrestrial plant species surrounding the lake includes 43 species of grass (Poaceae family), 15 species of asters (Asteraceae family), and 10 species of legumes (Fabaceae family) (ParksWatch 2009, p. 1). Aquatic vegetation includes Andean watermilfoil (Myriophyllum quitense), several species of pondweed (including Elodea potamogeton, Potamogeton ferrugineus, and P. filliformis), and bladderwort (Utricularia spp.). Floating plants, such as duckweed (Lemna species (spp.)), large duckweed (Spiodela spp.), and water fern (Azolla filiculoides), also occur on the lake (ParksWatch 2009, p. 2). The Lake is surrounded by extensive marshland along the lake shore (BLI 2009a, p. 1; BLI 2009b, p. 1) that extends into the lake up to 1-3 mi (2-5 km) from shore (O'Donnel and Fjeldsa 1997, p. 29). The marshes are dominated by two robust species of cattails, giant bulrush (Schoenoplectus californicus var. totara) and totorilla (Juncus articus var. andicola) (Fjeldsa 1981, pp. 244, 246). Both cattail species can reach nearly 6.6 ft (2 m) in height. These plant communities, or ``tortoras,'' grow so densely that stands are often impenetrable (ParksWatch 2009, p. 1). In shallow water, during low lake levels, ``tortora'' communities can become partially or completely dry (BLI 2009b, p. 1; ParksWatch 2009, p. 2). Lake Junin supports one of the richest and most diverse arrays of bird species of all Peruvian high Andean wetlands (ParksWatch 2009, p. 3). These bird species include migratory birds, birds that nest at high altitude, aquatic birds, and local endemic species, such as the Junin grebe and the Junin rail (Laterallus tuerosi; also the subject of this proposed rule), the giant coot (Fulica ardesiaca), and the Chilean flamingo (Phoenicopterus chilensis) (BLI 2009a, pp. 2-3; ParksWatch 2009, p. 3; Tello 2007, p. 2). Mammals are relatively scarce in the area, although there are some predators (ParksWatch 2009, p. 4) (see Factor C). Breeding season for this species occurs annually from November to March (Fjeldsa 1981, pp. 44, 246; O'Donnel and Fjeldsa 1997, p. 29). The Junin grebe nests in the protective cover of the marshlands during the breeding season (Fjeldsa 1981, p. 247; Tello 2007, p. 3), particularly in stands of giant bulrush (ParksWatch 2009, p. 4). Under natural conditions, winter rains increase the lake water level during the breeding season, allowing the grebes to venture into local bays and canals, although they are never found nesting on the lake's shore (Tello 2007, p. 3). The species nests in the giant bulrush marshlands (ParksWatch 2009, p. 4). Well-hidden floating nests can contain up to three eggs, with an average of two eggs, laid during November and December (Fjeldsa 1981, p. 245). The species is believed to have a deferred sexual maturation (Fjeldsa 2004, p. 201) and exhibits low breeding potential, perhaps as a reflection to adaptation to a ``highly predictable, stable environment'' (del Hoyo et al. 1992, p. 195), laying one clutch during the breeding season (ParksWatch 2009, p. 4). Junin grebes occasionally produce a replacement clutch if their original nest is disturbed (Fjeldsa 2004, pp. 199, 201). After the eggs hatch, the male grebe cares for the chicks, and does not leave the nest to feed. The female grebe is responsible for feeding the male and chicks until the chicks can leave the nest (Tello 2007, p. 3). The Junin grebe is likely a long-lived species (Fjeldsa 2004, p. 201), and its breeding success and population size are highly influenced by the climate (BLI 2008, pp. 1, 3-4; BLI 2009b, p. 2; Elton 2000, p. 3; Fjeldsa 2004, p. 200; Hirshfeld 2007, p. 107) (see Factor A). The Junin grebe feeds in the open waters of the lake and around the marsh edges, moving into the open waters of the lake to feed where it is easier to dive for food during the winter (Fjeldsa 1981, pp. 247- 248; Tello 2007, p. 3). Fish (primarily pupfish (Orestias spp.)) account for over 90 percent of the grebe's diet (Fjeldsa 1981, pp. 251- 252). Pupfish become scarce when the marshlands dry during periods of reduced water levels, and the Junin grebe is then known to vary its diet with midges (Order Diptera), corixid bugs (Trichocorixa reticulata), amphipods (Hyalella simplex), and shore fly maggots and pupa (Ephydriid spp.). Historical Range and Distribution The Junin grebe was historically known to be endemic to Lake Junin, in the Peruvian Administrative Region of Junin (Fjeldsa 1981, p. 238; Fjeldsa 2004, p. 200; Fjeldsa and Krabbe 1990, p. 70; INRENA 1996, p. 1). Experts believe that the species was previously distributed throughout the entire 57-mi\2\ (147-km\2\) lake (BLI 2003, p. 1; BLI 2009a, p. 1; Fjeldsa 1981, p. 254; F. Gill and R.W. Storer, pers. comm. as cited in Fjeldsa 2004, p. 200). In 1938, the Junin grebe was encountered throughout the entire lake (Morrison 1939, p. 645). The Junin grebe is now absent from the northwest portion of Lake Junin due to mine waste contamination and a severe decline in population (Fjeldsa 1981, p. 254; F. Gill and R.W. Storer, pers. comm. as cited in Fjeldsa 2004, p. 200). Current Range and Distribution The Junin grebe continues to be endemic to the 57-mi\2\ (147-km\2\) Lake Junin, located at 13,390 ft (4,080 m) above sea level in the Peruvian High Andes (BLI 2003, p. 1; BLI 2009a, p. 1; BLI 2009b, p. 1). Although BirdLife International (2009b, p. 1) reports the current estimated range of the species as 55 mi\2\ (143 km\2\), their definition of a species' range is the total area within its extent of occurrence (see Current Range and Distribution of the ash-breasted tit- tyrant) (BLI 2000, pp. 22, 27). Noting that Lake Junin is only a 57- mi\2\ (147-km\2\) lake (BLI 2003, p. 1; BLI 2009a, p. 1) and that the Junin grebe is restricted to the southern portion of the lake (Fjeldsa 1981, p. 254; F. Gill and R.W. Storer, pers. comm. as cited in Fjeldsa 2004, p. 200), its current range is actually smaller than the figure reported by BirdLife International. The entire population of this species is located within a protected area, the Junin National Reserve (BLI 2008, p. 2; BLI 2009a, p. 1; BLI 2009b, p. 1; ParksWatch 2009, p. 4). Population Estimates The current population of the Junin grebe is estimated to be 100- 300 individuals (BLI 2009b, p. 3), having undergone a severe population decline in the latter half of the 20th century, with extreme population fluctuations during this time (Fjeldsa 1981, p. 254). Field studies in 1938 indicated that the Junin grebe was ``extremely abundant'' throughout Lake Junin (Morrison 1939, p. 645). Between 1961 and 1979 the population fell from greater than 1,000 individuals to an estimated 250-300 birds (BLI 2009b, p. 2; Collar et al. 1992, p. 43; Harris 1981, as cited in O'Donnell and Fjeldsa 1997, p. 30; Fjeldsa 1981, p. [[Page 617]] 254). Surveys during the mid-1980s estimated a total of 250 individuals inhabiting the southern portion of Lake Junin (BLI 2009b, p. 2; Collar et al. 1992, p. 43). In 1992, only 100 birds were observed and, by 1993, the population had declined to 50 birds, of which fewer than half were breeding adults (BLI 2008, p. 3; BLI 2009b, p. 2). In 1995, an estimated 205 Junin grebes were present on Lake Junin (O'Donnell and Fjeldsa 1997, p. 30). Breeding and fledging were apparently unsuccessful from 1995 to 1997. However, there were two successful broods fledged during the 1997 and 1998 breeding seasons (BLI 2008, p. 3; T. Valqui in litt., as cited in BLI 2009b, p. 2). In 1998, more than 250 Junin grebes were counted in a 1.5-mi\2\ (4-km\2\) area in the southern portion of Lake Junin, suggesting a total population of 350 to 400 birds (T. Valqui in litt., as cited in BLI 2009b, p. 2). In 2001, field surveys indicated that there may have been a total population of 300 birds, but that estimate has been considered overly optimistic (Fjeldsa in litt. 2003, as cited in BLI 2009b, p. 2). Fjeldsa (in litt. 2003, as cited in BLI 2009b, p. 2) postulated that perhaps only half that number would have been mature individuals. These population fluctuations are strongly linked to precipitation (see Factor A). The species has experienced a population decline of 14 percent in the past 10 years, and is expected to continue to decline (BLI 2009b, pp. 1, 6-7). The species' decline is associated with continued habitat loss and degradation (Fjeldsa 1981, p. 254; F. Gill and R.W. Storer, pers. comm. as cited in Fjeldsa 2004, p. 200) (See Factors, A, C, and E). Conservation Status The Junin grebe is considered ``critically endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276853). The IUCN categorizes the Junin grebe as ``Critically Endangered'' because it is endemic to one location and has undergone ``significant population declines, such that an extremely small number of adults remain'' (BLI 2008, p. 1; BLI 2009b, pp. 1, 3). The single known population of the Junin grebe occurs wholly within one protected area in Peru, the Junin National Reserve (BLI 2009b, pp. 1-2). Summary of Factors Affecting the Junin Grebe A. The Present or Threatened Destruction, Modification, or Curtailment of the Species' Habitat or Range The Junin grebe is endemic to Lake Junin, where it resides year- round. The species is completely dependent on the open waters and marshland margins of the lake for feeding and on the protective cover of the marshlands during the breeding season (BLI 2008, p. 1; BLI 2009a, p. 1; Fjeldsa 1981, p. 247; Tello 2007, p. 3). The current estimated range of the species is 55 mi\2\ (143 km\2\) (BLI 2009b, p. 1). However, as described under Current Range and Distribution, its actual range is smaller, because the species is restricted to the southern portion of the lake (BLI 2009b, p. 1; Fjeldsa 1981, p. 254; F. Gill and R.W. Storer, pers. comm. as cited in Fjeldsa 2004, p. 200). Breeding season begins in November (Fjeldsa 1981, pp. 44, 246; O'Donnel and Fjeldsa 1997, p. 29). Junin grebes build their nests (BLI 2008, p. 1; Fjeldsa 1981, p. 247; Tello 2007, p. 3) and obtain their primary prey, pupfish, in the expansive offshore flooded marshlands that may extend into the lake up to 1-3 mi (2-5 km) from shore (Fjeldsa 2004, p. 200; O'Donnel and Fjeldsa 1997, pp. 29-30). The quality of Junin grebe habitat and the species' reproductive success is highly influenced by water levels and the water quality of the lake. Water levels in the lake are affected by manmade activities (such as hydropower generation) that are exacerbated by unpredictable climate fluctuations (such as drought or excessive rain). Water quality in Lake Junin has been compromised by contamination. The Upamayo Dam, located at the northwest end of the lake, has been in operation since 1936, and lake water is used to power the 54- megawatt Malpaso hydroelectric plant (Martin et al. 2001, p. 178; ParksWatch 2006, p. 5). Dam operations have caused seasonal water level fluctuations of as much as 6 ft (2 m) in Lake Junin (Martin and McNee 1999, p. 659). Under normal conditions, water levels are lower in the dry season and the marshlands can become partially or completely dry (BLI 2009b, p. 1; ParksWatch 2009, p. 2). The floodgates of the dam are often opened during the dry season (June to November) (BLI 2009b, p. 1; ParksWatch 2009, p. 2), and water offtake for hydropower generation further drains the lake, such that by the end of the dry season, in November, the marshlands encircling the lake are more apt to become completely desiccated (Fjeldsa 2004, p. 123). Reduced water levels directly impact the Junin grebe's breeding success, by reducing the amount of available nesting habitat (BLI 2008, p. 1; Fjeldsa 2004, p. 200). The giant bulrush marshlands, upon which the Junin grebe relies for nesting and foraging habitat, have virtually disappeared from some sections of the lake (O'Donnel and Fjeldsa 1997, p. 29). When the marshlands are completely desiccated, the Junin grebe does not breed at all (Fjeldsa 2004, p. 123). Reduced water levels also impact the species by reducing the Junin grebe's primary prey, pupfish (Fjeldsa 2004, p. 200) (see Habitat and Life History). The perimeter of the flooded marshlands provides the primary recruitment habitat for fish in the lake during extremely dry years, including 1983-1987, 1991, and 1994-1997 (Fjeldsa 2004, p. 200; O'Donnel and Fjeldsa 1997, p. 29). Submerged aquatic vegetation, habitat for pupfish, has become very patchy, further triggering declines in the prey population. Few marshlands are permanently inundated now, due to the power generation requirements of the Upamayo Dam, and the giant bulrushes that previously grew tall and provided extensive cover for this species for breeding and feeding have virtually disappeared, reducing both nesting and foraging habitat for the Junin grebe. The reduction in nesting and foraging habitat are believed to contribute to mass mortality of Junin grebes during extreme drought years (O'Donnel and Fjeldsa 1997, p. 30). Manipulation of the Lake Junin's water levels also results in competition between the white-tufted grebe (Rollandia rolland) and the Junin grebe for food resources during the Junin grebe's breeding season (Fjeldsa 2004, p. 200). Under normal conditions, the expansive offshore marshlands may extend into the lake up to 1-3 mi (2-5 km) from shore (O'Donnel and Fjeldsa 1997, p. 29). In years when water levels remain high, the Junin grebe and white-tufted grebe are spatially segregated during the breeding season, with white-tufted grebes utilizing the interior of the reed marsh and Junin grebes remaining at the edges of the marshlands, closer to the center of the lake (Fjeldsa 1981, pp. 245, 255). Near the end of the dry season, as early as October, when water levels are lower in the lake and the marshlands can partially or completely dry out (BLI 2009b, p. 1; ParksWatch 2009, p. 2), thousands of white-tufted grebes move from the interior of the marshlands to the edges, where they compete with the Junin grebe for food [[Page 618]] (Fjeldsa 1984, pp. 413-414). As the breeding season for the Junin grebe begins in November (Fjeldsa 1981, pp. 44, 246; O'Donnel and Fjeldsa 1997, p. 29), Junin grebes build floating nests and breed on the margins of marshlands (BLI 2008, p. 1; Fjeldsa 1981, p. 247; Tello 2007, p. 3), and a plentiful supply of fish becomes more important (O'Donnel and Fjeldsa 1997, p. 29). Competition becomes more critical the longer the water level remains low at the end of the dry season, and activities that further reduce low water levels only accentuate this competition (Fjeldsa 1981, pp. 252-253). Water quality affects the availability of habitat for the endemic Junin grebe. The water in Lake Junin has been contaminated from mining waste, agricultural runoff, and organic matter from the land surrounding the lake. There are several mining operations (lead, copper, and zinc) north of Lake Junin, and wastewater from the mines flows untreated into the lake via the Rio San Juan (Fjeldsa 1981, p. 255; Martin and McNee 1999, pp. 660-661; ParksWatch 2006, p. 2; Shoobridge 2006, p. 3). Agricultural insecticides wash into Lake Junin from surrounding fields and through drainage systems from villages around the lake (ParksWatch 2006, pp. 5, 19). Organic matter originating from local communities is piped untreated into the lake, resulting in eutrophication (a process whereby excess nutrients facilitate excessive plant growth, which ultimately reduces the amount of dissolved oxygen in the water, harming oxygen-dependent organisms) (ParksWatch 2006, p. 5; Shoobridge 2006, p. 3). Lake Junin is a sink for several streams that transport mining wastes and other pollution downstream and into the lake (ParksWatch 2006, p. 19). The Rio San Juan is the primary source of water for Lake Junin and feeds into the lake from the northern end (Fjeldsa 1981, p. 255; Martin and McNee 1999, pp. 660-661; Shoobridge 2006, p. 3). Tests indicate that the Rio San Juan contains trace metals, including copper, lead, mercury, and zinc, in excess of currently accepted aquatic life thresholds (Martin and McNee 1999, pp. 660-661). Non-point source pollutants from agricultural fertilizers, such as ammonium and nitrate concentrations, are also suspended in the water column (Martin and McNee 1999, pp. 660-661). Iron oxide contamination is visible near the outflow of the Rio San Juan because iron oxide produces a reddish tinge to the water and reed borders. Vegetation near the river's outflow is completely absent (Fjeldsa 2004, p. 124; ParksWatch 2006, pp. 20-21), and this portion of the lake has been rendered lifeless by the precipitation of iron oxide from mining wastewaters (BLI 2008, p. 4). The giant bulrush marshlands, which once existed in great expanses around the entire perimeter of the lake and upon which the Junin grebe relies for nesting and foraging habitat, have virtually disappeared and at least one species of catfish (Pygidium oroyae) may have been extirpated from the lake (O'Donnel and Fjeldsa 1997, p. 29). Heavy metal contamination is not limited to the northern end of the lake (ParksWatch 2006, p. 20), but extends throughout the southern end (Martin and McNee 1999, p. 662), where the Junin grebe is now restricted (BLI 2003, p. 1; BLI 2009b, p. 1; Fjeldsa 1981, p. 254; F. Gill and R.W. Storer, pers. comm. as cited in Fjeldsa 2004, p. 200). Near the center of the lake, the bottom has been described as ``lifeless,'' due to sedimentation of iron oxides (Fjeldsa 1981, pp. 255-256; Fjeldsa 2004, p. 124). Martin et al. (2001, p. 180) determined that sediments at the lake's center are enriched with copper, zinc, and lead and are anoxic (having low levels of dissolved oxygen). High concentrations of dissolved copper, lead, and zinc have damaged an estimated one-third of the lake, increasing turbidity of the lake, and exceeding established aquatic life thresholds (Martin and McNee 1999, pp. 660-661; ParksWatch 2006, pp. 2, 20; Shoobridge 2006, p. 3). This has severely affected animal and plant populations in the area, contributing to mortality of species, including the Junin grebe, around the lake (ParksWatch 2006, pp. 3, 20) (see Factor C). In 2009, conservation organizations and civil society groups demanded action to reverse the deterioration of Lake Junin and requested an independent environmental audit and continuous monitoring of the lake (BLI 2009b p. 4; BLI 2009c, p. 1). The conservation groups BLI, American Bird Conservancy (ABC), Asociacion Ecosistemas Andinos (ECOAN), and INRENA adopted the Junin grebe as the symbol of wetland conservation for the high Andes (BLI 2009c, p. 1). Although translocation has been a consideration for the conservation of the Junin grebe since the mid-1990s, no suitable habitat for the species has been located (O'Donnel and Fjeldsa 1997, pp. 30, 35; BLI 2008, p. 5; BLI 2009b, p. 2). None of these conservation organization's activities have been effective at curbing the ongoing habitat degradation (see also Factor D). The effects of habitat alteration and destruction (such as those caused by artificially reduced water levels and water contamination) are accentuated by unpredictable climate fluctuations (such as droughts or excessive rains) (Jetz et al. 2007, pp. 1211, 1213; Mora et al. 2007, p. 1027). Peru is subject to unpredictable climate fluctuations, such as those that are related to the El Ni[ntilde]o Southern Oscillation (ENSO). Changes in weather patterns, such as ENSO cycles (El Ni[ntilde]o and La Ni[ntilde]a events), tend to increase precipitation in normally dry areas, and decrease precipitation in normally wet areas (Holmgren et al. 2001, p. 89; TAO Project n.d., p. 1); thereby exacerbating the effects of habitat reduction and alteration on the decline of a species (England 2000, p. 86; Holmgren et al. 2001, p. 89; Jetz et al. 2007, pp. 1211, 1213; Mora et al. 2007, p. 1027; Parmesan and Mathews 2005, p. 334; Plumart 2007, pp. 1-2; Timmermann 1999, p. 694), especially for narrow endemics (Jetz et al. 2007, p. 1213) such as the Junin grebe (see also Factor E). Moreover, the Junin grebe's low breeding potential is considered to be a reflection of its adaptation to being in a ``highly predictable, stable environment'' (del Hoyo et al. 1992, p. 195). The Junin grebe's breeding success and population size are highly influenced by the climate, with population declines occurring during dry years, population increases during rainy years, and mortality during extreme cold weather events. Several times during the last two decades (e.g., 1983-1987, 1991-1992, 1994-1997), the population has declined to 100 birds or less following particularly dry years (BLI 2008, pp. 1, 3-4; BLI 2009b, p. 2; Elton 2000, p. 3; Fjeldsa 2004, p. 200). There have been short-term population increases of 200 to 300 birds in years with higher rainfall amounts following El Ni[ntilde]o events (such as the 1997-1998 and 2001-2002 breeding seasons) (PROFONANPE 2002, as cited in Fjeldsa 2004, p. 133; T. Valqui pers. comm., as cited in BLI 2009b, p. 2). However, excessive rains also increase contamination in Lake Junin, which decreases the amount of suitable habitat for the species (as described above) and has adverse effects on the species' health (see Factor C). Many Junin grebes died during extremely cold conditions in 1982 (BLI 2008, p. 4). In 2007, the population declined again following another cold weather event (Hirshfeld 2007, p. 107). ENSO cycles are ongoing, having occurred several times within the last decade (NWS 2009, p. 2), and evidence suggests that ENSO cycles have already increased in periodicity and severity (Richter 2005, pp. 24-25; [[Page 619]] Timmermann 1999, p. 694), which will exacerbate the negative impacts of habitat destruction on a species. Habitat degradation and alteration caused by fluctuating water levels and environmental contamination are considered key factors in the species' historical decline (Fjeldsa 1981, p. 254; F. Gill and R.W. Storer, pers. comm. as cited in Fjeldsa 2004, p. 200). The species has experienced a population decline of 14 percent in the past 10 years, and is expected to continue to decline as a result of deteriorating habitat and water quality (BLI 2009b, pp. 1, 6-7). Therefore, further habitat degradation is expected to continue impacting this species' already small population size (see Factor E). Summary of Factor A The habitat in and around Lake Junin, where the Junin grebe is endemic, has been and continues to be altered and degraded as a result of human activities, including artificial water level fluctuations to generate hydropower and water contamination caused by mining waste, agricultural and organic runoff from surrounding lands, and wastewater from local communities. The Junin grebe is dependent on the quantity and quality of lake water for breeding and feeding. Water levels in Lake Junin are manipulated to generate electricity, which leads to dramatic fluctuations in water levels of up to 6 ft (1.8 m). The Junin grebe relies on the protective cover of flooded marshlands for nesting. As water drawdown occurs near the end of the dry season and the inception of the Junin grebe's mating season, portions of the marshlands may dry out completely. Reductions in water levels decrease the availability of suitable breeding and foraging habitat, and decrease the availability of the Junin grebe's primary prey, forcing competition with the white-tufted grebe for food. Drought years have a negative impact on the Junin grebe, resulting in severe population fluctuations due to poor breeding success and limited recruitment of juveniles into the adult population. Severed dry conditions can cause total breeding failure (see also Factor E). Although the population appears to rebound during wetter years (i.e., following El Ni[ntilde]o events) (see Habitat and Life History and Population Estimate), excessive rain decreases the suitable habitat for the species, as pollution washes into the water from around the lake and the upstream rivers that feed the lake, increasing contamination levels in Lake Junin. This increased contamination also affects the Junin grebe's health and has resulted in mortality of the species (see Factor C). Severe water contamination has rendered the northwest portion of the lake lifeless, devoid of aquatic and terrestrial species. Experts believe that the Junin grebe once inhabited the entire Lake, but the species is now confined to the southern portion of the lake due to water contamination (Historical Range and Distribution). Elevated levels of heavy metals may reduce the fitness and overall viability of the Junin grebe (Factor C), which would heighten risks associated with short- and long-term genetic viability (Factor E). Therefore, we find that destruction and modification of habitat are threats to the continued existence of the Junin grebe throughout its range. B. Overutilization for Commercial, Recreational, Scientific, or Educational Purposes We are not aware of any information currently available that indicates that overutilization of Junin grebe for commercial, recreation, scientific, or education purposes has occurred or is occurring at this time. Fjeldsa (1981, pp. 254-255) notes that local hunters are not interested in grebes as food because they have ``too little meat.'' As a result, we are not considering overutilization to be a threat to the continued existence of the Junin grebe. C. Disease or Predation Disease: Although no specific disease threat has been identified for the Junin grebe, contamination of Lake Junin has contributed directly and indirectly to Junin grebe mortality and has potentially reduced the overall fitness and health of the species. As discussed under Factor A, lead, copper, and zinc mining residues (Fjeldsa 1981, p. 255; Martin and McNee 1999, pp. 660-661; Shoobridge 2006, p. 3), agricultural runoff, organic matter, and wastewater are discharged directly into Lake Junin (ParksWatch 2006, pp. 5, 19; Shoobridge 2006, p. 3). High concentrations of environmental contaminants (including ammonium, copper, iron oxide, lead, mercury, nitrate, and zinc) have been detected throughout the lake (Fjeldsa 1981, pp. 255-256; Fjeldsa 2004, p. 124; Martin and McNee 1999, pp. 660-662; ParksWatch 2006, pp. 20-21) and exceed established thresholds for aquatic life (Martin and McNee 1999, pp. 660-661; ParksWatch 2006, p. 20). Chemical waste has rendered the northern portion of the lake lifeless due to eutrophication (BLI 2008, p. 4; Shoobridge 2006, p. 3) and the sediments in the center of the lake anoxic (containing no dissolved oxygen) (Martin et al. 2001, p. 180). High concentrations of suspended particulate matter increases the turbidity of the water, making it less penetrable to sunlight and resulting in die-off of aquatic plants and algae (ParksWatch 2006, p. 20). Chemical waste has damaged at least one third of the lake and has severely affected animal and plant populations in the area (O'Donnel and Fjeldsa 1997, p. 29; ParksWatch 2006, pp. 3, 20; Shoobridge 2006, p. 3). The northern portion of the lake is completely devoid of vegetation (Fjeldsa 2004, p. 124; ParksWatch 2006, pp. 20-21), and the giant bulrush marshlands, which once existed in great expanses around the entire perimeter of the lake and upon which the Junin grebe relies for nesting and foraging habitat, have virtually disappeared. At least one species of catfish (Pygidium oroyae) may have been extirpated from the lake (O'Donnel and Fjeldsa 1997, p. 29). During years of heavy rainfall, the lake is filled, and the lakeshore becomes polluted with ``toxic acid gray sediment'' that has caused large-scale mortality of cattle (approximately 2,000 in 1994) and birds, apparently due to lead poisoning (O'Donnel and Fjeldsa 1997, p. 30). Lead poisoning from the presence of mine wastes is a common cause of mortality in waterbirds, and is medically described as an intoxication resulting from absorption of hazardous levels of lead into body tissues (Friend and Franson 1999, p. 317). Water contamination has directly affected the health of the Junin grebe population. As predators of aquatic organisms, the Junin grebe occupies a mid-tertiary level position in the food chain and is prone to bioaccumulation of pesticides, heavy metals, and other contaminants that are absorbed or ingested by its prey (Fjeldsa 1981, pp. 255-256; Fjeldsa 2004, p. 123). Green plants form the first trophic, or feeding, level; they are the primary producers. Herbivores form the second trophic level, while carnivores form the third and even fourth trophic levels (The University of the Western Cape 2009, p. 1). Moreover, species such as the Junin grebe, which inhabit high trophic levels, are strictly dependent upon the functioning of a multitude of ecosystem processes. The loss or absence of species at lower trophic levels can result in cascading ecosystem effects, causing imbalances in the food web at all higher trophic levels (The University of the Western Cape 2009, p. 1). In parts of the lake, increased turbidity has caused die- off of aquatic plants and algae, disrupting the food chain (ParksWatch 2006, p. 20). Studies indicate that lead mining effluents severely reduce or eliminate primary prey populations of [[Page 620]] fish and aquatic invertebrates, either directly through lethal toxicity, or indirectly through toxicity to their prey species (Demayo et al. 1982, as cited in Eisler 1988, p. 5). Analysis of feathers and bone tissue of Junin grebes and of pupfish, the species' primary prey, indicate that both the grebe and its prey contain elevated lead levels (Fjeldsa 1981, pp. 255-256). Drought conditions exacerbate the effects of water contamination and bioaccumulation of contaminants in aquatic species. From 1989 to 1992, an extensive drought occurred in the Lake Junin area. During that time, many dead Junin grebes and other waterbirds were found along the edges of the lakeshore (T. Valqui and J. Barrio in litt. 1992, as cited in Collar et al. 1992, p. 45, 190). In 1992, one of the driest years in decades, up to 10 dead grebes per month were reported around the lake. Three Junin grebe carcasses were found along 1.2 mi (2 km) of shoreline in one month alone (T. Valqui and J. Barrio in litt. 1992, as cited in Collar et al. 1992, p. 45). Experts consider the cause of death to have been either heavy metal contamination, which increased in concentration as water levels decreased (T. Valqui and J. Barrio in litt. 1992, as cited in Collar et al. 1992, p. 45), or reduced prey availability (Fjeldsa 2004, p. 124). Reduced prey availability is exacerbated by manmade activities that are reducing the water levels of the lake, increasing competition among sympatric grebe species (different species that occupy the same range) and decreasing the marshlands that provide primary spawning habitat for the pupfish, the grebe's primary prey species (Factor A). Persistent exposure to contaminants can contribute to a decline in fitness for long-lived, mid-trophic level species, which is inherited by offspring and can impact embryonic development, juvenile health, or viability (Rose 2008, p. 624). The excessive contaminant load in Lake Junin could also allow opportunistic bacterial and viral infections to overcome individuals. According to Fjeldsa (1981, p. 254), the Junin grebe bears a heavy infestation of stomach nematodes (parasitic roundworms), especially as compared to other grebe species. Stomach contents of Junin grebes that have been examined had an average of 16.7 nematodes, compared with no nematodes in silver grebes and 1.6 nematodes in white-tufted grebes. Fjeldsa (1981, p. 254) postulates that the higher nematode infestation in Junin grebes may be an indicator of poor health. Predation: Predators around Lake Junin include the Andean fox (Pseudalopex culpaues), the long-tailed weasel (Mustela frenata), Pampas cat (Onicifelis colocolo), and hog-nosed skunk (Conepatus chinga) (ParksWatch 2009, p. 4). However, nest sites of the Junin grebe are generally inaccessible to mammalian predators (Fjeldsa 1981, p. 254). The only raptor likely to take a grebe on Lake Junin is the Cinereus harrier (Circus cinereus), which primarily feeds in white- tufted grebe habitats. Moorhens (Gallinula chloropus), which also inhabit the lake (ParksWatch 2009, p. 3; Tello 2007, p. 2), are egg stealers and may steal Junin grebe eggs for food (Fjeldsa 1981, p. 254). However, there is no direct evidence of predation upon the Junin grebe. Summary of Factor C Environmental contamination poses direct and indirect threats to the Junin grebe's overall health and survival. The species' trophic level also exposes it to bioaccumulation of toxins accumulated in the tissue of prey species. Research indicates that the species has elevated lead levels and carries a high load of nematodes, a possible indicator of overall poor health. Junin grebes have died as a direct result of contaminant poisoning or reduction in the pupfish, which has also been found to carry elevated lead levels. Therefore, we find that disease is a threat to the continued existence of the Junin grebe. However, there is no available evidence to indicate that predation is causing declines in Junin grebe populations or otherwise contributing to the species' risk of extinction. Therefore, we find that predation is not a threat to the Junin grebe. D. Inadequacy of Existing Regulatory Mechanisms The Junin grebe is listed as ``critically endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276853). This Decree prohibits hunting, take, transport, and trade of protected species, except as permitted by regulation. As hunting, take, transport, and trade do not currently threaten the Junin grebe, this regulation does not mitigate any current threats to this species. Peru has several categories of national habitat protection, which were described above as part of the Factor D analysis for the ash- breasted tit-tyrant (BLI 2008, p. 1; IUCN 1994, p. 2; Rodriguez and Young 2000, p. 330). The Junin grebe population occurs wholly within one protected area: the Junin National Reserve (Junin, Peru) (BLI 2009b, pp. 1-2). The Junin National Reserve has an area of 133,437 ac (53,000 ha), bordering Lake Junin and its adjacent territories (Wege and Long 1995, p. 264). In Peru, National Reserves are also created for the sustainable extraction of certain biological resources (BLI 2008, p. 1; Rodriguez and Young 2000, p. 330). Established in 1974, through Supreme Decree No. 0750-74-AG, the stated objectives of the Junin National Reserve include: integrated conservation of the local ecosystem, its associated flora and wildlife; preservation of the scenic beauty of the lake; and support of socioeconomic development in the area through the sustainable use of its renewable natural resources (BLI 2009a, p. 2; Hirshfeld 2007, p. 107). Most of the lakeshore is designated a ``Direct Use Zone,'' which allows fishing, grazing, and other educational, research, and recreational activities (ParksWatch 2006, p. 12). Although designation of this reserve has heightened awareness of the ecological problems at Lake Junin (BLI 2009c, p. 1), it has not reduced or eliminated the primary threats to the Junin grebe: water fluctuations and contamination (Factor A), contamination resulting in poor health (Factor C), and small population size (Factor E). Therefore, the existence of this species within a protected area has not reduced or mitigated the threats to the species. The Junin National Reserve was designated a Ramsar site under the Convention on Wetlands of International Importance (Ramsar Convention) in 1997 (BLI 2009a, p. 2; Hirshfeld 2007, p. 107; INRENA 1996, pp. 1- 14). The Ramsar Convention, signed in Ramsar, Iran, in 1971, is an intergovernmental treaty which provides the framework for national action and international cooperation for the conservation and wise use of wetlands and their resources. There are presently 159 Contracting Parties to the Convention (including Lake Junin), with 1,874 wetland sites, totaling more than 457 million ac (185 million ha), designated for inclusion in the Ramsar List of Wetlands of International Importance (Ramsar 2009, p. 1). Peru acceded to Ramsar in 1992. It has 13 sites on the Ramsar list, comprising 16.8 million ac (6.8 million ha) (Ramsar 2009, p. 5). In an examination of 5 Ramsar sites, experts noted that Ramsar designation may provide nominal protection (protection in name only) by increasing both international awareness of a site's ecological value and stakeholder involvement in conservation (Jellison et al. 2004, pp. 1, 4, 19). However, activities that negatively impact the Junin grebe are [[Page 621]] ongoing within this Ramsar wetland, including water fluctuations and contamination (Factor A), contamination resulting in poor health (Factor C), and small population size (Factor E). Therefore, the Ramsar designation has not mitigated the impact of threats on the Junin grebe. In 2002, the Peruvian Government passed an emergency law to protect Lake Junin. This law makes provisions for the cleanup of Lake Junin, and places greater restrictions on extraction of water for hydropower and mining activities (J. Fjeldsa in litt. 2003, as cited in BLI 2007, p. 3). However, this law has not been effectively implemented, and conditions around the lake may even have worsened after passage of this law (BLI 2009c, p. 1). The Ministry of Energy and Mining has implemented a series of Environmental Mitigation Programs (PAMA) to combat mine waste pollution in the Junin National Reserve (ParksWatch 2006, p. 21; ParksWatch 2009 p. 3). The PAMAs were scheduled to have been completed by 2002, but extensions have been granted, indicating that many of the mines currently in operation are still functioning without a valid PAMA. Reductions in pollution are reported because some mine companies have begun to utilize drainage fields and recycle residual water. However, analysis of existing PAMAs indicate that they do not address specific responsibilities for mining waste discharged into the Rio San Juan and delta, nor do they address deposition of heavy metal-laced sediments in Lake Junin (ParksWatch 2006, p. 21; ParksWatch 2009, p. 3). Recent information indicates that mining waste contamination in the lake continues to be a source of pollution (ParksWatch 2006, pp. 20-21; Fjeldsa 2004, p. 124; BLI 2009b, p. 1). Therefore, this law is not effective at mitigating the threat of habitat degradation (Factor A), health issues associated with contamination (Factor C) and small population size of the species (Factor E). There are approximately 5,000 laws and regulations directly or indirectly related to environmental protection and natural resource conservation in Peru. Recent studies by the Peruvian Society for Environmental Law (SPDA) have concluded that many of these are not effective because of limited implementation and/or enforcement capability (Muller 2001, pp. 1-2). Summary of Factor D Peru has enacted various laws and regulatory mechanisms for the protection and management of wildlife and their habitats. The Junin grebe is ``critically endangered'' under Peruvian law, and its entire population occurs within one protected area. As discussed under Factor A, the Junin grebe's distribution, breeding success and recruitment, and food availability on Lake Junin has been curtailed, and are negatively impacted due to habitat destruction that is caused by artificial water fluctuations and water contamination from human activities. These habitat-altering activities are ongoing throughout the species' range, which is wholly encompassed within one protected area. Thus, despite the species' critically endangered status and presence within a designated protected area, laws governing wildlife and habitat protection in Peru are inadequately enforced or ineffective at protecting the species or mitigating ongoing habitat degradation (Factor A), impacts from contaminants, and concomitant population declines (Factor E). Therefore, we find that the existing regulatory mechanisms are inadequate to mitigate the threats to the continued existence of the Junin grebe throughout its range. E. Other Natural or Manmade Factors Affecting the Continued Existence of the Species An additional factor that affects the continued existence of the Junin grebe is the species' small population size. The current population of the Junin grebe is estimated to be 100-300 individuals, however, only an ``extremely small number of adults remain'' (BLI 2008, p. 1; BLI 2009b, pp. 1, 3). The number of adults in a population are important because these individuals contribute to the next generation (Shaffer 1981, pp. 132-133; Soule 1980, pp. 160-162). The Junin grebe underwent a severe population decline in the latter half of the 20th century, with extreme population fluctuations (Fjeldsa 1981, p. 254) (see Population Estimates). For example, in 1993, the population size declined to below 50 individuals, of which fewer than half were breeding adults (BLI 2008, p. 3; BLI 2009b, p. 2). Even if the estimate of 100-300 individuals is correct, the number of mature individuals is likely to be far smaller, perhaps only half (Fjeldsa in litt. 2003, as cited in BLI 2009b, p. 2). Therefore, 100-300 individuals overestimates the species' effective population size (the number of breeding individuals that contribute to the next generation) (Shaffer 1981, pp. 132-133; Soule 1980, pp. 160-162). Small population size renders species vulnerable to genetic risks that can have individual or population-level genetic consequences, such as inbreeding depression, loss of genetic variation, and accumulation of new mutations, and may affect the species' viability by increasing its susceptibility to demographic shifts or environmental fluctuations, as explained in more detail above in the Factor E analysis for the ash- breasted tit-tyrant (Charlesworth and Charlesworth 1987, p. 238; Pimm et al. 1988, pp. 757, 773-775; Shaffer 1981, p. 131). Small population size also leads to a higher risk of extinction and, once a population is reduced below a certain number of individuals, it tends to rapidly decline towards extinction (Frankham 1996, p. 1507; Franklin 1980, pp. 147-148; Gilpin and Soule 1986, p. 25; Holsinger 2000, pp. 64-65; Purvis et al. 2000, p. 1949; Reed and Frankham 2003, pp. 233-234; Soule 1987, p. 181). In addition, species that inhabit a small geographic range, occur at low density, occupy a high trophic level, and exhibit low reproductive rates tend to have a higher risk of extinction than species that are not limited by the same risk factors (Purvis et al. 2000, p. 1949). Complications arising from the species' small population size are exacerbated by the species' restricted range and threat of disease (Factor C). The Junin grebe is known only from a single Andean lake, Lake Junin, in central Peru (BLI 2000, p. 45; BLI 2009b, p. 1; Collar et al. 1992, p. 43). Although the species was believed to have been previously distributed throughout the entire 57-mi\2\ (147-km\2\) lake (Fjeldsa 1981, p. 254; F. Gill and R.W. Storer, pers. comm. as cited in Fjeldsa 2004, p. 200; Morrison 1939, p. 645), it is now restricted to the southern portion of Lake Junin (BLI 2009b, p. 1; Fjeldsa 1981, p. 254; F. Gill and R.W. Storer, pers. comm. as cited in Fjeldsa 2004, p. 200). The population has declined by at least 14 percent in the last 10 years and is expected to continue to decline, as a result of declining water quality and extreme water level fluctuations (BLI 2009b, pp. 1, 4, 6-7) (Factor A). We consider that the risks associated with small population size will continue to impact this species and may accelerate, if habitat destruction continues unabated. Environmental contamination poses direct and indirect threats to the Junin grebe's overall health and survival, including the presence of toxins in both the Junin grebe and its primary prey species and mass die-offs that are linked to contamination or reduction in prey species (Factor C). A species' small population size, combined with its restricted range and threat of disease, increases the species' [[Page 622]] vulnerability to adverse natural events and manmade activities that destroy individuals and their habitat (Holsinger 2000, pp. 64-65; Primack 1998, pp. 279-308; Young and Clarke 2000, pp. 361-366). Summary of Factor E The Junin grebe has a small population size that renders it vulnerable to genetic risks that negatively impact the species' long- term viability and, possibly, its short-term viability. The species has a restricted range and occurs in habitat that continues to undergo degradation and curtailment due to human activities (Factor A). Environmental contaminants have caused die-offs of the species and have likely reduced the overall general health of the Junin grebe population (Factor C). The small population size, as well as its restricted range and health issues associated with contamination, increases the species' vulnerability to extinction, through demographic or environmental fluctuations. Based on its small population size, restricted range, and threat of disease, we have determined that the Junin grebe is particularly vulnerable to the threat of adverse natural events (e.g., genetic, demographic, or environmental) and human activities (e.g., water level manipulation) that destroy individuals and their habitat. The genetic and demographic risks associated with small population sizes are exacerbated by ongoing human activities that continue to curtail the species' habitat throughout its range. The species' population has declined and is predicted to continue declining due to an ongoing reduction in water quality and extreme water level fluctuations (Factor A). Therefore, we find that the species' small population size, in concert with its restricted range, threat of disease, and its heightened vulnerability to adverse natural events and manmade activities, are threats to the continued existence of the Junin grebe throughout its range. Status Determination for the Junin Grebe The Junin grebe, a flightless grebe, is endemic to Lake Junin, found at 13,390 ft (4,080 m) above sea level in Peru, where it resides year-round. The species relies on the open waters and marshland margins of the lake for feeding and on the protective cover of the marshland margins for nesting during the breeding season. The species has a highly restricted range (approximately 55 mi\2\ (143 km\2\)) and is currently known only in one location in central Peru. The species' population size is estimated as 100-300 individuals, although the number of mature individuals may be limited to half this amount. We have carefully assessed the best available scientific and commercial information regarding the past, present, and potential future threats faced by the Junin grebe and have concluded that there are four primary factors that threaten the continued existence of the Junin grebe: (1) Habitat destruction, fragmentation, and degradation; (2) disease; (3) limited size and isolation of remaining populations; and (4) inadequate regulatory mechanisms. Human activities that degrade, alter, and destroy habitat are ongoing throughout the Junin grebe's range. Lake waters are artificially manipulated to produce hydropower, resulting in reductions in water levels that impact the species' nesting and foraging sites. Manipulation of water levels for hydropower production reduces prey populations, causes increased food competition with white-tufted grebes, and results in the abandonment of breeding in drought years. Reduced water levels have permanently destroyed segments of giant bulrush communities, compromising the amount of suitable flooded marshland available for nesting (Factor A). Mining, agricultural, and organic materials have contaminated the water, causing eutrophication and anoxia in portions of the lake and the accumulation of trace minerals in lake bottom sediments. This has had direct effects on the Junin grebe, destroying habitat in the northwest portion of the lake so that the species' range is restricted to only the southern portion of Lake Junin and causing Junin grebe mortality during times of drought (Factors A and C). Contaminants have also reduced or eliminated submerged and emergent vegetation throughout the lake, decreasing pupfish spawning habitat and reducing prey availability (Factor A). Junin grebe habitat continues to be altered by human activities, conversion, and destruction of habitat, which reduce the quantity, quality, distribution, and regeneration of habitat available for the Junin grebe on Lake Junin. Habitat loss was a factor in the Junin grebe's historical population decline (see Historical Range and Distribution). Population declines have been correlated with water availability, and droughts have caused severe population fluctuations that have likely compromised the species' long-term viability. The Junin grebe population is small, rendering the species vulnerable to the threat of adverse natural (e.g., genetic, demographic) and human activity (e.g., water extraction and contaminants from mining) events that destroy individuals and their habitat. (Factor E). The Junin grebe's reproductive success and life cycle relies on the availability of sufficient water in Lake Junin. During drought years, nesting and reproduction decline. Although the population appears to rebound during wetter years (such as following excessive rains from El Ni[ntilde]o events (see Population Estimate and Factor A), excessive rains also bring additional contaminants into the lake as runoff from lands surrounding the lake and upstream rivers. Research indicates that both the Junin grebe and its primary prey species, the pupfish, have accumulated toxins resulting in elevated lead levels. Environmental contaminants have caused die-offs of the species and have likely reduced the general health of the Junin grebe population (Factor C). The population has declined 14 percent in the past 10 years (see Population Estimates), and this decline is predicted to continue commensurate with ongoing threats from habitat destruction and water contamination (Factor A). Despite the species' ``critically endangered'' status in Peru and its occurrence entirely within a protected area, the lake continues to be destroyed and degraded as a result of human activities that alter the lake's water levels and compromise water quality (Factors A and C). Therefore, regulatory mechanisms are either inadequate or ineffective at mitigating the existing threats to the Junin grebe and its habitat (Factor D). Section 3 of the Act defines an ``endangered species'' as ``any species which is in danger of extinction throughout all or a significant portion of its range'' and a ``threatened species'' as ``any species which is likely to become an endangered species within the foreseeable future throughout all or a significant portion of its range.'' Based on the immediate and ongoing threats to the Junin grebe throughout its entire range, as described above, we determine that the Junin grebe is in danger of extinction throughout all of its range. Therefore, on the basis of the best available scientific and commercial information, we are proposing to list the Junin grebe as an endangered species throughout all of its range. III. Junin rail (Laterallus tuerosi) Species Description The Junin rail is a secretive bird of the Rallidae family that is endemic to a single lake (Lake Junin) in Peru. The species is also referred to as the Junin black rail (Fjeldsa 1983, p. 281) and is locally known as ``gallinetita de Junin'' [[Page 623]] (Supreme Decree 034-2004-AG 2004, p. 27684). This rail measures 4.7-5.1 in (12-13 cm) in length, and has a dark slate-colored head, throat and underparts. Its belly and vent (anal aperture) are black, with heavily- barred white primary feathers along the back and side. The under-tail coverts (feathers on the underside of the base of the tail) are buff in color, with a dull rufous-brown back. The remaining underparts are dark brown and boldly barred in white, and the legs are greenish-yellow (BLI 2009b, p. 1). Taxonomy The Junin rail was first taxonomically described as Laterallus spilonotus after Gould's 1841 description of the Galapagos crake (Fjeldsa 1983, p. 281; ITIS 2009b, p. 1). Fjeldsa (1983) later described it as a subspecies of the black rail (Laterallus jamaicensis tuerosi) (Fjeldsa 1983, pp. 277-282; Fjeldsa and Krabbe 1990, p. 146; ITIS 2009a, p. 1), noting that the bird's morphology may suggest that it is a distinct species (Fjeldsa 1983, p. 281). Laterallus jamaicensis has since been split into L. jamaicensis and L. tuerosi (BLI 2009b, p. 1). ITIS continues to list the species as the subspecies L. j. tuerosi based on Fjeldsa's work in 1983 (ITIS 2009a, p. 1). BirdLife International considers this rail a full species based on morphological differences (BLI 2009b p. 1). Furthermore, the black rail, Laterallus jamaicensis occurs at much lower elevations (i.e., 0 to 4,429 ft (0 to 1,350 m) above sea level) (Collar et al. 1992, p. 190; BLI 2000, p. 170; BLI 2007, p. 1). Based on the morphological differences and the species' distinct and disjunct ranges, we consider the Junin rail to be a discrete species and recognize it as L. tuerosi. It should be noted that it appears that only 2 specimens of the Junin rail have ever been collected (near Ondores) (Fjeldsa 1983, pp. 278-279) and that all expert accounts of this species rely solely on that collection and a subsequent observation of the species in Pari (Fjeldsa in litt., 1992, as cited in Collar et al. 1992, p. 190). Habitat and Life History The Junin rail inhabits Lake Junin at 13,390 ft (4,080 m) above sea level in the Andean highlands of Peru (Junin Region). The Junin rail occurs in the dense, interior marshlands where rushes (Juncus spp.) predominate or in more open mosaics of rushes, mosses (division Bryophyta), and low herbs (Fjeldsa 1983, p. 281). Lake Junin, home to the Junin grebe that is also the subject of this proposed rule, is located in the seasonally climatic ``puna'' habitat, with a variety of species of grasses, asters, and trees of the bean family forming tall, dense grasslands and open scrubland, interspersed with wetlands and woodlands (ParksWatch 2006, p. 2; ParksWatch 2009, pp. 1, 4). Giant bulrushes and totorilla dominate the extensive marshlands surrounding the lake (BLI 2009b, p. 1; Fjeldsa 1983, p. 281; ParksWatch 2009, p. 1). In shallow water, during low lake levels, ``tortora'' communities can become partially or completely dry (ParksWatch 2009, p. 2). The lake supports a wide variety of bird species and aquatic vegetation (BLI 2003, p. 1; BLI 2009a, pp. 2-3; ParksWatch 2009, p. 3; Tello 2007, p. 2). Mammals are relatively scarce in the area, although there are some predators (ParksWatch 2009, p. 4) (see Factor C). A more detailed discussion of the flora and fauna of the lake are provided above as part of the analysis of the Habitat and Life History of the Junin grebe. There is little information regarding the ecology of the Junin rail. The species appears to be completely dependent on the wide marshlands located around the southeast shoreline of the lake for nesting, foraging, and year-round residence (BLI 2009b, p. 2; Collar et al. 1992, p. 190; Fjeldsa 1983, p. 281) (see also Current Range and Distribution). Similar to all rails, the Junin rail is furtive and remains well-hidden in the marshes surrounding the lake (BLI 2009b, p.2). The Junin rail reportedly nests at the end of the dry season, in September and October. Nests are built on the ground within dense vegetative cover, and the species' clutch size is two eggs (BLI 2009b, p. 2; Collar et al. 1992, p. 190). The diet of the Junin rail has not been studied specifically, but other black rail species feed primarily on small aquatic and terrestrial invertebrates and seeds (Eddleman et al. 1994, p. 1). Historical Range and Distribution The Junin rail is endemic to Lake Junin (Fjeldsa 1983, p. 278; BLI 2009b, p. 2). The species may have been historically common in the rush-dominated marshlands surrounding the entire lake (Fjeldsa 1983, p. 281). In addition to the species' specific habitat preferences (see Current Range and Distribution), it is believed that the Junin rail is now restricted to the marshes at the southwest corner of the lake because of the high level of water contamination that flows into the northwest margins of the lake via the Rio San Juan (Martin and McNee 1999, p. 662). Current Range and Distribution The Junin rail is restricted to the southwest shore of the lake, and is currently known in only two localities (near the towns of Ondores and Pari) (BLI 2009b, p. 2; Collar et al. 1992, p. 190; Fjeldsa 1983, p. 281). The current estimated range of the species is 62 mi\2\ (160 km\2\) (BLI 2009b, p. 1). However, this is likely an overestimate of the species' actual range for several reasons. First, BirdLife International's definition of a species' range results in an overestimate of the actual range (see Current Range and Distribution of the ash-breasted tit-tyrant) (BLI 2000, pp. 22, 27). Second, the species' range was calculated based on the availability of presumed suitable habitat for the Junin rail. It has long been assumed that the rail potentially occupies the entire marshland area surrounding Lake Junin (Fjeldsa 1983, p. 281). The total marshland area has been estimated by BirdLife International to be 58 mi\2\ (150 km\2\) (BLI 2000, p. 170; BLI 2007, p. 1; BLI 2008, p. 3; BLI 2009b, p. 1). However, the species has never been confirmed outside the two known localities on the southwest shore of the lake. Moreover, a better understanding of the ``puna'' habitat, as well as the habitat specificity (the specific habitat needs) of other rail species, indicates that these may be the only two localities for this species. Despite the apparently uniform appearance of the ``puna,'' the habitat provides a complex mosaic of niches that leads to the patchy distribution of many bird species throughout the region, indicating that the species have specialized habitat requirements that are only satisfied locally (Fjeldsa and Krabbe 1990, p. 32). The species' distribution is highly localized around the lake. The Junin rail apparently prefers the dense, interior marshlands comprised primarily of rushes (Juncus spp.) and mosaics of rushes, mosses (division Bryophyta), and low herbs in more open marsh areas (Fjeldsa 1983, p. 281). High habitat specificity is consistent with related rail species. Studies of the closely related California black rail (Laterallus jamaicensis coturniculus) indicate that this species is a habitat specialist, whereby the emergent vegetation used for cover, water depth, and access to upland vegetation, are all important factors in the black rail's habitat use (Flores and Eddleman 1995, p. 362). Therefore, the Junin rail's actual range is clearly smaller than the figure that continues to be reported by BirdLife International since 2000 (BLI 2000, p. 170; BLI 2007, p. 1; BLI 2008, p. 3; BLI 2009b, p. 1). [[Page 624]] Population Estimates Rigorous population estimates have not been made (Fjeldsa 1983, p. 281), and the species' elusiveness makes it difficult to locate (BLI 2009b, p. 2). In 1983, the Junin rail was characterized as possibly common, based on local fishers' sightings of groups of up to a dozen birds at a time (Fjeldsa 1983, p. 281). The species continues to be reported as ``fairly common,'' assuming that it occurs throughout the marshland surrounding the lake (BLI 2007, p. 1; BLI 2009b, p. 1). The BirdLife International estimate that this species' population size falls within the population range category of 1,000-2,499 (BLI 2000, p. 170; BLI 2007, p. 1; BLI 2009b, p. 1). This estimate is an extrapolation that continues to be based on the assumption that the species ``may be fairly common in the entire c. 58 mi\2\ (150 km\2\) of available marshland'' around Lake Junin (BLI 2000, p. 170; BLI 2007, p. 1; BLI 2008, p. 3; BLI 2009b, p. 1). As indicated in the analysis of this species' Current Range and Distribution, the species has never been confirmed outside its two known localities and, therefore, it is possible that the species is locally common, but not widely distributed. If the Junin rail is not common throughout Lake Junin's marshland, the actual population size may be much lower. The species has experienced a population decline of between 10 and 19 percent in the past 10 years (BLI 2009b, p. 2). The population is considered to be declining in close association with continued habitat loss and degradation (see Factors A, C, and E) (BLI 2008, p. 1). Conservation Status The Junin rail is considered ``endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276855). The IUCN categorizes the Junin rail as ``Endangered'' because it is known only from a small area of marshland (i.e., near Ondores and Pari) around a single lake, where habitat quality is declining (BLI 2008, p. 3). The single known population of the Junin rail occurs wholly within one protected area in Peru, the Junin National Reserve (BLI 2008, p. 1; BLI 2009b, pp. 1-2). Summary of Factors Affecting the Junin Rail A. The Present or Threatened Destruction, Modification, or Curtailment of the Species' Habitat or Range The Junin rail is endemic to Lake Junin, where it resides year- round and is restricted to two localities within the shallow marshlands encircling Lake Junin (BLI 2008, p. 3; BLI 2009b, p. 2; Fjeldsa 1983, p. 278). The current estimated range of the species, 62 mi\2\ (160 km\2\) (BLI 2009b, p. 1), is an overestimate of this species' range for the reasons outlined above as part of the analysis of this species' Current Range and Distribution. The species is known only from two discrete locations, near Ondores and Pari, on the southwest shore of the lake. Breeding occurs near the end of the dry season, in September and October, and the birds build their nests on the ground within the dense vegetative cover of the rushes that make up the marshland perimeter of the lake (BLI 2000, p. 170; BLI 2007, p. 1; BLI 2009b, p. 2). The habitat in and around Lake Junin is subjected to manmade activities that have altered, destroyed, and degraded the quantity and quality of habitat available to the Junin rail. These activities include: (1) artificial manipulation of water levels; (2) water contamination; and (3) plant harvesting in the species' breeding grounds. The negative impacts of these activities are accentuated by unpredictable climate fluctuations (such as droughts or excessive rains) (Jetz et al. 2007, pp. 1211, 1213; Mora et al. 2007, p. 1027). The Upamayo Dam went into operation at the northwest end of Lake Junin in 1936 to generate electricity using hydropower (Martin et al. 2001, p. 178; ParksWatch 2006, p. 5). Under normal conditions, water levels are lower during the dry season, and the marshlands can become partially or completely desiccated (BLI 2009b, p. 1; ParksWatch 2009, p. 2). The dam is often opened during the dry season, to generate power (June to November) (BLI 2009b, p. 1; ParksWatch 2009, p. 2), leading to further drawdown of the lake. Lake drawdown has been known to cause water levels to fluctuate seasonally up to 6 ft (2 m) (Martin and McNee 1999, p. 659) and has at times caused complete desiccation of the marshlands by the end of the dry season (Fjeldsa 2004, p. 123). The ground nesting Junin rail breeds near the end of the dry season, in September and October, and the species' relies on the dense vegetative cover of the rushes on the lake perimeter in which to build their nests (BLI 2009b, p. 2). A similar species, the California black rail, may tolerate decreases in water depth, but only if the substrate remains moist enough to support sufficient wetland vegetation (Flores and Eddleman 1995, p. 362). Eddleman et al. (1988, p. 463) noted that water drawdown before nesting season disrupts nest initiation by rails. Therefore, water drawdown near the end of the dry season that results in complete desiccation of the shallow marshlands (BLI 2009b, p. 1; ParksWatch 2009, p. 2) is likely to disrupt Junin rail nest initiation. Experts believe that the Junin rail is restricted to the marshes at the southwest corner of the lake because of the high level of contamination at the northwest margins of the lake (Martin and McNee 1999, p. 662). Experts also believe that pollution and artificial water level fluctuations will continue to have adverse consequences for the vegetation surrounding the lake and, therefore, the Junin rail (BLI 2000, p. 170; BLI 2007, p. 1; J. Fjeldsa in litt., 1987, as cited in Collar et al. 1992, p. 190). Indeed, in some places, the tall marshlands, which rely on inundated soils to thrive, have virtually disappeared because the reed-beds are no longer permanently inundated (O'Donnel and Fjeldsa 1997, p. 30). Moreover, as the marshes dry, livestock (primarily sheep (Ovis aries), but also cattle (Bos taurus), and some llamas (Llama glama) and alpacas (Llama pacos)) move into the desiccated wetlands surrounding the lake to graze. Overgrazing is a year-round problem around Lake Junin because the entire lakeshore is zoned for grazing by a large number of livestock (approximately 60,000- 70,000 head) (ParksWatch 2006, pp. 12, 19). During the dry season, the hoofed stock moves into the marshlands to graze, compacting the soil and trampling the vegetation (ParksWatch 2006, p. 31). Increased access to the wetlands during the end of the dry season, which coincides with the inception of the Junin rail's nesting season, likely disrupts the rail's nesting activities or leads to nest trampling. Therefore, activities that increase lakeshore access, such as water drawdown, decrease the amount of available habitat for the Junin rail (for nesting and feeding) and are likely to negatively impact the Junin rail's reproduction (through trampling) and mating habits (through disturbance) (BLI 2009b, p. 1). Water quality is another factor influencing the quality of habitat available to the Junin rail. The degraded water quality in Lake Junin was fully discussed as part of the Factor A analysis for the Junin grebe and is summarized here. The water in Lake Junin has been contaminated from mining (Martin and McNee 1999, pp. 660-661; ParksWatch 2006, p. 2; Shoobridge 2006, p. 3), agricultural activities (Martin and McNee 1999, pp. 660-661; ParksWatch 2006, p. 2; [[Page 625]] Shoobridge 2006, p. 3), and from organic matter and wastewater runoff from local communities around the lake (ParksWatch 2006, pp. 5, 19; Shoobridge 2006, p. 3). Water pollution has resulted in heavy metal contamination throughout the lake, exceeding established thresholds for aquatic life throughout at least one-third of the lake (Martin and McNee 1999, pp. 660-661; O'Donnel and Fjeldsa 1997, p. 29; ParksWatch 2006, pp. 3, 20; Shoobridge 2006, p. 3), and rendering the northern portion of the lake lifeless (BLI 2008, p. 4; Shoobridge 2006, p. 3; Fjeldsa 2004, p. 124; Martin and McNee 1999, pp. 660-662; ParksWatch 2006, pp. 20-21). At the lake's center, lake bottom sediments are lifeless and anoxic due to contaminants (Fjeldsa 2004, p. 124; Martin et al. 2001, p. 180), and the lakeshore has become polluted with ``toxic acid gray sediment'' (O'Donnel and Fjeldsa 1997, p. 30). There is no vegetation at the northern end of the lake (Fjeldsa 2004, p. 124; ParksWatch 2006, pp. 20-21), and ongoing contamination has the potential to reduce vegetative cover in other areas of the lake, including the marshlands where the Junin rail occurs. In addition, these pollutants have severely affected animal and plant populations in the area, contributing to mortality of species around the lake (ParksWatch 2006, pp. 3, 20) and have the potential to reduce the health and fitness of the Junin rail (see Factor C). Local residents also harvest and burn cattails from the marshland habitat, which the Junin rail depends upon. Cattails are harvested for use in construction (i.e., to assemble rafts, baskets, and mats) and as forage for livestock (ParksWatch 2006, p. 23). Cattails are also burned to encourage shoot renewal (ParksWatch 2006, p. 23) and for hunting the montane guinea pig (Cavia tschudii), which seeks cover in the cattail marshes and is part of the local diet. Burning cattail communities has a negative and long-lasting impact on species that use the cattails as permanent habitat (INRENA 2000, as cited in ParksWatch 2006, p. 22; Eddleman et al. 1988, p. 464), including the Junin rail, which relies on the dense vegetative cover of the marshlands for year-round residence and nesting (BLI 2000, p. 170; BLI 2007, p. 1; BLI 2009b, p. 2). The negative impacts of habitat alteration and destruction (such as artificially reduced water levels, water contamination, and cattail harvesting and burning) are accentuated by unpredictable climate fluctuations (such as droughts or excessive rains) (Jetz et al. 2007, pp. 1211, 1213; Mora et al. 2007, p. 1027). Peru is subject to unpredictable climate fluctuations, such as those that are related to the El Ni[ntilde]o Southern Oscillation (ENSO). Changes in weather patterns, such as ENSO cycles (El Ni[ntilde]o and La Ni[ntilde]a events), tend to increase precipitation in normally dry areas, and decrease precipitation in normally wet areas (Holmgren et al. 2001, p. 89; TAO Project n.d., p. 1). ENSO events exacerbate the effects of habitat reduction and alteration on the decline of a species (England 2000, p. 86; Holmgren et al. 2001, p. 89; Jetz et al. 2007, pp. 1211, 1213; Mora et al. 2007, p. 1027; Parmesan and Mathews 2005, p. 334; Plumart 2007, pp. 1-2; Timmermann 1999, p. 694), particularly for narrow endemics (Jetz et al. 2007, p. 1213) such as the Junin rail (see also Factor E). As discussed above, droughts increase access to the wetlands where Junin rails live and breed. Excessive rain increases contamination in the water and causes soil toxicity (see Factor C). ENSO cycles are ongoing, having occurred several times within the last decade (NWS 2009, p. 2). Evidence suggests that ENSO cycles have already increased in periodicity and severity (Richter 2005, pp. 24-25; Timmermann 1999, p. 694), which will, thus, exacerbate the negative impacts on a species. Summary of Factor A The habitat in and around Lake Junin, where the Junin rail is endemic, has been and continues to be altered and degraded as a result of human activities, including artificial water level fluctuations to generate hydropower, water contamination caused by mining waste, agricultural and organic runoff from surrounding lands, and wastewater from local area communities. The Junin rail is dependent on the marshland habitat surrounding the lake for breeding and feeding. Water levels in Lake Junin are manipulated to generate electricity, which leads to dramatic fluctuations in water levels of up to 6 ft (1.8 m). The Junin rail nests on the ground, within the protective cover of the marshlands. As water drawdown occurs near the end of the dry season and during the inception of the Junin rail's mating season, portions of the marshlands may dry out completely, affecting the availability of suitable breeding and foraging habitat. This species' population decline has been linked to deteriorating habitat quality (see also Factor E). Overgrazing, cattail harvest, and burning are ongoing around the lakeshore, and water drawdown increases access to the marshlands. Severe water contamination in the northwest portion of the lake has rendered it lifeless, and experts believe that water contamination limits the Junin rail's foraging and breeding activities to the southern portion of the lake. The effects of artificially reduced water levels and water contamination are accentuated by droughts or excessive rains caused by El Ni[ntilde]o events. Reduced water levels near the end of the dry season (during Junin rail nesting season) expose the species to greater vulnerability to predation (see Factor C), which also heightens the risks to the species that are associated with short- and long-term genetic viability (Factor E). Therefore, we find that destruction and modification of habitat are threats to the continued existence of the Junin rail throughout its range. B. Overutilization for Commercial, Recreational, Scientific, or Educational Purposes We are not aware of any information currently available that indicates that overutilization of Junin rail for commercial, recreation, scientific, or education purposes has occurred or is occurring at this time. As a result, we are not considering overutilization to be a threat to the continued existence of the Junin rail. C. Disease or Predation Disease: Although no specific disease threat has been identified for the Junin rail, contamination of Lake Junin exposes the Junin rail to mortality and a reduction the overall fitness and health of the species. The effects of water contamination on the health of species inhabiting Lake Junin were discussed as part of the analysis of Factor C for the Junin grebe and are summarized here. In Lake Junin, mining activities (Martin and McNee 1999, pp. 660-661; Shoobridge 2006, p. 3), and agricultural runoff, organic matter, and wastewater (ParksWatch 2006, pp. 5, 19; Shoobridge 2006, p. 3) have contaminated the entire lake with high concentrations of dissolved chemicals (Fjeldsa 2004, p. 124; Martin and McNee 1999, pp. 660-662; ParksWatch 2006, pp. 20-21). Environmental contaminants exceed current established thresholds for aquatic life (Martin and McNee 1999, pp. 660-661; ParksWatch 2006, p. 20) and have rendered the northern portion of the lake lifeless from eutrophication (BLI 2008, p. 4; Shoobridge 2006, p. 3). Due to severe contamination, the sediments in the center of the lake are anoxic (Martin et al. 2001, p. 180), and the lake's turbidity has increased (ParksWatch 2006, p. 20). Chemical waste has damaged at least one third of the lake, severely affecting animal and [[Page 626]] plant populations in the area (O'Donnel and Fjeldsa 1997, p. 29; ParksWatch 2006, pp. 3, 20; Shoobridge 2006, p. 3) and completely eliminating vegetation from the northern portion of the lake (Fjeldsa 2004, p. 124; ParksWatch 2006, pp. 20-21). It is also believed that contamination may, in fact, be responsible forthe possible extirpation of at least one fish species (a catfish) (O'Donnel and Fjeldsa 1997, p. 29). Contamination from mining waste may have direct and indirect impacts on the fitness and health of the Junin rail. As described above as part of the Factor C analysis for the Junin grebe, a waterbird that is sympatric with the Junin rail, mine waste contamination may have caused heavy metal poisoning (T. Valqui and J. Barrio in litt. 1992, as cited in Collar et al. 1992, pp. 45, 190) or reduced prey availability (Fjeldsa 2004, p. 124), leading to Junin grebe mortality during an extensive drought from 1989 to 1992. Large-scale bird mortality has occurred on the lake, apparently due to lead poisoning from mining effluents--a common cause of mortality in waterbirds (Friend and Franson 1999, p. 317; O'Donnel and Fjeldsa 1997, p. 30). Heavy metals in the water column and the lake's sediments, where this species feeds, would have negative health consequences for the Junin rail, as in the case for the Junin grebe and other waterbirds that inhabit the lake. Excessive contaminant load can contribute to a decline in fitness and vigor for long-lived, mid-trophic level species (Rowe 2008, p. 624), such as the Junin rail. Increased turbidity of the water has resulted in die-offs of aquatic plants and algae, which disrupts the food chain (ParksWatch 2006, p. 20). Higher trophic level species (discussed in more detail as part of the Factor C analysis for the Junin grebe), such as the Junin rail, are more susceptible to disruptions in the food chain at lower trophic levels (Fjeldsa 2004, p. 123; The University of the Western Cape 2009, p. 1) and prone to bioaccumulation because they ingest pesticides, heavy metals, and other contaminants that are present in their prey (Demayo et al. 1982, as cited in Eisler 1988, p. 5; Fjeldsa 2004, p. 123). Drought conditions exacerbate the effects of water contamination and bioaccumulation for species at higher trophic levels (Demayo et al. 1982, as cited in Eisler 1988, p. 5; Fjeldsa 2004, p. 123). Predation: Predators around Lake Junin include the Andean fox (Pseudalopex culpaues), the long-tailed weasel (Mustela frenata), Pampas cat (Onicifelis colocolo), and hog-nosed skunk (Conepatus chinga) (ParksWatch 2009, p. 4). Junin rails are preyed upon by pampas cats (BLI 2008, p. 4; BLI 2009b, p 2). Under normal conditions, water levels are lower in the dry season and the marshlands can become partially or completely dry (BLI 2009b, p. 1; ParksWatch 2009, p. 2) reducing protective cover and allowing predators to more easily locate the rail. When the floodgates of the Upumayo Dam are opened during the dry season (June to November) (BLI 2009b, p. 1; ParksWatch 2009, p. 2), drawdown has led to complete desiccation of the marshlands by the end of the dry season (Fjeldsa 2004, p. 123). The ground nesting Junin rail breeds near the end of the dry season, in September and October, and builds their nests in the dense vegetative cover of the rushes on the lake perimeter (BLI 2009b, p. 2). Water drawdown and periods of drought increases the bird's vulnerability to predation because nesting grounds become exposed and larger areas of the marsh are accessible to predators (ParksWatch 2006, p. 23). Predation increases the risk of extirpation due to the species' already small population size. In addition, species that inhabit a small geographic range, occur at low density, occupy a high trophic level, and exhibit low reproductive rates tend to have a higher risk of extinction than species that are not limited by the same risk factors (Purvis et al. 2000, p. 1949) (Factor E). Summary of Factor C Environmental contaminants (Factor A) in Lake Junin may have negative consequences on the health of the Junin rail, given that extensive environmental contamination in Lake Junin has resulted in mortality of flora and fauna that inhabit the lake and its margins. The species' trophic level also exposes it to bioaccumulation of toxins accumulated in the tissue of prey species. There is documented evidence that other waterbirds occupying the same habitat have died as a direct result of contaminant poisoning or reduction of the availability of prey species. Therefore, we find that disease is a threat to the continued existence of the Junin rail. Predation by the pampas cat results in the direct removal of individuals from the population and can remove potentially reproductive adults from the breeding pool. Ongoing habitat destruction (through reduced water levels and contamination) continues to degrade the quality of habitat available to the Junin rail (Factor A) and the species' habitat becomes more accessible to predators during droughts and water drawdowns. Predation renders the species particularly vulnerable to local extirpation due to its small population size (Factor E). Therefore, we find that predation, exacerbated by ongoing habitat destruction (Factor A), are threats to the continued existence of the Junin rail throughout its range. D. Inadequacy of Existing Regulatory Mechanisms The Junin rail is listed as ``endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276855). This Decree prohibits hunting, take, transport, and trade of protected species, except as permitted by regulation. As hunting, take, transport, and trade do not currently threaten the Junin rail, this regulation does not mitigate any current threats to this species. Peru has several categories of national habitat protection, which were described above as part of the Factor D analysis for the ash- breasted tit-tyrant (BLI 2008, p. 1; IUCN 1994, p. 2; Rodriguez and Young 2000, p. 330). The single Junin rail population occurs wholly within the Junin National Reserve (Junin, Peru) (BLI 2009b, pp. 1-2), which encompasses the lake and surrounding land and was established in 1974 by Supreme Decree 0750- 74-AG (BLI 2009a, p. 2; Wege and Long, p. 264). Peruvian National Reserves are created for the sustainable extraction of certain biological resources (BLI 2008, p. 1; Rodriguez and Young 2000, p. 330), and most of the lakeshore is designated a ``Direct Use Zone,'' allowing fishing, grazing, and other educational, research, and recreational activities (ParksWatch 2006, p. 12). Habitat destruction and alteration (through artificial water level fluctuations, contamination (BLI 2009b, p. 1; Fjeldsa 2004, p. 124; ParksWatch 2006, pp. 20-21; Wege and Long 1995, p. 264)), overgrazing, and cattail harvest and burning (ParksWatch 2006, pp. 22-23) are ongoing throughout the Reserve (Factor A), increasing the species' susceptibility to predation (ParksWatch 2006, p. 23) (Factor C), and jeopardizing the continued existence of the species, given its already small population size (Factor E). Therefore, the existence of this species within a protected area has not reduced or mitigated the threats to the Junin rail. The Junin National Reserve was designated a Ramsar site in 1997 (BLI 2009a, p. 2; INRENA 1996, pp. 1-14; Ramsar 2009, p. 2). As more fully described for the Junin grebe, this designation provides only nominal protection of wetland habitat (Jellison et [[Page 627]] al. 2004, p. 19). Activities that negatively impact the Junin rail are ongoing throughout this wetland, including water fluctuations and contamination (Factor A), water fluctuations that increase the species' risk of predation (Factor C), and small population size (Factor E). Therefore, the Ramsar designation has not mitigated the impact of threats on the Junin rail. There are approximately 5,000 laws and regulations directly or indirectly related to environmental protection and natural resource conservation in Peru. Recent studies by the Peruvian Society for Environmental Law (SPDA) have concluded that many of these are not effective because of limited implementation or enforcement capability (Muller 2001, pp. 1-2). Summary of Factor D Peru has enacted various laws and regulatory mechanisms for the protection and management of wildlife and their habitats. The Junin rail is ``endangered'' under Peruvian law, and its entire population occurs within a protected area. As discussed under Factor A, habitat destruction and alteration have curtailed the species' range and threaten the continued existence of the species. Ongoing habitat destruction (including water level manipulation, contamination, overgrazing, and cattail harvest and burning (Factor A)), predation (Factor C), and predators' increased access due to habitat destruction intensify the risks to the species from its already small population size (Factor E). These activities are ongoing throughout the species' range, which is entirely encompassed within a protected area. Thus, despite its endangered status and its presence within a designated protected area, laws governing wildlife and habitat protection in Peru are inadequately enforced or ineffective at protecting the species or mitigating ongoing habitat degradation (Factor A) and concomitant population declines (Factor E). Therefore, we find that the existing regulatory mechanisms are inadequate to mitigate the threats to the continued existence of the Junin rail throughout its range. E. Other Natural or Manmade Factors Affecting the Continued Existence of the Species An additional factor that affects the continued existence of the Junin rail is the species' small population size. As discussed above (see Population Estimates), BirdLife International has placed the Junin rail in the population category of between 1,000 and 2,499 individuals (BLI 2009b, p. 2), and considers the population to be likely ``very small and presumably declining'' (BLI 2000, p. 170; BLI 2009b, p. 1). Small population size renders species vulnerable to genetic risks that can have individual or population-level genetic consequences, such as inbreeding depression, loss of genetic variation, and accumulation of new mutations, and may affect the species' viability by increasing its susceptibility to demographic shifts or environmental fluctuations, as explained in more detail above in the Factor E analysis for the ash- breasted tit-tyrant (Charlesworth and Charlesworth 1987, p. 238; Pimm et al. 1988, pp. 757, 773-775; Shaffer 1981, p. 131). Small population size leads to a higher risk of extinction and, once a population is reduced below a certain number of individuals, it tends to rapidly decline towards extinction (Frankham 1996, p. 1507; Franklin 1980, pp. 147-148; Gilpin and Soule 1986, p. 25; Holsinger 2000, pp. 64-65; Purvis et al. 2000, p. 1949; Reed and Frankham 2003, pp. 233-234; Soule 1987, p. 181). In addition, species that inhabit a small geographic range, occur at low density, occupy a high trophic level, and exhibit low reproductive rates tend to have a higher risk of extinction than species that are not limited by the same risk factors (Purvis et al. 2000, p. 1949). We consider that the risks associated with small population size will continue to impact this species and may accelerate, if habitat destruction continues unabated. Complications arising from the species' small population size are exacerbated by its restricted range and the threat of predation (Factor C). The Junin rail is known from two localities (Ondores and Pari) on the southwest shore of a single Andean lake, Lake Junin, in central Peru (BLI 2000, p. 170; BLI 2009b, pp. 1-2; Fjeldsa 1983, p. 281; Fjeldsa in litt. 1992, as cited in Collar et al. 1992, p. 190) (see Current Range and Distribution). The population has declined at a rate between 10 and 19 percent in the past 10 years, and this decline is expected to continue, as a result of the declining quality of habitat within its small, restricted range (BLI 2009b, pp. 4-5) (Factor A). The pampas cat is a known predator of Junin rails (BLI 2008, p. 4; BLI 2009b, p 2). The ground nesting Junin rail is particularly vulnerable to predation near the end of the dry season, when nesting begins and the vegetative cover of the marshlands within which they build their nests (BLI 2009b, p. 2) is more exposed and accessible to predators (ParksWatch 2006, p. 23). The Junin rail's small population size, combined with its restricted range and threat of predation, increases the species' vulnerability to adverse natural events and manmade activities that destroy individuals and their habitat (Holsinger 2000, pp. 64-65; Primack 1998, pp. 279-308; Young and Clarke 2000, pp. 361- 366). Summary of Factor E The Junin rail has a small population size that renders it vulnerable to genetic risks that negatively impact the species' viability. The species occupies a restricted range and occurs in habitat that continues to be altered and destroyed due to human activities (Factor A). Predation jeopardizes the species' already small population size because it results in the direct removal of Junin rail individuals from the population, can remove potentially reproductive adults from the breeding pool, and could lead to extirpation (Factor C). The small population size, as well as its restricted range and threat of predation, increases the species' vulnerability to extinction through demographic or environmental fluctuations. Based on the species' small population size, restricted range, and threat of predation, we have determined that the Junin rail is particularly vulnerable to the threat of adverse natural events (e.g., genetic, demographic, or environmental) and human activities (e.g., water level manipulation, contamination, cattail harvest, and overgrazing) that destroy individuals and their habitat. The genetic and demographic risks associated with small population sizes are exacerbated by ongoing human activities that continue to curtail the species' habitat throughout its range. The species' population has declined and is predicted to continue declining due to an ongoing reduction in the quality of its habitat (Factor A). Therefore, we find that the species' small population size, in concert with its restricted range, threat of predation, and its heightened vulnerability to adverse natural events and manmade activities, are threats to the continued existence of the Junin rail throughout its range. Status Determination for the Junin Rail The Junin rail is a ground nesting bird endemic to Lake Junin, found at 13,390 ft (4,080 m) above sea level in Peru, where it resides year-round. The species has high habitat specificity and occurs only in two localities within the marshland mosaic habitat that surrounds the lake. The current estimated range of the species is 62 mi\2\ (160 km\2\), and its population size is estimated to be 1,000-2,499. However, [[Page 628]] both of these figures are likely to be overestimates; despite suggestions that the species inhabits the entire area of marshland surrounding the lake, the species has only been confirmed in two localities. We have carefully assessed the best available scientific and commercial information regarding the past, present, and potential future threats faced by the Junin rail and have concluded that there are four primary factors that threaten the continued existence of the rail: (1) Habitat destruction, fragmentation, and degradation; (2) disease and predation; (3) limited size and isolation of remaining populations; and (4) inadequate regulatory mechanisms. Human activities that degrade, alter, and destroy habitat are ongoing throughout the Junin rail's range. Lake waters are artificially manipulated to produce hydropower, resulting in reductions in water levels that impact the species' nesting and foraging sites, especially near the end of the dry season when this ground nesting bird begins breeding. Reduced water levels and aquatic contaminants have permanently destroyed segments of cattail communities, compromising the amount of suitable flooded marshland available for nesting. Climate fluctuations exacerbate the effects of habitat alteration, as droughts further decrease available nesting grounds and excessive rains increase contaminant runoff into the lake (Factor A). Environmental contaminants have caused mortality in other waterbirds and have likely reduced the overall general health of the Junin rail population (Factor C). Overgrazing and cattail harvest and burning further alter and destroy the marshland upon which the rails depend (Factor A). Water drawdown exposes the birds and their marshland nesting areas to greater access by grazing animals, cattail harvesters, and predators (Factors A and C). Junin rail habitat continues to be altered by human activities, which results in the continued degradation and destruction of habitat and reduces the quality and distribution of remaining suitable habitat. Habitat loss was a factor in the Junin rail's historical decline (see Historical Range and Distribution), and the species is considered to be declining today due to continued reduction of available habitat (Factors A and E). The Junin rail population is small, increasing the species' vulnerability to the threat of adverse natural events (e.g., genetic, demographic, or environmental) and human activities (e.g., water contamination, water level manipulation, cattail harvest, and overgrazing) that destroy individuals and their habitat. Human activities that continue to curtail the species' habitat throughout its range exacerbate the genetic and demographic risks associated with small population sizes (Factor E). Predation jeopardizes the species' already small population size because it results in the direct removal of Junin rail individuals from the population, can remove potentially reproductive adults from the breeding pool, and could lead to extirpation (Factor C). The Junin rail population has declined at a rate between 10 and 19 percent during the past 10 years (see Population Estimates), and this decline is predicted to continue commensurate with ongoing threats from habitat destruction, water contamination, overgrazing, and cattail harvest and burning (Factor A). Despite the species' endangered status in Peru and its occurrence entirely within one protected area (Factor D), habitat destruction and degradation continue as a result of human activities that alter lake levels and compromise water quality and increase the species' susceptibility to overgrazing and predation (Factors A and C). Therefore, regulatory mechanisms are either inadequate or ineffective at mitigating the existing threats to the species and its habitat (Factor D). Section 3 of the Act defines an ``endangered species'' as ``any species which is in danger of extinction throughout all or a significant portion of its range'' and a ``threatened species'' as ``any species which is likely to become an endangered species within the foreseeable future throughout all or a significant portion of its range.'' Based on the immediate and ongoing threats to the Junin rail throughout its entire range, as described above, we determine that the Junin rail is in danger of extinction throughout all of its range. Therefore, on the basis of the best available scientific and commercial information, we are proposing to list the Junin rail as an endangered species throughout all of its range. IV. Peruvian plantcutter (Phytotoma raimondii) Species Description The Peruvian plantcutter, locally known as ``cortarrama Peruana,'' is a small finch-like bird endemic to the dry forests of coastal northwest Peru (Collar et al. 1992, p. 805; Goodall 1965, p. 636; Ridgely and Tudor 1994, p. 733; Sibley and Monroe 1990, p. 371; Schulenberg et al. 2007, p. 488; Walther 2004, p. 73). The Peruvian plantcutter is an herbivore with a predominantly leaf-eating diet (Bucher et al. 2003, p. 211; Schulenberg et al. 2007, p. 488; Walther 2004, p. 73). Plantcutters have bright yellow eyes, short wings and rather long tails, and their crown feathers form a slight crest (Goodall 1965, p. 635; Ridgely and Tudor 1994, p. 732). Adult birds are 7.28 to 7.48 in (18.5 to 9 cm) in length and weigh approximately 1.26 to 1.55 ounces (oz) (36 to 44 grams (g)) (Schulenberg et al. 2007, p. 488; Walther 2004, p. 73). Males are pale ashy gray, except a broad cinnamon-rufous color band on the belly and above the bill, and white colored bands on their wings (BLI 2000, p. 401; BLI 2009a, p. 1; Goodall 1965, p. 636; Ridgely and Tudor 1994, p. 733). Females are buff-brown with broad, dark brown stripes above, and white with heavy black-striped underparts (BLI 2009a, p. 1; Collar et al. 1992, p. 805). Juvenile birds have not been described (Walther 2004, p. 73). The Peruvian plantcutter's bill is stout, short, conical, and finely serrated with sharp tooth-like projections that run the length of the beak on both sides, and which are well suited for plucking buds, leaves, shoots, and fruits (Goodall 1965, p. 635; Ridgely and Tudor 1994, p. 732; Schulenberg et al. 2007, p. 488) (see Habitat and Life History). Taxonomy The Peruvian plantcutter was first taxonomically described as Phytotoma raimondii by Taczanowski in 1883 (ITIS 2009, p. 1; Sibley and Monroe 1990, p. 371). The type-specimen of the Peruvian plantcutter (the specimen that was described by Taczanowski) was collected by the ornithologist Konstanty Jelski, who recorded the specimen as being collected in the Tumbes Department of Peru (Flanagan et al. in litt. 2009, p. 2). However, the reported collection location may have been inaccurate (see Historical range and Distribution, below). The genus Phytotoma contains three species of plantcutters, all endemic to South America (Dickinson 2003, p. 346; Goodall 1965, p. 635; Sibley and Monroe 1990, p. 371; Walther 2004, p. 73). Ornithologists have long debated to which family this genus belongs. Some ornithologists have recommended that the genus be placed in its own family, Phytotomidae (Lanyon and Lanyon 1989, p. 422), while others placed the genus within the Tyrannidae family (Sibley and Monroe 1990, p. 371). Molecular research using DNA sequencing supports the inclusion of Phytotoma in the Cotingidae family (Irestedt et al. 2001, p. 23; Johansson et al. 2002, p. 993; Ohlson et al. 2006, p. 10). The Cotingidae family includes a [[Page 629]] wide diversity of species that are found in Central America and tropical South America (Snow 2004, p. 32). Therefore, based on the information currently available to us, we accept that the Peruvian plantcutter belongs to the Cotingidae family, which also follows the Integrated Taxonomic Information System (ITIS 2009, p. 1). Habitat and Life History The Peruvian plantcutter is reportedly selective in its habitat preference and requires a variety of arid tree and shrub species with dense low-hanging branches to the ground (Collar et al. 1992, p. 805; Flanagan and More 2003, p. 5; Flanagan et al. in litt. 2009, p. 7; Williams 2005, p. 2). The primary habitat for the Peruvian plantcutter is seasonally dry tropical forest, which is also referred to equatorial dry tropical forest, and which occurs in the semiarid lowlands of northwest Peru (Linares-Palomino 2006, pp. 260, 263-266; Schulenberg et al. 2007, p. 21; Walther 2004, p. 73). The Peruvian plantcutter also uses arid lowland scrub (dense and open) and dense riparian shrub communities (BLI 2009a. p. 2; Collar et al. 1992, p. 805; Schulenberg et al. 2007, pp. 21, 488; Stotz et al. 1996, p. 19; Walther 2004, p. 73). Stotz et al. (1996, pp. 19, 428) identified the Peruvian plantcutter as a key indicator species for Equatorial Pacific Coast arid lowland scrub. The lowland dry tropical forest and scrub are characterized as small and heavily fragmented patches of plant species adapted to the arid conditions of the prolonged dry season of northwest Peru (Best and Kessler 1995, p. 40; Bridgewater et al. 2003, pp. 132, 140; Ridgely and Tudor 1994, p. 734). The lowland dry forest or woodlands in northwest Peru are open canopied, with trees occurring in scattered clumps or individually (Flanagan and More 2003, p. 4). The dominant tree species of the lowland dry forest is Prosopis pallida (common name ``kiawe;'' also locally referred to as ``algarrobo'') in the Fabaceae family (legume family) (Lopez et al. 2005, p. 542; More 2002, p. 39). Prosopis pallida is a wide-spreading tree or large shrub, 26-65 ft (8-20 m) tall, with dense branches; spines can be present or absent (Pasiecznik et al. 2001, p. 36). This deep-rooted drought-tolerant species, related to mesquite species of southwest United States and Mexico, provides an important ecological function by improving and stabilizing soil conditions (Brewbaker 1987, p. 1; Pasiecznik et al. 2001, pp. 101-102). Typical of legumes, P. pallida is able to ``fix'' atmospheric nitrogen for plant utilization and growth (Brewbaker 1987, p. 1; Pasiecznik et al. 2001, p. 3). Three of the most common tree species associated with P. pallida dry forest habitat used by the Peruvian plantcutter are Capparis scabrida (no common name, but locally known as ``sapote''), in the Capparaceae (caper) family, and Acacia macracantha (long-spine acacia, locally known as ``faique'') and Parkinsonia aculeata (Jerusalem thorn, locally known as ``palo verde''), both in the Fabaceae (legume) family (More 2002, pp. 17-23). Associated flowering shrubs in dry forest habitat include Capparis avicennifolia (no common name, but locally known as ``bichayo'') and C. crotonoides (no common name, but locally known as ``guayabito de gentil''), both in the Capparaceae (caper) family; Cordia lutea (no common name, but locally known as ``overall'') in the Boraginaceae (borage) family; and Maytenus octogona (no common name, but locally known as ``realengo'') in the Celastraceae (bittersweet) family. Other commonly occurring dry forest vegetation includes vines (e.g., Convolvulaceae (morning-glory) and Cucurbitaceae (gourd) families), Psittacanthus chanduyensis (tropical mistletoe; locally known as ``suelda con suelda'') in the Loranthaceae (mistletoe) family, scattered herbaceous species (e.g., Asteraceae (sunflower), Scrophulariaceae (figwort), and Solanaceae (nightshade) families), and grasses (e.g., Poaceae (grass) family) (Elton 2004, p. 2; Ferreyera 1983, pp. 248-250; More 2002, pp. 14-17; Walther 2004, p. 73). Riparian vegetation includes dense shrub and small trees of P. pallida, A. macracantha, Capparis spp., and Salix spp. (willow spp.) (Lanyon 1975, p. 443). The arid climate of northwest Peru is due to the influence of the cold Humboldt Current that flows north, parallel to the Peruvian Coast (UNEP 2006, p. 16; Linares-Palomino 2006, p. 260; Rodriguez et al 2005, p. 2). The Humboldt Current has a cooling influence on the climate of coastal Peru, as the marine air is cooled by the cold current and, thus, is not conducive to generating rain. To the east, the Andean Mountains prevent humid air from the Amazon from reaching the western lowlands (Lanyon 1975, p. 443; Linares-Palomino 2006, p. 260). Coastal northwest Peru experiences a short rainy season during the summer months (January-April) (Linares-Palomino 2006, p. 260), which can also include precipitation in the form of mist or fine drizzle along the coast (Lanyon 1975, p. 443). The mean annual precipitation across the range of the Peruvian plantcutter is 0.196 to 3.80 in (5.0 to 99 mm) (hyper-arid to arid) (Galan de Mera et al. 1997, p. 351). The climate is warm and dry with the annual temperature range of 74[deg] to 77[deg] F (23[deg] to 25[deg] C) at elevations below 1,968 ft (600 m) (Linares-Palomino 2006, p. 260). Northwest Peru is strongly influenced by the El Ni[ntilde]o Southern Oscillation (ENSO) cycle (Rodriguez et al 2005, p. 1), which can have particularly profound and long-lasting effects on arid terrestrial ecosystems (Holmgren et al. 2006a, p. 87; Mooers et al. 2007, p. 2) (see Factor A). Knowledge of the breeding of most species within the Cotingidae family, including the Peruvian plantcutter, is not well known (Walther 2004, p. 73). The Peruvian plantcutter is considered a resident species in Peru, which indicates that it breeds there (Snow 2004, p. 61; Walther 2004, p. 73). Nesting activity of plantcutters appears to occur from March to April (Collar et al. 1992, p. 805; Walther 2004, p. 73). Plantcutters build shallow, cup-shaped nests that are made of thin dry twigs and lined with root fibers and other softer material (Snow 2004, p. 55). Nests range from 2.4 to 3.5 in (6 to 9 cm) in height and 3.9 to 7.0 in (10 to 18 cm) in diameter, and can be placed 3.3 to 9.8 ft (1 to 3 m) above the ground inside a thick thorny shrub or higher in the fork of a tree (Elton 2004, p. 2; Flanagan and More 2003, p. 3; Snow 2004, p. 55). Each female lays two to four eggs, and the incubation period lasts about 2 weeks (Snow 2004, p. 56; Goodall 1965, p. 636; Walther 2004, p. 73). The eggs have been described as short oval to sub- elliptical in shape and grayish olive in color with dark brownish olive spots at the larger end (Flanagan and Millen 2008, p. 1). Males assist rearing the chicks, which fledge after 17 days or so (Snow 2004, p. 56). Plantcutters are herbivores with a predominantly leaf-eating diet (Bucher et al. 2003, p. 211; Snow 2004, p. 46). As an herbivore, the Peruvian plantcutter is dependent on year-round availability of high- quality food, particularly during the dry season when plant growth is very limited (Bucher et al. 2003, p. 216). Peruvian plantcutters eat buds, leaves, and shoots of P. pallida and various other trees and shrubs, as well as some fruits (e.g., mistletoe) (Goodall 1965, p. 635; Schulenberg et al. 2007, p. 488; Walther 2004, p. 73). The seeds, green seed pods, leaves, and flowers of P. pallida provide a protein-rich food source for animals (Lewis et al. 2006, p. 282). Research studies on the two related plantcutter species, the rufous-tailed plantcutter (P. rara) and the white-tipped plantcutter (P. rutila), showed that the herbivore diet of these [[Page 630]] two species did not affect the energy levels of observed birds (Lopez- Calleja and Bozinovic 1999, p. 709; Meynard et al. 1999, p. 906; Rezende et al. 2001, p. 783). The Peruvian plantcutter appears to prefer to feed while perched in shrubs and trees, although individuals also have been observed foraging on the ground (Snow 2004, p. 50). Birds have been observed in pairs and small groups (Collar et al. 1992, p. 804; Flanagan and More 2003, p. 3; Schulenberg et al. 2007, p. 488; Walther 2004, p. 73). Historical Range and Distribution The Peruvian plantcutter is a restricted-range species that is confined to the mostly flat, narrow desert zone, which is less than 31 mi (50 km) in width (Lanyon 1975, p. 443) and runs along the coast of northwest Peru (Ridgely and Tudor 1994, p. 734; Stattersfield et al. 1998, p. 213; Walther 2004, p. 73). The historical range of the Peruvian plantcutter reportedly extended from the town of Tumbes, located in extreme northwest corner of Peru and approximately south to north of Lima within the Regions of Tumbes, Piura, Lambayeque, La Libertad, Ancash, and Lima (from north to south) (Collar et al. 1992, pp. 804-805). The historical distribution of the Peruvian plantcutter was most likely throughout the contiguous lowland P. pallida dry forest and riparian vegetation, below 1,804 ft (550 m) (Collar et al. 1992; Williams 2005, p. 1). According to Collar et al. (1992, pp. 804-805), the Peruvian plantcutter is known from 14 historical sites. The type-specimen of the Peruvian plantcutter, which was collected and labeled by Konstanty Jelski as being found in Tumbes in the late 1870s, was most likely collected south of the town of Tumbes (Flanagan et al. in litt. 2009, pp. 2, 15). It is unknown whether the type specimen was lost or destroyed, or if it was ever returned to Peru (Flanagan et al. in litt. 2009, p. 2). Today, there is good indication that the type-specimen was mislabeled as being collected in Tumbes (Flanagan et al. in litt. 2009, pp. 2). Although the Tumbes Region has been extensively surveyed for the Peruvian plantcutter, including the North-West Biosphere Reserve, there have never been other collections in or near the vicinity of Tumbes or other evidence to suggest that the Peruvian plantcutter ever occurred in the area (Flanagan et al. in litt. 2009, p. 2). Thus, it appears that the Peruvian plantcutter never occurred in the Tumbes Region. Researchers consider the reduction in dry forest habitat to be the result of historical human activities, including extensive land clearing for agriculture, timber and firewood extraction, charcoal production, and overgrazing. These activities have led to the reduction and severe fragmentation of dry forest habitat today (BLI 2009a, pp. 2- 3; Bridgewater et al. 2003, p. 132; Flanagan et al. in litt. 2009, pp. 1-9; Lanyon 1975, p. 443; Lopez et al. 2006, p. 898; Pasiecznik et al. 2001, pp. 10, 75, 78, 95; Ridgely and Tudor 1994. p. 734; Schulenberg et al. 2007, p. 488; Stotz et al. 1998, p. 52) (see Factor A). Current Range and Distribution The current range of the Peruvian plantcutter is approximately 1,892 mi\2\ (4,900 km\2\) (BLI 2009a, p. 1), which is between 33 and 1, 804 ft (10 and 550 m) above sea level and within the Peruvian Regions of Piura, Lambayeque, Cajamarca, La Libertad, and Ancash (from north to south) (Flanagan et al. in litt. 2009, pp. 14-15). The species' reported range is an overestimate because BirdLife International defines a species' range as the total area within its extent of occurrence (see Current Range and Distribution of the ash-breasted tit- tyrant) (BLI 2000, pp. 22, 27). The Peruvian plantcutter's current distribution is severely fragmented and distributed amongst small, widely separated remnant patches of P. pallida dry forest (BLI 2009a, pp. 2-3; Flanagan et al. in litt. 2009, pp. 1-9; Ridgely and Tudor 1994. p. 18), which are usually heavily disturbed fragments of forest (Bridgewater et al. 2003, p. 132). Therefore, the species' actual range is smaller than this figure. The Peruvian plantcutter is extirpated from 11 of its 14 historical sites due to loss of habitat or degradation of habitat (Elton 2004, p. 1; Flanagan and More 2003, p. 5; Hinze 2004, p. 1). Depending on habitat quality, it is estimated that the Peruvian plantcutter requires approximately 2.5 ac (1 ha) of habitat for suitable food and nesting sites (Flanagan and More 2003, p. 3; Flanagan et al. in litt. 2009, p. 7). Although the Peruvian plantcutter has been found in patches of P. pallida dry forest habitat that are in close proximity to agricultural lands, tracks or roads, and human settlement (Flanagan et al. in litt. 2009, pp. 2-7), much of the available P. pallida dry forest habitat is unoccupied (BLI 2000, p. 401; Schulenberg et al. 2007, p. 488; Snow 2004, p. 69; Walther 2004, p. 73). Flanagan et al. (in litt. 2009, pp. 1-15) recently completed a comprehensive review of 53 locations where there have been documented sightings of the Peruvian plantcutter, of which the authors determined 29 sites were extant. Flanagan et al. (in litt. 2009, pp. 2-4, 14) reported that 17 of the 22 documented sites of the Peruvian plantcutter in the Piura Region are extant. In this region, Talara Province contains the largest concentration of intact P. pallida dry forest habitat in northwest Peru and the largest subpopulation of the Peruvian plantcutter (BLI 2009a, p. 2; Flanagan et al. in litt. 2009, p. 3; Flanagan and More 2003, p. 5; Walther 2004, p. 73). Additionally, there are several other documented sites of the Peruvian plantcutter in the Piura Region (e.g., Manglares de San Pedro, Illescas Peninsula, and Cerro Illescas) (BLI 2009c, p. 1; Flanagan et. al. in litt. 2009, pp. 4, 14). Flanagan et al. (in litt. 2009, pp. 4-5, 14) reported a total of 13 locations of the Peruvian plantcutter in the Lambayeque Region, of which 5 are considered extant. Within the Region, there are four important areas for the Peruvian plantcutter: (1) The Pomac Forest Historical Sanctuary (Santuario Historico de Bosque de Pomac), designated as a protected archeological site in 2001, is comprised of 14,547 ac (5,887 ha) of P. pallida dry forest (BLI 2009e, p. 1; Flanagan et al. in litt. 2009, p. 4). The Sanctuary includes the archeological site Batan Grande, an area comprised of 1,235 ac (500 ha) of P. pallida dry forest (BLI 2009e, p. 1; Flanagan et al. in litt. 2009, p. 4). (2) Near the small town of Rafan are remnant patches of P. pallida dry forest, encompassing approximately 3,706 ac (1,500 ha) (BLI 2009f, p. 1). The Rafan area has become a popular birding site for the Peruvian plantcutter (BLI 2009f, p. 1; Engblom 1998, p. 1). (3) Murales Forest (Bosque de Murales), comprised of P. pallida dry forest, is a designated Archeological Reserved Zone (BLI 2009a, p. 3; Stattersfield et al. 2000, p. 402). (4) Chaparri Ecological Reserve, comprised of 85,033 ac (34,412 ha) with P. pallida dry forest, is a community-owned and managed protected area (Walther 2004, p. 73). The remaining sites in the Lambayeque Region are small remnant patches of P. pallida dry forest and comprised of a few acres (Flanagan et al. in litt. 2009, pp. 4-5; Walther 2004, p. 73). The protected areas are further discussed under Factors A and D. Flanagan et al. (in litt. 2009, pp. 5, 14) reported one occupied site of the Peruvian plantcutter in the Cajamarca Region, consisting of approximately 14.8 ac (6 ha) of remnant P. pallida dry forest in the Rio Chicama Valley. Six of the 12 known sites of the Peruvian plantcutter in the La Libertad Region are considered extant (Flanagan et al. in litt. 2009, pp. 5-6, 14). Each of these sites consists of small patches of remnant P. [[Page 631]] pallida dry forest habitat (Walther 2004, p. 73; Flanagan et al. in litt. 2009, pp. 5-6). Of the three known sites of the Peruvian plantcutter in the Ancash Region, only one is reported to be extant (Flanagan et al. in litt. 2009, pp. 6, 14). Additionally, the authors reported that the two historical sites in the Lima Region were also unoccupied in the most recent survey (Flanagan et al. in litt. 2009, pp. 7, 15). In summary, the extant population of the Peruvian plantcutter is comprised of two disjunct subpopulations (BLI 2009g, pp. 1-2; Walther 2004, p. 73), with several smaller sites (Flanagan and More 2003, pp. 5-9; Flanagan et al. in litt. 2009, pp. 2-7; Walther 2004, p. 73; Williams 2005, p. 1). Additional surveys are needed to determine if available P. pallida dry forest habitat is occupied by the Peruvian plantcutter (Flanagan et al. in litt. 2009, p. 7). Population Estimates There have been no rigorous quantitative assessments of the Peruvian plantcutter's population size (Williams 2005, p. 1). The estimated extant population size is between 500 and 1,000 individuals, and is comprised of 2 disjunct subpopulations (BLI 2009g, pp. 1-2; Walther 2004, p. 73) and several smaller sites (Flanagan and More 2003, pp. 5-9; Flanagan et al. in litt. 2009, pp. 2-7; Walther 2004, p. 73; Williams 2005, p. 1). The northern subpopulation, located in the Talara Province in Piura Region, reportedly has between 400 and 600 individuals, or approximately 60 to 80 percent of the total population of the Peruvian plantcutter (BLI 2009a, p. 2; Snow 2004, p. 69; Walther 2004, p. 73; Williams 2005, p. 1). The second subpopulation, located at Pomac Forest Historical Sanctuary (Lambayeque Region), reportedly has 20 to 60 individuals (BLI 2009a, p. 2; BLI 2009e 2009, p. 1; Walther 2004, p. 73). The smaller sites are estimated to consist of a few individuals, up to 40 individuals (Flanagan and More 2003, pp. 5-9; Flanagan et al. in litt. 2009, pp. 2-7; Walther 2004, p. 73; Williams 2005, p. 1). The population estimate for the Peruvian plantcutter--that is, the total number of mature individuals--is not the same as the effective population size (i.e., the number of individuals that actually contribute to the next generation). Further, the subpopulation structure and the extent of interbreeding among the occurrences of the Peruvian plantcutter are unknown. Although the two large subpopulations and many of the smaller occurrences of the Peruvian plantcutter are widely separated (BLI 2009a, pp. 2-3; Flanagan et al. in litt. 2009, pp. 1-9; Ridgely and Tudor 1994. p. 18), there is insufficient information to determine whether these occurrences function as genetically isolated subpopulations. The Peruvian plantcutter has experienced a population decline of between 1 and 9 percent in the past 10 years, and this rate of decline is predicted to continue (BLI 2009g, p. 1). The population is considered to be declining in close association with continued habitat loss and degradation of habitat (see Factors A and E) (BLI 2009a, pp. 1-3; BLI 2009g, pp. 1-3; Ridgely and Tudor 1994, p. 18; Snow 2004, p. 69). Conservation Status The Peruvian plantcutter is considered ``endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276855). The IUCN considers the Peruvian plantcutter to be ``Endangered'' because of ongoing habitat destruction and degradation of its small and severely fragmented range (BLI 2000, p. 402; BLI 2009a, pp. 2-3; BLI 2009g 2009, pp. 1-2). From 1996 to 2000, the IUCN considered the Peruvian plantcutter to be ``Critically Endangered'' (BLI 2009g, p. 1), following changes to the IUCN listing criteria in 2001. Experts have suggested returning the the species to its previous classification of ``Critically Endangered,'' due to the numerous and immediate threats to the species (Jeremy N. M. Flanagan, Conservation Biologist, Sullana, Peru, in litt. 2009 e-mail to DSA, p. 1; Snow 2004, p. 69; Walther 2004, p. 74). The Peruvian plantcutter occurs within two protected areas in Peru. It has been documented in the P. pallida dry forest within the protected archeological sites of the Pomac Forest Historical Sanctuary (BLI 2009e, p. 1) and Murales Forest (Walther 2004, p. 73). Summary of Factors Affecting the Peruvian plantcutter A. The Present or Threatened Destruction, Modification, or Curtailment of the Species' Habitat or Range The Peruvian plantcutter is dependent upon undisturbed Prosopis pallida dry forest with good floristic diversity (Collar et al. 1992, p. 805; Engblom 1998, p. 1; Flanagan and More 2003, p. 4). In northwest Peru, P. pallida dry forest was historically contiguous, covering approximately 2,703 m\2\ (7,000 km\2\) of the coastal lowland of northwest Peru (Ferreyera 1983, p. 248). There were also extensive wooded stands of small to medium trees of P. pallida, Acacia spp., Capparis spp., and willows (Salix spp.) along permanent lowland rivers, which have since been cleared for agricultural purposes (Lanyon 1975, p. 443). Today, with the exception of three relatively large intact dry forests (i.e., Talara Province, Murales Forest, and Pomac Forest Historical Sanctuary), the vast majority of P. pallida dry forest, arid lowland scrub, and riparian vegetation has been reduced due to human activities. Seasonally dry tropical forests are considered the most threatened of all major tropical forest types (Janzen 1988, p. 130), with higher threat levels than any other Neotropical habitat (Stotz et al. 1996, p. 51). The Peruvian plantcutter has been extirpated from most of its historical sites due to loss or degradation of habitat (Elton 2004, p. 1; Flanagan et. al. in litt. 2009, pp. 1-15; Flanagan and More 2003, pp. 5-9; Snow 2004, p. 69). Current information indicates that the vast majority of occupied sites of the Peruvian plantcutter are small, remnant, disjunct patches of P. pallida dry forest with each a few acres in size (BLI 2000, p. 402; Flanagan et. al. in litt. 2009, pp. 2-7; Snow 2004, p. 69; Walther 2004, p. 73). Habitat loss, conversion, and degradation throughout the Peruvian plantcutter's range have been and continue to occur as a result of human activities (BLI 2000, p. 402; BLI 2009a, p. 2; BLI 2009b, p. 1; BLI 2009e, p. 1; BLI 2009f, p. 1; Bridgewater et al. 2003, p. 132; Flanagan et al. in litt. 2009, p. 15; Lanyon 1975, p. 443; Schulenberg et al. 2007, p. 488; Snow 2004, p. 69; Walther 2004, p. 73), including: (1) Clearcutting and burning of dry forest for agriculture and other purposes (BLI 2000, p. 402; BLI 2009a, p. 2; BLI 2009d, pp. 1-2; BLI 2009f, p. 1; BLI 2009g, p. 2; Bridgewater et al. 2003, p. 132; Collar et al. 1992, p. 806; Engblom 1998, p. 1; Flanagan et al. 2005, p. 244; Ridgely and Tudor 1994, p. 734; Snow 2004, p. 69; Walther 2004, p. 73; Williams 2005, p. 2); (2) Extraction activities, including cutting for timber, firewood, and charcoal production (Best and Kessler 1995, p. 196; BLI 2000, p. 402; BLI 2009d, pp. 1-2; BLI 2009g, p. 2; Ridgely and Tudor 1994, p. 734; Rodriguez et al. 2007, p. 269; Snow 2004, p. 69; Williams 2005, p. 1); (3) Grazing by goats of P. pallida dry forests, and arid scrub and riparian vegetation (Capra species) (BLI 2000, p. 402; BLI 2009a, p. 2; BLI 2009b, p. 1; BLI 2009d, pp. 1-2; Best and Kessler [[Page 632]] 1995, p. 196; More 2002, p. 37; Snow 2004, p. 69); (4) Human encroachment (Fernandez-Baca et al. 2007, p. 45); and (5) Unpredictable climate fluctuations that exacerbate human activities and encourage further habitat destruction (Block and Richter 2007, p. 1; Jetz et al. 2007, p. 1211; Richter 2005, p. 26). The vast majority of P. pallida dry forest habitat has been converted to commercial agricultural production, which is the primary factor in the historical decline of the Peruvian plantcutter (BLI 2000, p. 402; BLI 2009a, p. 2; BLI 2009d, pp. 1-2; BLI 2009f, p. 1; Collar et al. 1992, p. 806; Engblom 1998, p. 1; Ridgely and Tudor 1994, p. 734; Snow 2004, p. 69; Walther 2004, p. 73; Williams 2005, p. 2). Agriculture in the coastal lowlands of northwest Peru consists of modern large, privately owned farms and large cooperatives that primarily produce crops (e.g., sugarcane, cotton, rice) for export (Lanyon 1975, p. 443; Roethke 2003, pp. 58-59). Continual habitat destruction and degradation of the dry forest is mostly due to firewood cutting and charcoal production. Prosopis pallida is the dominant tree of the dry forest habitat, and is highly sought after because the wood provides an important source of high- quality cooking fuel (Brewbaker 1987, p. 1; Pasiecznik et al. 2001, p. 75). Throughout the Peruvian plantcutter's range, whole trees, branches, and roots of P. pallida are cut for firewood and production of charcoal, which is used for cooking fuel in homes, restaurants, and businesses that use brick kilns, both locally and in urban centers (Flanagan et al. in litt. 2009, p. 7). Wood of P. pallida is also used for construction and fence posts (Pasiecznik et al. 2001, p. 78). Additionally, roots of older P. pallida trees are used in wooden art crafts (BLI 2009a, p. 2). Talara Province (in Piura Region) contains the largest remaining intact P. pallida dry forest in northwest Peru, encompassing approximately 123,553 ac (50,000 ha) (Flanagan et al. in litt. 2009, pp. 2-3; Flanagan and More 2003, p. 5; Walther 2004, p. 73). The Province also has the largest subpopulation of the Peruvian plantcutter, reportedly between 400 and 600 individuals or approximately 60 to 80 percent of the total population (BLI 2009a, p. 2; Elton 2004, pp. 3-4; Snow 2004, p. 69; Walther 2004, p. 73; Williams 2005, p. 1). Until recently, a large portion of the Province, including P. pallida dry forest habitat, was owned by the State-owned petroleum company PetroPeru, which prohibited access to approximately 90,000 ac (36,422 ha). Under the management of PetroPeru, the P. pallida dry forest was not subject to the same habitat destruction and degradation activities (e.g., clearing of trees, firewood cutting and charcoal production) as other dry forest habitat areas (Elton 2004, pp. 3-4; Hinze 2004, p. 1). Recently, the land was reverted to the Peruvian Government, and it is unclear whether the government plans to issue private concessions as in other areas of the Province (Elton 2004, p. 4). Consequently there have been recent efforts, including a formal petition to the Peruvian Government, to create a 12,000 to 24,710-ac (4,856- to 10,000-ha) protected reserve for the northern subpopulation of the Peruvian plantcutter (Elton 2004, p. 4; Walther 2004, p. 73). However, the government has not designated such a reserve for the species (Elton 2004, p. 4; Williams 2005, p. 3). Habitat destruction and degradation of P. pallida dry forest, including firewood cutting and charcoal production, is ongoing in the Talara Province, including on the land previously owned by PetroPeru and an area identified as the Talara Important Birding Area by BirdLife International (Flanagan in litt. 2009, p. 1). Since 2005, there has been extensive cutting and clearing of P. pallida trees for fuel to cook and dry Humboldt giant squid (Dosidicus gigas) carcasses (Flanagan et al. in litt. 2009, p. 8). The most important commercial fishery of the Humboldt giant squid occurs along the coast of Peru (UNEP 2006, p. 33; Zeidberg and Robison, 2007, p. 12, 948). Harvested carcasses are transported by truck from the Talara port to recently cleared areas in the dry forest, where they are boiled and dried (Flanagan et al. in litt. 2009, p. 8). Therefore, this fishery not only adds to the collection pressure on Prosopis species for use as fuel, but also adds to forest clearing in the area. Another relatively new demand for P. pallida firewood is associated with the illegal extraction of crude oil from above-ground pipes in the Talara Province. The stolen oil is distilled by heating it with firewood (Flanagan et al. in litt. 2009, p. 8). Capparis scabrida (no common name, but locally known as ``sapote'') is a tree that occurs with P. pallida and is also a food source for the Peruvian plantcutter. Although the tree is listed as ``Critically Endangered'' by the Peruvian Government, the highly sought after wood is cut to produce handicrafts for the local, national, and international market, as well as for firewood and charcoal production (Rodriguez et al. 2007, p. 269). Habitat alteration is also caused by grazing goats, which remove or heavily degrade the shrubs and trees (BLI 2000, p. 402; BLI 2009a, p. 2; Elton 2004, pp. 3-4; Snow 2004, p. 69; Williams 2005, p 2). The seed pods and leaves of P. pallida provide highly nutritious fodder for goats (Brewbaker 1987, pp. 1-2; Pasiecznik et al. 2001, p. 95). Goats roam freely and graze on trees and shrubs, particularly lower branches close to ground which are preferred by the Peruvian plantcutter for foraging and nesting (Elton 2004, pp. 3-4; Snow 2004, p. 50; Williams 2005, p 2). Human encroachment and concomitant increasing human population pressures exacerbate the destructive effects of ongoing human activities (e.g., clearing of P. pallida dry forest, firewood cutting, and charcoal production) throughout the Peruvian plantcutter's range. Although the coastal lowlands represent only about 10 percent of country's total territory, more urban centers are located on the coast, which represent approximately 52 percent of the total population of Peru (Fernandez-Baca et al. 2007, p. 45). Larger concentrations of people put greater demand on the natural resources in the area, which spurs additional habitat destruction and increases infrastructure development that further facilitates encroachment. Peruvian plantcutters are also impacted by unpredictable climate fluctuations that exacerbate the effects of habitat fragmentation. Unpredictable climate fluctuations are more fully described under the Factor A analysis of the ash-breasted tit-tyrant and are summarized here. Changes in weather patterns, such as ENSO cycles (El Ni[ntilde]o and La Ni[ntilde]a events), tend to increase precipitation in normally dry areas, and decrease precipitation in normally wet areas (Holmgren et al. 2001, p. 89; TAO Project n.d., p. 1) while intensifying the effects of habitat fragmentation on the decline of a species (England 2000, p. 86; Holmgren et al. 2001, p. 89; Jetz et al. 2007, pp. 1211, 1213; Mora et al. 2007, p. 1027; Parmesan and Mathews 2005, p. 334; Plumart 2007, pp. 1-2; Timmermann 1999, p. 694), especially for narrow endemics (Jetz et al. 2007, p. 1213) such as the Peruvian plantcutter. The arid terrestrial ecosystem of Northwest Peru, where the Peruvian plantcutter occurs, is strongly influenced by the ENSO cycle (Rodriguez et al 2005, p. 1), which can have profound and long-lasting effects (Holmgren et al. 2006a, p. 87; Mooers et al. 2007, p. 2). The amount of rainfall during an El Ni[ntilde]o year can be more than 25 times greater than during normal years in northern Peru (Holmgren et al. [[Page 633]] 2006a, p. 90; Rodriguez et al 2005, p. 2). El Ni[ntilde]o events are important triggers for regeneration of plants in semiarid ecosystems, particularly the dry forest of northwest Peru (Holmgren et al. 2006a, p. 88; Lopez et al. 2006, p. 903; Rodriguez et al. 2005, pp. 2-3). During El Ni[ntilde]o events, plant communities and barren lands are transformed into lush vegetation, as seeds germinate and grow more quickly in response to increased rainfall (Holmgren et al. 2006a, p. 88; Holmgren et al. 2006b, pp. 2-8; Rodriguez et al. 2005, pp. 1-6). Over the last 20 years, recruitment of P. pallida in northwest Peru doubled during El Ni[ntilde]o years, when compared to non-El Ni[ntilde]o years (Holmgren et al. 2006b, p. 7). However, the abundant supply of vegetation encourages locals to expand goat breeding operations, which results in overgrazing by goats and further land degradation (Richter 2005, p. 26). ENSO cycles increase the risk of fire because El Ni[ntilde]o events are often followed by years of extremely dry weather (Block and Richter 2007, p. 1), and accumulated biomass dries and adds to the fuel load in the dry season (Block and Richter 2007, p. 1; Power et al. 2007, p. 898). Evidence suggests that the fire cycle in Peru has shortened, particularly coastal Peru and west of the Andes (Power et al. 2007, pp. 897-898), which can have broad ecological consequences (Block and Richter 2007, p. 1; Power et al. 2007, p. 898). According to Block and Richter (2007, p. 1), P. pallida dry forest and Capparis spp. scrublands in northwest Peru would likely experience a long-term change in plant species composition that favor aggressive, annual, non-native weedy plant species (Richter 2005, p. 26). An accelerated fire cycle would further exacerbate changes in species composition that hinder long-lived perennial, native plant species, such as Prosopis species, upon which the Peruvian plantcutter relies. ENSO cycles are ongoing, having occurred several times within the last decade (NWS 2009, p. 2), and evidence suggests that ENSO cycles have already increased in periodicity and severity (Richter 2005, pp. 24-25; Timmermann 1999, p. 694), which will exacerbate the negative impacts of habitat destruction on a species. It is predicted that, by 2050, approximately 11 to 16 percent of existing land is likely to be unsuitable for this species due to climate change; and, by 2100, it is predicted that about 24 to 35 percent of the species' range is likely to be lost as a direct result of global climate change (Jetz et al. 2007, p. 81). Habitat destruction is often caused by a combination of human activities that promote habitat degradation. In Lambayeque Region, a 3,706-ac (1,500-ha) section of remnant P. pallida dry forest is under continual threat from human activities, including conversion to agriculture, firewood cutting and charcoal production, and grazing by goats. This area may support between 20 and 40 Peruvian plantcutters (BLI 2009f, p. 1; Walther 2004, p. 73). In the 1990s, a significant portion of this dry forest was converted to sugarcane fields (Engblom in litt. 1998, p. 1; Snow 2004, p. 69; Walther 2004, p. 73; Williams 2005, p. 2). Within Piura and Lambayeque Regions, threats to the dry forest habitat include conversion to agriculture, firewood and timber cutting, and grazing by goats (BLI 2009d, pp. 1-2). Habitat destruction and alteration also occurs within two protected areas where the Peruvian plantcutter occurs (in Lambayeque Region), Pomac Forest Historical Sanctuary (Andean Air Mail and Peruvian Times 2009, p. 1; Flanagan et al. in litt. 2009, pp. 7-8; Williams 2005, p. 1), and the Murales Forest (BLI 2000, p. 402; BLI 2009a, p. 3; Stattersfield et al. 2000, p. 402; Walther 2004, p. 73). Habitat destruction and alteration activities within these protected areas are discussed under Factor D. Experts consider the population of this range-restricted endemic species to be declining in close association with the continued habitat loss and degradation (BLI 2000, p. 401; BLI 2009a, pp. 1-2; BLI 2009g, pp. 1-3), and that the effects are higher in dry forest habitat than in any other Neotropical habitat (Stotz et al. 1998, p. 51). Summary of Factor A The Peruvian plantcutter is dependent upon intact P. pallida dry forest with low-hanging branches and high floristic diversity, and associated arid lowland scrub and riparian vegetation. Prosopis pallida dry forest habitat, as well as arid lowland scrub and riparian shrub habitats, throughout Peruvian plantcutter's range have been and continue to be altered and destroyed as a result of human activities, including conversion to agriculture; timber and firewood cutting and charcoal production; grazing of goats; and human encroachment. Extant P. pallida dry forest today consists of remnant, disjunct patches of woodlands, which are heavily disturbed and under continued threat of degradation by human activities. Although observations suggest that this dry forest-dependent species is able to occupy very small remnant patches of dry forest with low-hanging branches and floristic diversity, and is able to persist to some degree near developed lands, many of these sites are approaching the lower threshold of the species' ecological requirements. This species has been extirpated from most of its historical sites due to loss or degradation of habitat. Additionally, many of the extant occupied sites are separated by great distances, which may lead to genetic isolation of the species (See Factor E). The same activities that caused the historical decline in this species are ongoing today. These habitat-altering activities are compounded by unexpected climate fluctuations, especially for narrow endemics, such as the Peruvian plantcutter. Excessive rains that are accompanied by El Ni[ntilde]o events induce further habitat destruction, as people take advantage of better grazing and growing climate conditions. Climate models predict that this species' habitat will continue to decline. Destruction of the remaining P. pallida dry forest fragments in Peru continues to reduce the quantity, quality, distribution, and regeneration of remaining patches of dry forest. Human activities that degrade, alter, and destroy habitat are ongoing throughout the species' range, including within the one protected area (Factor D). Therefore, we find that destruction and modification of habitat are threats to the continued existence of Peruvian plantcutter throughout its range. B. Overutilization for Commercial, Recreational, Scientific, or Educational Purposes We are not aware of any information currently available that indicates that overutilization of Peruvian plantcutter for commercial, recreation, scientific, or education purposes has occurred or is occurring at this time. As a result, we are not considering overutilization to be a threat to the continued existence of the Peruvian plantcutter. C. Disease or Predation We are not aware of any scientific or commercial information that indicates disease or predation poses a threat to the Peruvian plantcutter. As a result, we do not consider disease or predation to be a threat to the continued existence of the Peruvian plantcutter. D. The Inadequacy of Existing Regulatory Mechanisms The Peruvian plantcutter is considered ``endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276854). This Decree prohibits hunting, take, transport, and trade of protected species, except as permitted by regulation. As hunting, taking, or trade [[Page 634]] do not currently threaten the Peruvian plantcutter (Factor B), this regulation does not mitigate any current threats to the species. Peru has several categories of national habitat protection, which were described above as part of Factor D for the ash-breasted tit- tyrant (BLI 2008, p. 1; IUCN 1994, p. 2; Rodriguez and Young 2000, p. 330). The Peruvian plantcutter is known to occur within two Peruvian nationally protected areas, the Pomac Forest Historical Sanctuary and the Murales Forest (both Lambayeque Region). The Pomac Forest Historical Sanctuary supports an estimated 20 to 60 Peruvian plantcutters (BLI 2009a, p. 2; BLI 2009e, p. 1; Walther 2004, p. 73). Resources within the Pomac Forest Historical Sanctuary are managed for the preservation of the archeological site, P. pallida dry forest, and wildlife species. However, habitat destruction and alteration, including illegal forest clearing for farming, timber and firewood cutting, and grazing, continually threaten the Sanctuary (Williams 2005, p. 1). For 8 years, more than 250 families illegally occupied and farmed land in the Sanctuary. During the illegal occupancy, the inhabitants logged 4,942 ac (2,000 ha) of P. pallida trees for firewood and burned many other trees for charcoal production (Andean Air Mail and Peruvian Times 2009, p. 1). The logged forest was subsequently converted to agricultural crops, while remaining forest habitat was continually degraded by firewood cutting, charcoal production, and grazing of goats (Flanagan et al. in litt. 2009, p. 8.). In January 2009, the government forcibly removed the inhabitants, but it is too soon to determine the effect that habitat destruction has had on the suitability of the habitat for the Peruvian plantcutter. There is insufficient information to conclude that recent efforts to stop the illegal human occupancy of the area will have a positive impact on the species or remaining habitat within the protected area. Therefore, any protections afforded by this Sanctuary has not mitigated the threats to the species from ongoing habitat loss and associated population decline (Factor A). The Murales Forest is a designated Archeological Reserved Zone (BLI 2000, p. 401; BLI 2009a, p. 3; Stattersfield et al. 2000, p. 402), and supports a declining population of Peruvian plantcutters. According to Peruvian law, designation as a Reserved Zone allows for temporary protection while further study is under way to determine the area's importance (BLI 2008, p. 1; Rodriguez and Young 2000, p. 330). Although strict monitoring has protected some habitat (BLI 2009a, p. 3), the actual dry forest is not protected. In 1999, land rights to sections of the forest were sold for agricultural conversion, and government intervention has been necessary to prevent further sales of land for conversion to agriculture (BLI 2009a, p. 3). In 1999, Murales Forest and adjacent areas contained approximately 1,221 ac (494 ha) of habitat, and reportedly supported 140 Peruvian plantcutters (BLI 2000, p. 402). In 2004, the population was estimated to be 20 to 40 individuals (Walther 2004, p. 73). Therefore, the presence of the Peruvian plantcutter within this protected area has not mitigated the threats to the species from ongoing habitat loss and associated population decline (Factor A). Incidences of illegal activity that occur throughout the species' range also impact the Peruvian plantcutter. Ongoing firewood cutting and charcoal production degrades the small amount of remaining dry forest habitat throughout the species' range (BLI 2009d, pp. 1-2; BLI 2009g, p. 2; Ridgely and Tudor 1994, p. 734; Rodriguez et al. 2007, p. 269; Snow 2004, p. 69; Williams 2005, p. 1). In Talara Province (Piura Region), a recent increase in the illegal extraction of crude oil has generated further demand for P. pallida firewood, which is used as fuel to heat-distill the oil. According to Flanagan et al. (in litt. 2009, p. 8), enforcement to combat this illegal activity is difficult. Therefore, existing laws are ineffective at mitigating the ongoing threat of habitat destruction (Factor A). Summary of Factor D Peru has enacted various laws and regulatory mechanisms to protect and manage wildlife and their habitats. The Peruvian plantcutter is ``endangered'' under Peruvian law and occurs within two protected areas in Peru. As discussed under Factor A, the Peruvian plantcutter prefers P. pallida dry forest. This habitat has been drastically reduced and remaining habitat is comprised of small remnant patches of dry forest that are separated by great distances. Habitat throughout the species' range has been and continues to be destroyed and altered as a result of human activities, primarily conversion to agriculture, and continual degradation by timber and firewood harvest and charcoal production, and grazing by goats. These activities are ongoing, including within protected areas and despite the species' ``endangered'' status, indicating that the laws governing wildlife and habitat protection in Peru are either inadequate or inadequately enforced to protect the species or to mitigate ongoing habitat loss (Factor A) and population declines (Factor E). Therefore, we find that the existing regulatory mechanisms are inadequate to mitigate the current threats to the continued existence of the Peruvian plantcutter throughout its range. E. Other Natural or Manmade Factors Affecting the Continued Existence of the Species An additional factor that affects the continued existence of the Peruvian plantcutter is the species' small population size. BirdLife International has placed the Peruvian plantcutter in the population category of between 500 and 1,000 individuals (BLI 2009g, p. 1). The species' population size is not characterized as ``small'' in published literature and there is insufficient information on similar species (i.e., the other South American plantcutters) to understand whether the Peruvian plancutter's population size is small relative to other plantcutters. However, there are several indications that this number of individuals represents a small population. First, the Peruvian plantcutter's population size--which is defined by BirdLife International as the total number of mature individuals--is not the same as the effective population size--the number of individuals that actually contribute to the next generation (Shaffer 1981, pp. 132-133; Soule 1980, pp. 160-162). Not all individuals in a population will contribute to reproduction each year. Therefore, the estimated population size for the Peruvian plantcutter is an overestimate of the species' effective population size. Moreover, the subpopulation structure and the extent of interbreeding among the occurrences of the Peruvian plantcutter are unknown (see Population Estimates). If further research indicates that species does not breed as a single population, its effective population size would be further reduced. Second, the extant Peruvian plantcutter population occurs primarily in 2 disjunct subpopulations--Talara and Pomac Forest Historical Sanctuary (BLI 2009g, pp. 1-2; Walther 2004, p. 73)--and in several smaller sites (Flanagan and More 2003, pp. 5-9; Flanagan et al. in litt. 2009, pp. 2-7; Walther 2004, p. 73; Williams 2005, p. 1). Talara and Pomac Forest Historical Sanctuary are approximately 160 mi (257 km) apart (FCC (Federal Communications Commission)-Audio Division 2009). The Peruvian plantcutter is dependent upon [[Page 635]] undisturbed Prosopis pallida dry forest with good floristic diversity (Collar et al. 1992, p. 805; Engblom 1998, p. 1; Flanagan and More 2003, p. 4). Its habitat is heavily degraded and localities are small, severely fragmented, and widely separated (BLI 2009a, pp. 2-3; Bridgewater et al. 2003, p. 132; Flanagan et al. in litt. 2009, pp. 1- 9; Ridgely and Tudor 1994. p. 18) (see Factor A). It is possible that the distance between patches of suitable habitat is too great to support interbreeding between localities, so that the extant occurrences of this species would function as genetically isolated subpopulations. For these reasons, we consider the Peruvian plantcutter's current estimated population to be small and, as such, this species is subject to the risks associated with small population sizes. Small population size renders a species vulnerable to any of several risks, including inbreeding depression, loss of genetic variation, and accumulation of new mutations. Inbreeding can have individual or population-level consequences either by increasing the phenotypic expression (the outward appearance or observable structure, function, or behavior of a living organism) of recessive, deleterious alleles or by reducing the overall fitness of individuals in the population (Charlesworth and Charlesworth 1987, p. 231; Shaffer 1981, p. 131). Small, isolated wildlife populations are also more susceptible to environmental fluctuations and demographic shifts (Pimm et al. 1988, pp. 757, 773- 775; Shaffer 1981, p. 131), such as reduced reproductive success of individuals and chance disequilibrium of sex ratios. Species tend to have a higher risk of extinction if they occupy a small geographic range and occur at low density (Purvis et al. 2000, p. 1949). The Peruvian plantcutter has experienced a population decline of between 1 and 9 percent in the past 10 years due to habitat loss and this decline is expected to continue in close association with continued habitat loss and degradation (see Factor A) (BLI 2009a, pp. 1-3; BLI 2009g, pp. 1-3; Ridgely and Tudor 1994, p. 18; Snow 2004, p. 69). Extinction risk is heightened in small, declining populations by an increased vulnerability to the loss of genetic variation due to inbreeding depression and genetic drift. This, in turn, compromises a species' ability to adapt genetically to changing environments (Frankham 1996, p. 1507) and reduces fitness, thus increasing extinction risk (Reed and Frankham 2003, pp. 233-234). Once a population is reduced below a certain number of individuals, it tends to rapidly decline towards extinction (Franklin 1980, pp. 147-148; Gilpin and Soule 1986, p. 25; Holsinger 2000, pp. 64-65; Soule 1987, p. 181). Complications arising from the species' small population size are exacerbated by its fragmented distribution. Because remaining habitat patches are small, heavily degraded, and widely separated, the Peruvian plantcutter's current distribution is highly restricted and severely fragmented (BLI 2009a, pp. 2-3; Bridgewater et al. 2003, p. 132; Flanagan et al. in litt. 2009, pp. 1-9; Ridgely and Tudor 1994. p. 18). Habitat fragmentation can cause genetic isolation and heighten the risks to the species associated with short-term genetic viability. A species' small population size, combined with a restricted and fragmented distribution, exacerbates a species' vulnerability to adverse natural events (e.g., genetic, demographic, or environmental) and manmade activities (e.g., land clearing, timber and firewood cutting, and grazing by goats) (Holsinger 2000, pp. 64-65; Primack 1998, pp. 279-308; Young and Clarke 2000, pp. 361-366). Summary of Factor E The Peruvian plantcutter has a small population size that renders it vulnerable to genetic risks that negatively impact the species' viability. The species occurs primarily in two disjunct subpopulations, and remaining habitat is highly fragmented and continues to be altered by human activities (Factor A). Its small population size, combined with its restricted and fragmented range, increases the Peruvian plantcutter's vulnerability to extinction, through demographic or environmental fluctuations. Based on its small population size and fragmented distribution, we have determined that the Peruvian plantcutter is particularly vulnerable to the threat of adverse natural events (e.g., genetic, demographic, or environmental) and human activities (e.g., deforestation, habitat alteration, and infrastructure development) that destroy individuals and their habitat. The genetic and demographic risks associated with small population sizes are exacerbated by ongoing human activities that continue to curtail the species' habitat throughout its range. The species' population has declined and is predicted to continue declining commensurate with ongoing habitat loss (Factor A) and we consider that the risks associated with small population size will continue to impact this species and may accelerate, if habitat destruction continues unabated. Therefore, we find that the species' small population size, in concert with its fragmented distribution and its heightened vulnerability to adverse natural events and manmade activities, are threats to the continued existence of the Peruvian plantcutter throughout its range. Status Determination for the Peruvian Plantcutter The Peruvian plantcutter, a small, herbivorous bird, is endemic to semiarid lowland dry forests of coastal northwest Peru. The species' primary habitat is Prosopis pallida dry forest between 33 and 1, 804 ft (10 and 550 m) above sea level. The species is dependent on year-round availability of high-quality food and is known primarily in two disjunct subpopulations, with several smaller, widely separated sites in the Regions of Piura, Lambayeque, Cajamarca, La Libertad, and Ancash (from north to south). The actual range of the Peruvian plantcutter is smaller than the reported range of 1,892 mi\2\ (4,900 km\2\), given the severely fragmented distribution of the species. The species' population size is estimated to be 500-1,000 individuals. We have carefully assessed the best available scientific and commercial information regarding the past, present, and potential future threats faced by the Peruvian plantcutter and have concluded that there are three primary factors that threaten the continued existence of the Peruvian plantcutter: (1) Habitat destruction, fragmentation, and degradation; (2) limited size and isolation of remaining populations; and (3) inadequate regulatory mechanisms. Human activities that degrade, alter, and destroy habitat are ongoing throughout the Peruvian plantcutter's range. Widespread land conversion to agriculture has removed the vast majority of P. pallida dry forest habitat throughout the range of the Peruvian plantcutter. Peruvian plantcutter habitat continues to be altered by human activities, such as timber and firewood cutting, burning, and grazing, which result in the continued degradation, conversion, and destruction of habitat and reduce the quantity, quality, distribution, and regeneration of remaining dry forest habitat. Current research indicates that narrow endemics, such as the Peruvian plantcutter, are especially susceptible to climate fluctuations, because of the synergistic effect these fluctuations have on declining populations that are also experiencing range reductions due to human activities. Remaining habitat is highly fragmented and disjunct, separated by large areas of converted [[Page 636]] land (e.g., agriculture, urban areas) and sparse vegetation (e.g., Sechura Desert), and excessive rains induce further habitat destruction and prevent regeneration, as people take advantage of better grazing and growing climate conditions. The Peruvian plantcutter's population is small, rendering the species particularly vulnerable to the threat of adverse natural events (e.g., genetic, demographic, or environmental) and human activities (e.g., deforestation and firewood extraction) that destroy individuals and their habitat. Ongoing human activities that cause habitat loss throughout the species' range exacerbate the genetic and demographic risks associated with small population sizes (Factor E). The population has declined 1-9 percent in the past 10 years (see Population Estimates), in association with continued habitat loss (Factor A). Habitat loss was a factor in this species' historical decline (see Historical Range and Distribution)--the Peruvian plantcutter has been extirpated from 11 of its 14 historical sites (see Current Range and Distribution)--and the species is considered to be declining today in association with the continued reduction in habitat (Factors A and E). Moreover, current research indicates that narrow endemics, such as the Peruvian plantcutter, are especially susceptible to climate fluctuations because of the synergistic effect these fluctuations have on declining populations that are also experiencing range reductions due to human activities (Factor A). Despite the species' endangered status in Peru and its occurrence within two protected areas (Factor D), habitat loss and degradation continue throughout the Peruvian plantcutter's habitat (Factor A). Therefore, regulatory mechanisms are either inadequate or ineffective at mitigating the existing threats to the Peruvian plantcutter and its habitat (Factor D). Section 3 of the Act defines an ``endangered species'' as ``any species which is in danger of extinction throughout all or a significant portion of its range'' and a ``threatened species'' as ``any species which is likely to become an endangered species within the foreseeable future throughout all or a significant portion of its range.'' Based on the immediate and ongoing significant threats to the Peruvian plantcutter throughout its entire range, as described above, we determine that the Peruvian plantcutter is in danger of extinction throughout all of its range. Therefore, on the basis of the best available scientific and commercial information, we are proposing to list the Peruvian plantcutter as an endangered species throughout all of its range. V. Royal cinclodes (Cinclodes aricomae) Species Description The royal cinclodes, also known as ``churrete real'' and ``remolinera real,'' is a large-billed ovenbird in the Furnaridae family that is native to high-altitude woodlands of the Bolivian and Peruvian Andes (BLI 2009i, pp. 1-2; del Hoyo et al. 2003, p. 253; InfoNatura 2007, p. 1; Supreme Decree No. 034-2004-AG 2004, p. 27685; Valqui 2000, p. 104). The adult is nearly 8 in (20 cm) in length, with dark chocolate-brown plumage on the upperparts, with a darker crown and a buff-colored area above the eyes. The throat is buff-colored, and the remaining underparts are gray-brown to buff-white. The wings are dark with prominent edging that forms a distinctive wing-bar in flight. The large, dark bill is slightly curved at the tip (BLI 2009i, p. 1). Taxonomy When the species was first taxonomically described, the royal cinclodes was placed in the genus Upucerthia (Carriker 1932, pp. 1-2) and was then transferred to Geositta as a subspecies (Geositta excelsior aricomae) (Vaurie 1980, p. 14). Later, it was transferred to the genus Cinclodes, where it was considered a race or subspecies of the stout-billed cinclodes (Cinclodes excelsior) until recently (BLI 2009i, p. 1; Fjeldsa and Krabbe 1990, pp. 337-338; Vaurie 1980, p. 15). The royal cinclodes is now considered a distinct species (C. aricomae) based on differences in its habitat, morphology, and genetic distance (Chesser 2004, p. 763; del Hoyo et al. 2003, p. 253). Therefore, we accept the species as Cinclodes aricomae, which also follows ITIS (2009, p. 1). Habitat and Life History The royal cinclodes is restricted to moist and mossy habitat amidst the steep, rocky slopes of semihumid Polylepis or Polylepis - Gynoxys woodlands, where the species is found at elevations between 11,483 and 12,092 ft (3,500 and 4,600 m) (BLI 2000, p. 345; BLI 2009i, p. 2; Collar et al. 1992, p. 588; del Hoyo et al. 2003, p. 253). The characteristics of Polylepis habitat were described above as part of the Habitat and Life History of the ash-breasted tit-tyrant (Aucca and Ramsay 2005, p. 1; Chutas et al. 2008, p. 3; De la Via 2004, p. 10; IPNI 2009, p. 1; Kessler 1998, p. 1; Kessler and Schmidt-Lebuhn 2006, pp. 1-2; Purcell et al. 2004, p. 455). The royal cinclodes prefers dense woodlands (BLI 2000, p. 345; BLI 2009i, p. 2; Collar et al. 1992, p. 588; del Hoyo et al. 2003, p. 253), with more closed canopies that provide habitat for more lush moss growth (Engblom et al. 2002, p. 57). The moss-laden vegetation and shaded understory harbor a rich diversity of insects, making good feeding grounds for insectivorous birds (De la Via 2004, p. 10) such as the royal cinclodes (del Hoyo et al. 2003, p. 253; Engblom et al. 2002, p. 57). In Bolivia, the royal cinclodes has been observed only in P. pepei forests, but it is found amongst a greater variety of Polylepis species in Peru (Chutas et al. 2008, p. 16; I. Gomez, National Museum of National History-Ornithology Section- Bolivian Fauna Collection, La Paz, Bolivia, e-mail to Division of Scientific Authority, in litt. December 4, 2007, p. 1). Information on the ecology and breeding behavior of royal cinclodes is limited. The species' feeding territory ranges from 7 to 10 ac (3 to 4 ha) (del Hoyo et al. 2003, p. 253; Engblom et al. 2002, p. 57). Breeding pairs may occupy smaller, 2.5-ac (2-ha) territories (Chutas 2007, p. 7). The royal cinclodes is described as ``nervous'' and is easily disturbed by humans (Engblom et al. 2002, p. 57). The breeding season probably begins in December, but territorialism among pairs can be seen in austral winter (June-August) (BLI 2000, p. 345; del Hoyo et al. 2003, p. 253). Cinclodes species construct burrows or use natural cavities, crevices, or rodent burrows for nesting (Fjeldsa and Krabbe 1990, p. 337; Vaurie 1980, pp. 30, 34). The royal cinclodes' clutch size may be similar to that of the closely-related stout-billed cinclodes (C. excelsior), which, according to Graves and Arango (1988, p. 252), is two eggs per clutch. Its diet consists primarily of invertebrates, sometimes seeds, and occasionally small vertebrates (small frogs) (del Hoyo et al. 2003, p. 253). The royal cinclodes forages, solitary or in pairs, by probing through moss and debris on the forest floor (BLI 2000, p. 345; Collar et al. 1992, p. 589; del Hoyo et al. 2003, p. 253; Fjeldsa 2002b, p. 9). This ground-feeding strategy generally requires a relatively large territory and may also facilitate interbreeding amongst groups located on adjoining mountain peaks when the species likely descends the mountains during periods of snow cover (Engblom et al. 2002, p. 57). Historical Range and Distribution The royal cinclodes may once have been locally common and distributed [[Page 637]] across most of central to southern Peru and into the Bolivian highlands, in once-contiguous expanses of Polylepis forests above 9,843 ft (3,000 m) (BLI 2000, p. 345; BLI 2009i, p. 1; Fjeldsa 2002a, pp. 111-112, 115; Herzog et al. 2002, p. 94; Kessler 2002, pp. 97-101). Polylepis woodlands are now restricted to elevations of 11,483 to 16,404 ft (3,500 to 5,000 m) (Fjeldsa 1992, p. 10). As discussed above for the Historical Range and Distribution of the ash-breasted tit- tyrant, researchers consider human activity to be the primary cause for historical habitat decline and resultant decrease in species richness (Fjeldsa and Kessler 1996, Kessler 1995a, b, and L[aelig]gaard 1992, as cited in Fjeldsa 2002a, p. 112; Fjeldsa 2002a, p. 116; Herzog et al. 2002, p. 94; Kessler 2002, pp. 97-101; Kessler and Herzog 1998, pp. 50- 51). The royal cinclodes may have been extirpated from its type locality (Aricoma Pass, Puno), and possibly throughout the entire Puno Region, where Polylepis forest no longer occurs exists (Collar et al. 1992, p. 589; Engblom et al. 2002, p. 57) (see Population Estimates). It is estimated that between 2-3 percent and 10 percent of the original forest cover still remains in Peru and Bolivia, respectively (BLI 2009i, p. 1; Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113) (see Factor A). Of this amount, less than 1 percent of the remaining woodlands occur in humid areas, where Polylepis denser stands occur (Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113) and which are preferred by the royal cinclodes (del Hoyo et al. 2003, p. 253; Engblom et al. 2002, p. 57). The royal cinclodes was initially discovered in Bolivia in 1876, but was not observed there again until recently (BLI 2009i, p. 2; Hirshfeld 2007, p. 198) (see Current Range and Distribution). Current Range and Distribution The royal cinclodes is restricted to moist and mossy habitat amidst the steep rocky slopes of semihumid Polylepis or Polylepis - Gynoxys woodlands, where the species is found at elevations between 11,483 and 12,092 ft (3,500 and 4,600 m) (BLI 2000, p. 345; BLI 2009i, p. 2; Collar et al. 1992, p. 588; del Hoyo et al. 2003, p. 253). The current range of the species is approximately 1,042 mi\2\ (2,700 km\2\) (BLI 2009i, p. 1), which is an overestimate of the actual range (as discussed under the Current Range and Distribution of the ash-breasted tit-tyrant) (BLI 2000, pp. 22, 27), given the fragmented nature of the species' remaining habitat (BLI 20091, p. 1; Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113). The royal cinclodes was only rediscovered in Bolivia within the last decade, after more than 100 years of not being observed there (BLI 2009i, p. 2; Hirshfeld 2007, p. 198). Within the last 15 years, royal cinclodes has been observed in Peru's Runtacocha highlands (Apurimac), Pariahuanca Valley (Junin), and Cordillera Vilcanota (Cusco), and in Bolivia's Cordillera Apolobamba and the Cordillera Real (including Ilampu Valley, Sanja Pampa, and Cordillera de La Paz), all in the Bolivian Department of La Paz (BLI 2007, pp. 1-2; BLI 2009i, pp. 1-2; del Hoyo et al. 2003, p. 253; Engblom et al. 2002, p. 57; Hirshfeld 2007, p. 198; InfoNatura 2007, p. 1; Valqui 2000, p. 104). Population Estimates Population information is presented first on the range-country level and then in terms of a global population estimate. The range country estimates begin with Peru, where the majority of the population resides. Local population estimate, Peru: In 1990, the global population was estimated to be 100-150 individuals (Fjeldsa and Krabbe 1990, p. 338). This number represents the estimated Peruvian population because the royal cinclodes was only thought to be extant in Peru at the time of this estimate (BLI 2009i, p. 2; Hirshfeld 2007, p. 198). Chutas (2007, p. 8) reported an estimated 189 birds located within four disjunct Polylepis forest patches in Peru, with a combined area of 1,554 ac (629 ha). This estimate included 116 birds and 30 birds in Cordilleras Vilcanota and Vilcabamba, respectively (Cusco); 2 birds in Cordillera de Carabaya (Puno); and 41 birds in ``Cordillera del Apurimac'' (Runtacocha highlands in Apurimac) (Chutas 2007, pp. 4, 8). Subpopulations at four locations in the Cordillera Vilcanota contain as few as 1-4 individuals (BLI 2008, p. 2). In the Puno Region of Peru, it is unclear whether a viable population of royal cinclodes remains. The royal cinclodes was first observed in Puno in 1930 (Fjeldsa and Krabbe 1990, p. 338) and has continued to be reported there in general terms (BLI 2007, pp. 1-2; BLI 2009i, pp. 1-2; Collar et al. 1992, p. 588; del Hoyo 2003, p. 253). However, based on habitat availability, InfoNatura (2007, p. 1) predicted that the royal cinclodes does not occur in Puno because the habitat no longer exists there. Indeed, only two royal cinclodes individuals have been reported in the Puno Region (Cordillera de Carabaya) in recent decades (Chutas 2007, pp. 4, 8). There are no other recent observations of the royal cinclodes in Puno (BLI 2009i, p. 2; del Hoyo 2003, p. 283; Engblom et al. 2002, p. 57). The species is believed to be extirpated from its type locality (Collar et al. 1992, p. 589; Engblom et al. 2002, p. 57). Local population estimate, Bolivia: The species' current range is more widespread in Bolivia than previously understood. The royal cinclodes had not been observed in Bolivia for more than one century, when it was rediscovered there in 1997 (BLI 2009i, p. 2; Hirshfeld 2007, p. 198). Recent surveys in La Paz Department found at least 13 localities (8 in Cordillera Apolobamba and 5 in Cordillera La Paz) (BLI 2009i, p. 1). Although BirdLife International reports an estimated population size of 50-70 royal cinclodes in Bolivia (Gomez, in litt. 2003, 2008, as cited in BLI 2009i, p. 2), recent surveys indicate that the estimate may be smaller. As discussed above for the local population estimate of the ash-breasted tit-tyrant in Bolivia, Gomez (in litt. 2007, p. 1) conducted intensive studies in Bolivia. From this research, the presence of 1-8 royal cinclodes in each of 30 forest patches was inferred or observed. Thus, they estimated that the royal cinclodes population in Bolivia totals approximately 30 birds. Researchers add that, because the royal cinclodes does not always respond to tape- playbacks, these numbers may underestimate the actual population size (Gomez in litt. 2007, p. 1). Global population estimate: In 1990, the global population of the royal cinclodes was estimated to be 100-150 individuals (Fjeldsa and Krabbe 1990, p. 338). Since at least 2000, BirdLife International has placed this species in the population category of between 50 and 249 individuals (BLI 2000, p. 345). In 2002, Engblom et al. (2002, p. 57) estimated a total population size of up to 250 pairs of birds. This amount far exceeds any previous estimates and has not been confirmed by BirdLife International (BLI 2009i, p. 1). In 2003, the global population was once again reported to include only ``a few hundred individuals'' (del Hoyo et al. 2003, p. 253). Based on recent observations in both countries, there are approximately 189 birds in Peru and 50-70 in Bolivia, totaling 239-259 individuals. Recognizing that the royal cinclodes does not always respond to tape-playbacks, this could be an underestimate of the population size (Gomez in litt. 2007, p. 1). While the species continues to be categorized by BirdLife International as having an estimated population between 50-249 individuals (BLI 2007, p. 1; BLI 2009i, p. 1; Fjeldsa 2002b, p. 9; Hirshfeld 2007, p. 198), it is possible that the recent [[Page 638]] observations in Bolivia will lead to a revision of the species' population estimate (BLI 2009i, p. 1). It should be noted that the total population size, which includes immature individuals, is not an accurate reflection of the species' effective population size (the number of breeding individuals that contribute to the next generation) (Shaffer 1981, pp. 132-133; Soule 1980, pp. 160-162). The IUCN estimated that the entire royal cinclodes population contains fewer than 250 mature individuals and no more than 50 mature individuals in any subpopulation (BLI 2008, p. 1; IUCN 2001, pp. 8-12). However, population estimates are incomplete for several of the known localities, and the subpopulation structure and the extent of interbreeding amongst the various localities are unknown. The species' territory ranges from 7 to 10 ac (3 to 4 ha), and its habitat is fragmented, dispersed and sparse (del Hoyo et al. 2003, p. 253; Engblom et al. 2002, p. 57). However, there is no information to indicate the distance that this species is capable of or likely to travel between localities. Engblom et al. (2002, p. 57) noted that gene flow between localities likely occurs when the species descends the mountains to forage in the valleys during periods of snow cover at the higher altitudes such that interbreeding may occur at least among localities with shared valleys. This suggests that the species does not breed as a single population. However, there is insufficient information to determine the extent to which this species functions as genetically isolated subpopulations. The species has experienced a population decline of between 30 and 49 percent in the past 10 years, and this rate of decline is predicted to continue (BLI 2009i, pp. 1, 5). The population is considered to be declining in close association with continued habitat loss and degradation (BLI 2009i, p. 6) (see Factors A and E). Conservation Status The royal cinclodes is considered ``critically endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276854). The IUCN considers the royal cinclodes to be ``Critically Endangered'' due to its extremely small population, which consists of ``tiny'' subpopulations that are severely fragmented and dependent upon a rapidly declining habitat (BLI 2007, p. 1; BLI 2009i, p. 1). The royal cinclodes occurs within the Peruvian protected area of Santuario Historico Machu Picchu, in Cusco (BLI 2009h, p. 1; BLI 2009i, p. 6; Chutas et al. 2008, p. 16). In La Paz Department, Bolivia, the species is found in Parque Nacional y area Natural de Manejo Integrado Madidi, Parque Nacional y area Natural de Manejo Integrado Cotapata, and the co-located protected areas of Reserva Nacional de Fauna de Apolobamba, area Natural de Manejo Integrado de Apolobamba, and Reserva de la Biosfera de Apolobamba (BLI 2009a, p. 1; BLI 2009b, p. 1; BLI 2009c, p. 1; BLI 2009d, p. 1; BLI 2009i, p. 6; Chutas et al. 2008, p. 16). Summary of Factors Affecting the Royal Cinclodes A. The Present or Threatened Destruction, Modification, or Curtailment of the Species' Habitat or Range The royal cinclodes is restricted to high-elevation (11,483-12,092 ft (3,500-4,600 m)), moist, moss-laden areas of semihumid Polylepis or Polylepis - Gynoxys woodlands (BLI 2000, p. 345; BLI 2009i, p. 2; Collar et al. 1992, p. 588; del Hoyo et al. 2003, p. 253). As described more fully for the ash-breasted tit-tyrant (Factor A), Polylepis habitat is characterized as a threatened woodland ecosystem on national, regional, and global levels (BLI 2009a, p. 2; Purcell et al. 2004, p. 457; Renison et al. 2005, as cited in Lloyd 2009, p. 10), with several Polylepis species within the royal cinclodes' range considered to be ``Vulnerable,'' according to the IUCN (WCMC 1998a, p. 1; WCMC 1998b, p. 1). Polylepis woodlands are dispersed and sparse, with an estimated remaining area of 386 mi\2\ (1,000 km\2\) in Peru and 1,931 mi\2\ (5,000 km\2\) in Bolivia (Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113). Within the remaining Polylepis woodlands, the royal cinclodes' range is approximately 1,042 mi\2\ (2,700 km\2\) (BLI 2009i, p. 1). Less than 1 percent of the remaining woodlands occur in humid areas, where denser stands occur (Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113). The optimal habitat for the royal cinclodes is dense woodlands, with a closed canopy that supports the best foraging habitat (shady, moss-laden vegetation) for this and other insectivorous birds (see Habitat and Life History) (De la Via 2004, p. 10; del Hoyo et al. 2003, p. 253; Engblom et al. 2002, p. 57). Habitat loss, conversion, and degradation occur throughout the royal cinclodes' range and have been attributed to human activities (a full description of which is provided above as part of the Factor A analysis for the ash-breasted tit-tyrant). These activities include: (1) Clearcutting and uncontrolled burning for agriculture and pastureland for domesticated animals, all of which contributes to loss of understory moss cover, soil erosion, and degradation, which prevent woodland regeneration (BLI 2009a, p. 2; BLI 2009b, p. 2; BLI 2009c, p. 2; BLI 2009d, p. 2; BLI 2009e, p. 3; BLI 2009f, p. 1; BLI 2009g, p. 1; BLI 2009h, p. 4; BLI 2009i, pp. 2, 6; Engblom et al. 2002, p. 56; Fjeldsa 2002a, pp. 112, 120; Fjeldsa 2002b, p. 8; Jameson and Ramsay 2007, p. 42; Purcell et al. 2004, p. 458; WCMC 1998a, p. 1; WCMC 1998b, p. 1); (2) Extractive activities, such as wood and timber, for local and commercial-scale uses, including firewood and construction (Aucca and Ramsay 2005, p. 287; BLI 2009a, p. 2; BLI 2009b, p. 2; BLI 2009c, p. 2; BLI 2009d, p. 2; BLI 2009e, p. 3; BLI 2009g, p. 1; BLI 2009i, p. 2; Engblom 2000, p. 1; p. 2; Engblom et al. 2002, p. 56; Purcell et al. 2004, p. 458; WCMC 1998a, p. 1); (3) Human encroachment, including tourism and industrialization projects, which puts greater demand on natural resources, spurs additional habitat destruction as arable land becomes scarce, and increases infrastructure development that further facilitates encroachment (BLI 2009b, p. 2; BLI 2009d, p. 2; Hensen 2002, p. 199; Purcell and Brelsford, 2004, pp. 156-157; Purcell et al. 2004, pp. 458- 459); and (4) Unpredictable climate fluctuations that exacerbate the effects of habitat fragmentation (Jetz et al. 2007, pp. 1211, 1213; Mora et al. 2007, p. 1027). These habitat-altering activities are ongoing throughout the royal cinclodes' range, including the Apurimac (BLI 2009g, p. 1) and Cusco Regions (BLI 2009e, p. 1; BLI 2009f, p. 1; BLI 2009h, p. 1) in Peru and also in the Bolivian Department of La Paz (BLI 2009a, p. 1; BLI 2009b, p. 1; BLI 2009c, pp. 1; BLI 2009d, p. 1; Hensen 2002, p. 199; Purcell and Brelsford 2004, p. 157; Purcell et al. 2004, pp. 458-459). A combination of urbanization, road building, and industrialization projects (such as construction of hydroelectric power stations) in the Bolivian Department of La Paz have resulted in a nearly 40 percent loss of the forest cover between 1991 and 2003 alone; at this rate it is predicted that the remaining Polylepis forest in La Paz will be extirpated within the next 30 years (Purcell and Brelsford 2004, pp. 156-157). Community-based Polylepis conservation programs have been under way in Peru and Bolivia since 2004 (Gomez in litt. 2003, 2008, as cited in BLI 2009i, p. 2; MacLennan 2009, p. 2), [[Page 639]] and have focused on known sites for the royal cinclodes (BLI 2009i, p. 2), including Cordilleras Vilcanota and Vilcabamba, and highlands of the Apurimac Region (Aucca and Ramsey 2005, p. 287; ECOAN n.d., p. 1; Lloyd 2009, p. 10). These programs confront the main causes of Polylepis woodland loss--burning, grazing, and wood-cutting (Aucca and Ramsay 2005, pp. 187-288; BLI 2009i, p. 2; ECOAN n.d., p. 1; Engblom et al. 2002, p. 56; Gomez in litt. 2003, 2008, as cited in BLI 2009i, p. 2; Lloyd 2009, p. 10; MacLennan 2009, p. 2) --and are more fully described above as part of the Factor A analysis for the ash-breasted tit-tyrant (Aucca and Ramsay 2005, p. 287; Engblom et al. 2002, p. 56; MacLennan 2009, p. 2). While the Polylepis conservation programs foster local, sustainable use of resources (Aucca and Ramsay 2005, p. 287; ECOAN n.d., p. 1; Engblom et al. 2002, p. 56), commercial-scale activities, such as clearcutting, logging, tourism, and infrastructure development, that are ongoing throughout this species' range, alter otherwise sustainable resource use practices (Aucca and Ramsay 2005, p. 287; Engblom 2000, p. 2; Engblom et al. 2002, p. 56; MacLennan 2009, p. 2; Purcell and Brelsford 2004, pp. 156-157; Purcell et al. 2004, pp. 458-459; WCMC 1998a, p. 1). Tourism and human encroachment are particularly problematic for the royal cinclodes, which is described as a ``nervous'' species that is easily disturbed by humans (Engblom et al. 2002, p. 57). In the Cordillera de Vilcanota (Cusco, Peru), where a large portion of the known royal cinclodes population occurs (116 birds were observed there, out of 189 total birds observed in 4 study sites in Peru) (Chutas 2007, pp. 4, 8), Polylepis woodland habitat is highly fragmented and degraded. As described more fully for the ash-breasted tit-tyrant (Factor A), recent research indicated: (1) That four forest patches in the Cordillera de Vilcanota disappeared completely in the last half a century, that the size of remaining Polylepis remnants is small (with a mean patch size of 7.4 ac (3 ha)) (Jameson and Ramsay 2007, p. 42), and that many patches are less than 2.5 ac (1 ha) (Lloyd and Marsden in press, as cited in Lloyd 2008, p. 532); (2) Ten percent of the remaining forest patches showed a decline in forest density (Jameson and Ramsay 2007, p. 42); and (3) There were no indications of forest regeneration within the study area. Thus, forest patches in Cordillera Vilcanota are at or below the minimum area required for the royal cinclodes to obtain food, given that the ground-feeding strategy used by the royal cinclodes generally requires a relatively large territory, from 7 to 10 ac (3 to 4 ha) (del Hoyo et al. 2003, p. 253; Engblom et al. 2002, p. 57). Because the moist, moss-covered woodlands that provide optimal foraging habitat for insectivorous birds (De la Via 2004, p. 10), and which this ground- feeding species prefers (del Hoyo et al. 2003, p. 253; Engblom et al. 2002, p. 57), require a closed canopy, degradation of the royal cinclodes habitat has serious consequences for this species. Reduction of forest density (or, decreased canopy cover) increases desiccation of the moist and mossy ground cover, which, in turn, reduces foraging microhabitats for the species (Engblom et al. 2002, p. 57). Lack of Polylepis forest regeneration during nearly 50 years underscores the ramifications of continued burning and clearing to maintain pastures and farmland, which are prevalent activities throughout the royal cinclodes' range (BLI 2009a, p. 2; BLI 2009b, p. 2; BLI 2009c, p. 2; BLI 2009d, p. 2; BLI 2009e, p. 3; BLI 2009f, p. 1; BLI 2009g, p. 1; BLI 2009h, p. 4; BLI 2009i, p. 2; Engblom et al. 2002, p. 56; Fjeldsa 2002a, pp. 112, 120; Fjeldsa 2002b, p. 8; Purcell et al. 2004, p. 458; WCMC 1998a, p. 1; WCMC 1998b, p. 1). These habitat- altering activities are considered to be key factors preventing regeneration of Polylepis woodlands (Fjeldsa 2002a, p. 112, 120; Fjeldsa 2002b, p. 8) and are factors in the historical decline of Polylepis -dependent bird species, including the royal cinclodes (BLI 2009i, p. 6; Fjeldsa and Kessler 1996, Kessler 1995a, b, and L[aelig]gaard 1992, as cited in Fjeldsa 2002a, p. 112; Fjeldsa 2002a, p. 116; Herzog et al. 2002, p. 94; Kessler 2002, pp. 97-101; Kessler and Herzog 1998, pp. 50-51). The royal cinclodes may once have been locally common and distributed across most of central to southern Peru and into the Bolivian highlands, in once contiguous expanses of Polylepis forests (BLI 2000, p. 345; BLI 2009i, p. 1; Fjeldsa 2002a, pp. 111-112, 115). The royal cinclodes' population size is considered to be declining in close association with continued habitat loss and degradation (BLI 2007, p. 1; BLI 2008, p. 1; BLI 2009i, p. 6). The species may have been extirpated from its type locality (Aricoma Pass, Puno), where Polylepis forest no longer occurs, and a search for the species in 1987 resulted in no observations of the royal cinclodes (Collar et al. 1992, p. 589; Engblom 2002, p. 57). Based on habitat availability, the royal cinclodes is not predicted to occur in Puno because the habitat no longer exists there (InfoNatura 2007, p. 1), and only two birds have been observed there in recent years (Chutas 2007, pp. 4, 8). Therefore, further habitat loss will continue to impact the species' already small population size (see Factor E). Royal cinclodes are also impacted by unpredictable climate fluctuations, which are more fully described under the Factor A analysis of the ash-breasted tit-tyrant and are summarized here. Peru is subject to unpredictable climate fluctuations that exacerbate the effects of habitat fragmentation, such as those that are related to the El Ni[ntilde]o Southern Oscillation (ENSO). Changes in weather patterns, such as ENSO cycles (El Ni[ntilde]o and La Ni[ntilde]a events), tend to increase precipitation in normally dry areas, and decrease precipitation in normally wet areas (Holmgren et al. 2001, p. 89; TAO Project n.d., p. 1), exacerbating the effects of habitat fragmentation on the decline of a species (England 2000, p. 86; Holmgren et al. 2001, p. 89; Jetz et al. 2007, pp. 1211, 1213; Mora et al. 2007, p. 1027; Parmesan and Mathews 2005, p. 334; Plumart 2007, pp. 1-2; Timmermann 1999, p. 694), especially for narrow endemics (Jetz et al. 2007, p. 1213) such as the royal cinclodes (see also Factor E). ENSO cycles strongly influence the growth of Polylepis species (Christie et al. 2008, p. 1) by altering the Polylepis species' age structure and mortality, especially where woodlands have undergone disturbance, such as fire and grazing (Villalba and Veblen 1997, pp. 121-123; Villalba and Veblen 1998, pp. 2624, 2637). ENSO cycles may have already accelerated the fire cycle (Block and Richter 2007, p. 1; Power et al. 2007, pp. 897-898), which is a key factor preventing Polylepis regeneration (Fjeldsa 2002a, p. 112, 120; Fjeldsa 2002b, p. 8) because Polylepis species recover poorly following a fire (Cierjacks et al. 2007, p. 176). ENSO cycles are ongoing, having occurred several times within the last decade (NWS 2009, p. 2), and evidence suggests that ENSO cycles have already increased in periodicity and severity (Richter 2005, pp. 24-25; Timmermann 1999, p. 694), which will exacerbate the negative impacts of habitat destruction on a species. It is predicted that, by 2050, approximately 3 to 15 percent of the royal cinclodes' current remaining range is likely to be unsuitable for this species due to climate change and, by 2100, it is predicted that about 8 to 18 percent of the species' range is likely to be lost as a direct result of global climate change (Jetz et al. 2007, Supplementary Table 2, p. 89). [[Page 640]] Human activities that alter the species' habitat are also ongoing within protected areas, including Santuario Historico Machu Picchu (in Peru) (BLI 2009h, p. 4), and Parque Nacional y area Natural de Manejo Integrado Madidi, Parque Nacional y area Natural de Manejo Integrado Cotapata, and the co-located protected areas of Reserva Nacional de Fauna de Apolobamba, area Natural de Manejo Integrado de Apolobamba, and Reserva de la Biosfera de Apolobamba (in Bolivia) (BLI 2009a, p. 2; BLI 2009b, p. 2; BLI 2009c, p. 2; BLI 2009d, p. 5). Ongoing habitat destruction and alteration within protected areas, including clearing and human encroachment, is further discussed under Factor D. Summary of Factor A Polylepis habitat throughout the royal cinclodes' range has been and continues to be altered and destroyed as a result of human activities, including clearcutting and burning for agriculture, grazing lands, and industrialization; extractive activities, including firewood and timber extraction; and human encroachment and concomitant increased pressure on natural resources. An estimated 1 percent of the once- extensive dense Polylepis woodlands remains, and other remaining Polylepis woodlands are fragmented and degraded. The royal cinclodes occupies an area of approximately 1,042 mi\2\ (2,700 km\2\), and is particularly vulnerable to reduction in forest cover, because the moist habitats that serve as their feeding grounds quickly dry out as the forest canopy diminishes. Researchers estimate that the royal cinclodes territories are 7-10 ac (3-4 ha). In Cordillera Vilcanota (Cusco, Peru), where a large concentration of the royal cinclodes individuals were observed in 2007, the average size of forest fragments just meets the lower threshold of the species' ecological requirements. While the species' range is more widespread in Bolivia than previously understood, ongoing and accelerated habitat destruction of the remaining Polylepis forest fragments in Peru and Bolivia continues to reduce the quantity, quality, distribution, and regeneration of remaining patches. In the Administrative Region of Puno, Peru, habitat loss may have led to extirpation of the species from its type locality and the species may no longer be viable in that Region due to habitat loss. Current research indicates that climate fluctuations exacerbate the effects of habitat loss on species, especially for narrow endemics such as the royal cinclodes that are already undergoing range reduction due to human activities. Historical decline in habitat availability is attributed to the same human activities that are causing habitat loss today, and climate models predict that this species' habitat will continue to decline. In addition, the royal cinclodes is ``nervous'' around humans, such that human encroachment is a particular problem. Human activities that degrade, alter, and destroy habitat are ongoing throughout the species' range, including within protected areas (see also Factor D). Experts consider the species' population decline to be commensurate with the declining habitat (Factor E). Therefore, we find that destruction and modification of habitat are threats to the continued existence of the royal cinclodes throughout its range. B. Overutilization for Commercial, Recreational, Scientific, or Educational Purposes We are not aware of any information currently available that indicates that overutilization of the royal cinclodes for commercial, recreational, scientific, or educational purposes has occurred or is occurring at this time. As a result, we are not considering overutilization to be a threat to the continued existence of the royal cinclodes. C. Disease or Predation We are not aware of any scientific or commercial information that indicate disease or predation poses a threat to this species. As a result, we are not considering disease or predation to be a threat to the continued existence of the royal cinclodes. D. Inadequacy of Existing Regulatory Mechanisms Existing regulatory mechanisms within Peru and Bolivia that have the potential to confer protection to the royal cinclodes or its habitat are analyzed on a country-by-country basis, beginning with Peru. Peru: The royal cinclodes is considered ``critically endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276855). This Decree prohibits hunting, take, transport, and trade of protected species, except as permitted by regulation. As hunting, take, transport, and trade do not currently threaten the royal cinclodes, this regulation does not mitigate any current threats to this species. Peru has several categories of national habitat protection, which were described above as part of the Factor D analysis for the ash- breasted tit-tyrant (BLI 2008, p. 1; IUCN 1994, p. 2; Rodriguez and Young 2000, p. 330). Protected areas have been established through regulation at one site occupied by the royal cinclodes in Peru: Santuario Historico Machu Picchu (Cusco, Peru) (BLI 2009h, p. 4). Within the sanctuary, resources are supposed to be managed for conservation (Rodriguez and Young 2000, p. 330). However, habitat destruction and alteration, including burning to maintain pastures for grazing, are ongoing within Santuario Historico Machu Picchu, preventing the regeneration of the woodlands (BLI 2009h, p. 4; Engblom et al. 2002, p. 58). Therefore, the occurrence of the royal cinclodes within protected areas in Peru does not protect the species, nor does it mitigate the threats to the species from ongoing habitat loss (Factor A) and concomitant population decline (Factor E). Bolivia: The 1975 Law on Wildlife, National Parks, Hunting, and Fishing (Decree Law No. 12,301 1975, pp. 1-34; eLAW 2003, p. 2), was described above as part of the Factor D analysis for the ash-breasted tit-tyrant. This law designates national protection for all wildlife. However, there is no information as to the actual protections this confers to the species itself or its habitat, and ongoing habitat destruction throughout the species' range indicates that this law does not protect the species nor does it mitigate the threat to the species from ongoing habitat loss (Factor A) and concomitant population decline within Bolivia (Factor E). Environmental Law No. 1333 (eLAW 2003, p. 1; Law No. 1,333 1992, pp. 1-26), was signed in 1992 to protect and conserve the environment. However, we are not aware that any specific legislation needed to implement these laws has been passed (eLAW 2003, p. 1). Therefore, this law does not protect the species, nor does it mitigate the threats to the species from ongoing habitat loss (Factor A) and concomitant population decline (Factor E). Various levels of habitat protection in Bolivia were described above as part of the Factor D analysis for the ash-breasted tit-tyrant (eLAW 2003, p. 3; Supreme Decree No. 24,781 1997, p. 3). The royal cinclodes occurs within several protected areas in the Department of La Paz, Bolivia: Parque Nacional y area Natural de Manejo Integrado Madidi, Parque Nacional y area Natural de Manejo Integrado Cotapata, and the co-located protected areas of Reserva Nacional de Fauna de Apolobamba, area Natural de Manejo Integrado de Apolobamba, and Reserva de la Biosfera de Apolobamba (Auza and Hennessey 2005, p. 81; BLI 2009a, p. 1; BLI 2009b, p. 1; BLI 2009c, p. 1; BLI 2009d, p. 1). Within Parque [[Page 641]] Nacional y area Natural de Manejo Integrado Madidi, habitat destruction is caused by timber harvest used for construction, wood collection for firewood, and burning that often goes out of control to maintain pastures (BLI 2009a, p. 2; WCMC 1998a, p. 1). In addition, one of the most transited highways in the country is located a short distance from the Parque Nacional y area Natural de Manejo Integrado Cotapata; firewood collection and grazing also occur within the protected area (BLI 2009b, p. 2; BLI 2009c, p. 2). Within the Apolobamba protected areas, uncontrolled clearing, extensive agriculture, grazing, and tourism are ongoing (Auza and Hennessey 2005, p. 81; BLI 2009d, p. 5). Therefore, the occurrence of the royal cinclodes within protected areas in Bolivia does not protect the species, nor does it mitigate the threats to the species from ongoing habitat loss (Factor A) and concomitant population decline (Factor E). Summary of Factor D Peru and Bolivia have enacted various laws and regulatory mechanisms to protect and manage wildlife and their habitats. The royal cinclodes is ``critically endangered'' under Peruvian law and occurs within several protected areas in Peru and Bolivia. As discussed under Factor A, the royal cinclodes requires dense woodlands, which has been reduced by an estimated 99 percent in Peru and Bolivia. The remaining habitat for the royal cinclodes is fragmented and degraded. Habitat throughout the species' range has been and continues to be altered as a result of human activities, including clearcutting and burning for agriculture, grazing lands, and industrialization; extractive activities, including, firewood, timber, and mineral extraction; and human encroachment and concomitant increased pressure on natural resources. These activities are ongoing within protected areas and despite the species' critically endangered status in Peru, indicating that the laws governing wildlife and habitat protection in both countries are either inadequate or inadequately enforced to protect the species or to mitigate ongoing habitat loss (Factor A) and population declines (Factor E). Therefore, we find that the existing regulatory mechanisms are inadequate to mitigate the current threats to the continued existence of the royal cinclodes throughout its range. E. Other Natural or Manmade Factors Affecting the Continued Existence of the Species An additional factor that affects the continued existence of the royal cinclodes is the species' small population size. Based on recent observations in Peru and Bolivia, the total population is between 239 and 259 individuals (Chutas 2007, pp. 4, 8; Gomez in litt. 2007, p. 1) (see Population Estimates), and BirdLife International characterizes the species as having an ``extremely small population'' size (BLI 2000, p. 345; BLI 2009i, p. 1). Although there is insufficient information to fully understand gene flow within this species (see Population Estimates), Engblom et al. (2002, p. 57) noted that the royal cinclodes may descend the mountains to forage in the valleys during periods of snow cover at the higher altitudes. Thus, interbreeding may occur at least among localities with shared valleys, but there is insufficient information to determine that the species breeds as a single population. Moreover, the total population size, which includes immature individuals, is not an accurate reflection of the species' effective population size (the number of breeding individuals that contribute to the next generation) (Shaffer 1981, pp. 132-133; Soule 1980, pp. 160-162). Therefore, 239-259 is an overestimate of the species' effective population size. Small population size renders species vulnerable to genetic risks that can have individual or population-level genetic consequences, such as inbreeding depression, loss of genetic variation, and accumulation of new mutations. These genetic problems may affect the species' viability by increasing its susceptibility to demographic shifts or environmental fluctuations, as described above in the Factor E analysis for the ash-breasted tit-tyrant (Charlesworth and Charlesworth 1987, p. 238; Pimm et al. 1988, pp. 757, 773-775; Shaffer 1981, p. 131). Small population size leads to a higher risk of extinction and, once a population is reduced below a certain number of individuals, it tends to rapidly decline towards extinction (Frankham 1996, p. 1507; Franklin 1980, pp. 147-148; Gilpin and Soule 1986, p. 25; Holsinger 2000, pp. 64-65; Purvis et al. 2000, p. 1949; Reed and Frankham 2003, pp. 233-234; Soule 1987, p. 181). If further research indicates that interbreeding does not between subpopulations, this would heighten the risks to the species associated with short-term genetic viability. Complications arising from the species' small population size are exacerbated by the species' fragmented distribution. The royal cinclodes is currently restricted to high-elevation, moist, moss-laden patches of semihumid woodlands in Peru and Bolivia (BLI 2009i, p. 6) (Factor A). Fjeldsa and Kessler (1996, as cited in Fjeldsa 2002a, p. 113). Remaining Polylepis woodlands are highly fragmented and degraded, and it is estimated that only 1 percent of the dense woodlands preferred by the species remain (del Hoyo et al. 2003, p. 253; Engblom et al. 2002, p. 57) (see Habitat and Life History and Historical Distribution). Therefore, the species' current range is restricted and severely fragmented (BLI 2000, p. 345; BLI 2009i, pp. 1-2; Collar et al. 1992, p. 588; del Hoyo et al. 2003, p. 253). Habitat fragmentation can cause genetic isolation and heighten the risks to the species associated with short-term genetic viability. The royal cinclodes has undergone a population decline between 30 and 49 percent in the past 10 years, in close association with the continued loss and degradation of the Polylepis forest (BLI 2009i, p. 6) (Factor A). The species' small population size, combined with its restricted and severely fragmented range, increases the species' vulnerability to adverse natural events and manmade activities that destroy individuals and their habitat (Holsinger 2000, pp. 64-65; Primack 1998, pp. 279-308; Young and Clarke 2000, pp. 361-366). Summary of Factor E The royal cinclodes has a small population size that renders it vulnerable to genetic risks that negatively impact the species' long- term viability, and possibly its short-term viability. The species has a restricted range and occurs in highly fragmented habitat that continues to undergo degradation and curtailment due to human activities (Factor A). The restricted and fragmented range, as well as the small population size, increases the species' vulnerability to extinction through demographic or environmental fluctuations. Based on its small population size and fragmented distribution, we have determined that the royal cinclodes is particularly vulnerable to the threat of adverse natural events (e.g., genetic, demographic, or environmental) and human activities (e.g., deforestation, habitat alteration, and infrastructure development) that destroy individuals and their habitat. The genetic and [[Page 642]] demographic risks associated with small population sizes are exacerbated by ongoing human activities that continue to curtail the species' habitat throughout its range. The species' population has declined and is predicted to continue declining commensurate with ongoing habitat loss (Factor A). Therefore, we find that the species' small population size, in concert with its fragmented distribution and its heightened vulnerability to adverse natural events and manmade activities, are threats to the continued existence of the royal cinclodes throughout its range. Status Determination for the Royal Cinclodes The royal cinclodes, a large-billed ovenbird, is native to the high-altitude, semihumid Polylepis or Polylepis - Gynoxys woodlands of the Bolivian and Peruvian Andes, where it occupies a narrow range of distribution at elevations between 11,483 and 12,092 ft (3,500 and 4,600 m). Preferring dense woodlands with more closed canopies, the royal cinclodes is a ground-feeding insectivore that probes the mossy forest undergrowth for food. The species has a highly restricted and severely fragmented range (approximately 1,042 mi\2\ (2,700 km\2\)), and is found only in the Peruvian Administrative Regions of Apurimac, Cusco, Junin, and Puno, and in the Bolivian Department of La Paz. The known population of the royal cinclodes is estimated to be 239-259 individuals. We have carefully assessed the best available scientific and commercial information regarding the past, present, and potential future threats faced by the royal cinclodes and have concluded that there are three primary factors impacting the continued existence of the royal cinclodes: (1) Habitat destruction, fragmentation, and degradation; (2) limited size and isolation of remaining populations; and (3) inadequate regulatory mechanisms. Human activities that degrade, alter, and destroy habitat are ongoing throughout the royal cinclodes' range. Widespread deforestation and the conversion of forests for grazing, agriculture, and human settlement have led to the fragmentation of habitat throughout the range of the royal cinclodes (Factor A). Researchers estimate that only 1 percent of the dense Polylepis woodlands preferred by the species remain extant. Limited by the availability of suitable habitat, the species occurs today only in some of these fragmented and disjunct locations. Royal cinclodes habitat is particularly vulnerable to the drying effects accompanied by diminished forest cover. Royal cinclodes habitat continues to be altered by human activities, which result in the continued degradation, conversion, and destruction of habitat and reduction of the quantity, quality, distribution, and regeneration of remaining patches. Habitat loss was a factor in this species' historical decline, resulting in extirpation of the species from its type locality and, possibly, the loss of a viable population in the entire Region of Puno, Peru (see Historical Range and Distribution). Thespecies' population is considered to be declining in association with the reduction in habitat (Factors A and E). The royal cinclodes population is small, rendering the species particularly vulnerable to the threat of adverse natural events (e.g., genetic, demographic, or environmental) and human activities (e.g., deforestation and habitat alteration) that destroy individuals and their habitat. Human activities that continue to curtail the species' habitat throughout its range exacerbate the genetic and demographic risks associated with small population sizes (Factor E). The population has declined 30-49 percent in the past 10 years (see Population Estimates), and is predicted to continue declining commensurate with ongoing habitat loss (Factor A). Current research indicates that narrow endemics, such as the royal cinclodes, are especially susceptible to climate fluctuations because of the synergistic effect these fluctuations have on declining populations that are also experiencing range reductions due to human activities (Factor A). Despite the species' ``critically endangered'' status in Peru and its occurrence within several protected areas in Peru and Bolivia (Factor D), the species' habitat continues to be destroyed and degraded as a result of human activities (Factor A), which corresponds with population declines (Factor E). Therefore, regulatory mechanisms are either inadequate or ineffective at mitigating the existing threats to the royal cinclodes and its habitat (Factor D). Section 3 of the Act defines an ``endangered species'' as ``any species which is in danger of extinction throughout all or a significant portion of its range'' and a ``threatened species'' as ``any species which is likely to become an endangered species within the foreseeable future throughout all or a significant portion of its range.'' Based on the immediate and ongoing threats to the royal cinclodes throughout its entire range, as described above, we determine that the royal cinclodes is in danger of extinction throughout all of its range. Therefore, on the basis of the best available scientific and commercial information, we are proposing to list the royal cinclodes as an endangered species throughout all of its range. VI. White-browed tit-spinetail (Leptasthenura xenothorax) Species Description The white-browed tit-spinetail, or ``tijeral cejiblanco,'' is a small dark ovenbird in the Furnaridaii family that is native to high- altitude woodlands of the Peruvian Andes (BLI 2000, p. 347; Chapman 1921, pp. 8-9; del Hoyo et al. 2003, pp. 266-267; Fjeldsa and Krabbe 1990, p. 348; Parker and O'Neill 1980, p. 169). The sexes are similar, with individuals approximately 7 in (18 cm) in length. The species is characterized by its bright rufous crown and prominent white supercilium (eyebrow) (del Hoyo et al. 2003, p. 267; Lloyd 2009, p. 2), which gives the species its name. The species is highly vocal, ``often singing while acrobatically foraging from the outermost branches of Polylepis trees'' (Lloyd 2009, p. 2). Taxonomy The white-browed tit-spinetail was first described by Chapman in 1921 (del Hoyo et al. 2003, p. 267). The species has been synonymized with the nominate subspecies of the rusty-crowned tit-spinetail (Leptasthenura pileata pileata) by Vaurie (1980, p. 66), but examination of additional specimens in combination with field observations strongly suggests that L. xenothorax is a valid species (Collar et al. 1992, p. 596; Fjeldsa and Krabbe 1990, p. 348; Parker and O'Neill 1980, p. 169). Therefore, we accept the species as Leptasthenura xenothorax, which also follows the Integrated Taxonomic Information System (ITIS 2009, p. 1). Habitat and Life History The white-browed tit-spinetail is restricted to high-elevation, semihumid Polylepis and Polylepis-Gynoxys woodlands, where the species is found between 12,139 and 14,928 ft (3,700 and 4,550 m) above sea level (BLI 2000, p. 347; Collar et al. 1992, p. 595; del Hoyo et al. 2003, p. 267; Fjeldsa and Krabbe 1990, p. 348; Lloyd 2009, pp. 5-6). The characteristics of Polylepis habitat were described above as part of the Habitat and Life History of the ash-breasted tit-tyrant (Aucca and Ramsay 2005, p. 1; Chutas et al. 2008, p. 3; De la Via 2004, p. 10; IPNI 2009, p. 1; Kessler 1998, p. 1; Purcell et al. 2004, p. 455). The white-browed tit-spinetail prefers areas of primary forest that have a high density of tall, large trees and dense Polylepis [[Page 643]] stands and vegetation cover (Lloyd 2008a, as cited in Lloyd 2009, p. 6). Dense stands of Polylepis woodlands are characterized by moss-laden vegetation and a shaded understory, and provide for a rich diversity of insects, making these areas good feeding grounds for insectivorous birds (De la Via 2004, p. 10), such as the white-browed tit-spinetail (BLI 2009d, p. 2). According to Engblom et al. (2002, pp. 57-58), the species has been recorded in patches of woodland as small as 0.6 ac (0.25 ha) in Cordillera Vilcabamba. Based on these observations, Engblom et al. (2002, p. 58) suggest that the species is able to persist in very small forest fragments, especially if a number of these patches are in close proximity. The lower elevation of this species' range changes to a mixed Polylepis-Escallonia (no common name) woodland, and the white-browed tit-spinetail has been observed there on occasion, such as during a snowstorm (Collar et al. 1992, p. 595; del Hoyo et al. 2003, p. 267; Fjeldsa and Krabbe 1990, p. 348). There is limited information the ecology and breeding behavior of the white-browed tit-spinetail. Lloyd (2006, as cited in Lloyd 2009, p. 8) reports that the species breeds in October in Cordillera Vilcanota in southern Peru. In the same area, one adult was seen attending a nesting hole in a Polylepis tree in November 1997 (del Hoyo et al. 2003, p. 267; C. Bushell in litt. (1999), as cited in BLI 2009d, p. 2). Only one nest of the white-browed tit-spinetail has ever been described. According to Lloyd (2006, as cited in Lloyd 2009, p. 8), the nest was located within a natural cavity of a Polylepis racemosa tree's main trunk, approximately 7 ft (2 m) above the ground. To construct their nest, the white-browed tit-spinetail pair uses moss, lichen, and bark fibers they stripped from Polylepis tree trunks, large branches and large boulders while foraging. The nest was cup-shaped and contained two pale-colored eggs (Lloyd 2006, as cited in Lloyd 2009, p. 8). The white-browed tit-spinetail is insectivorous, with a diet consisting primarily of arthropods (del Hoyo et al. 2003, p. 267; Lloyd 2009, p. 7). The species forages in pairs or small family groups of three to five, and often in mixed-species flocks, gleaning insects from bark crevices, moss, and lichens on twigs, branches, and trunks (BLI 2009d, pp. 2-3; Engblom et al. 2002, pp. 57-58; Parker and O'Neill 1980, p. 169). The white-browed tit-spinetail is highly arboreal, typically foraging acrobatically from the outer branches of Polylepis trees while hanging upside-down (del Hoyo et al. 2003, p. 267; Lloyd 2008b, as cited in Lloyd 2009, p. 7). Historical Range and Distribution In our 2008 Annual Notice of Findings on Resubmitted Petitions for Foreign Species (73 FR 44062; July 29, 2008), we stated that historically, the white-browed tit-spinetail may have occupied the Polylepis forests of the high-Andes of Peru and Bolivia. We included both countries in the historical range of the species because the species' primary habitat, the Polylepis forest, was historically large and contiguous throughout the high-Andes of both Peru and Bolivia (Fjeldsa 2002a, p. 115). However, based on further research, we have determined that historically, the species was only known from two Regions in south-central Peru, Cusco and Apurimac (Collar et al. 1992, p. 594; del Hoyo et al. 2003, p. 267), and not in Bolivia. The white-browed tit-spinetail may once have been distributed throughout south-central Peru, in previously contiguous Polylepis forests above 9,843 ft (3,000 m) (BLI 2000, p. 347; BLI 2009d, pp. 1-2; Fjeldsa 2002a, pp. 111-112, 115; Herzog et al. 2002, p. 94; Kessler 2002, pp. 97-101). However, Polylepis woodlands are now restricted to elevations of 11,483 to 16,404 ft (3,500 to 5,000 m) (Fjeldsa 1992, p. 10). As discussed above for the Historical Range and Distribution of the ash-breasted tit-tyrant, researchers consider human activity to be the primary cause for historical habitat decline and resultant decrease in species richness (Fjeldsa and Kessler 1996, Kessler 1995a, b, and L[aelig]gaard 1992, as cited in Fjeldsa 2002a, p. 112; Fjeldsa 2002a, p. 116; Herzog et al. 2002, p. 94; Kessler 2002, pp. 97-101; Kessler and Herzog 1998, pp. 50-51). It is estimated that only 2-3 percent of the original forest cover still remains in Peru (Fjeldsa 2002a, pp. 111, 113). Less than 1 percent of the remaining woodlands occur in humid areas, where denser stands are found (Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113), and which are preferred by the white-browed tit-spinetail (BLI 2009d, p. 2; Lloyd 2008a, as cited in Lloyd 2009, p. 6). Current Range and Distribution Today, the white-browed tit-spinetail is restricted to high- elevation, semihumid patches of Polylepis and Polylepis-Gynoxys woodlands in the Andes mountains of south-central Peru, where the species occurs between 12,139-14,928 ft (3,700-4,550 m) (BLI 2000, p. 347; Collar et al. 1992, p. 595; del Hoyo et al. 2003, p. 267; Fjeldsa and Krabbe 1990, p. 348; InfoNatura 2007, p. 1; Lloyd 2009, pp. 1, 5- 6). The species has a highly restricted and severely fragmented range, and is currently known from only a small number of sites: The Runtacocha highlands (in Apurimac Region) and the Nevado Sacsarayoc massif, Cordillera Vilcabamba (Chapman 1921, p. 8), and Cordillera Vilcanota (in the Cusco Region) (BLI 2000, p. 347; BLI 2009d, p. 2; Lloyd 2009, p. 5). The estimated range of the species is approximately 965 mi\2\ (2,500 km\2\) (BLI 2000, p. 347; BLI 2009d, pp. 1, 5). Population Estimates Population information is presented first on the local level and then in terms of a global population estimate. Local population estimates: Between 1987 and 1989, populations of 35-70 individuals were estimated to occur at 3 sites in Cusco; since then, declines in the populations at some of these sites have been observed (Fjeldsa and Kessler 1996, as cited in BLI 2000, p. 347). At Abra Malaga (Cusco Region), it is estimated that there are approximately 30-50 birds (del Hoyo et al. 2003, p. 267; Engblom et al. 2002, p. 58). In the Runtacocha highlands (Apurimac Region), the population density of the white-browed tit-spinetail is very low (Fjeldsa and Kessler 1996, as cited in BLI 2000, p. 347). Chutas (2007, p. 8) reported an estimated 305 birds located within 3 disjunct Polylepis forest patches in Peru. This included 205 birds and 36 birds in Cordilleras Vilcanota and Vilcabamba, respectively (Cusco), and 64 birds in ``Cordillera del Apurimac'' (Runtacocha highlands of Apurimac) (Chutas 2007, p. 8). Density estimates derived from surveys conducted at 3 sites in Cordillera Vilcanota range from 25.3 ( 15.1) individuals per km\2\, to 9.6 ( 21.7) individuals per km\2\, and the species may occur at even higher densities in other areas of Polylepis forests (Lloyd 2008c, as cited in Lloyd 2009, p. 9). According to Lloyd (2008c, as cited in Lloyd 2009, p. 9), this quantitative data from Cordillera Vilcanota shows that the white-browed tit-spinetail is ``one of the most abundant Polylepis specialists in southern Peru.'' Global population estimate: BirdLife International categorizes the white-browed tit-spinetail as having a population size between 500 and 1,500 individuals (BLI 2009d, pp. 1, 5). The category was determined from the population estimates reported by Engblom et al. 2002 (p. 58), who estimated ``the known population to be around 500 individuals with a possible total population of 1,500 individuals.'' [[Page 644]] In 2002, Fjeldsa (2002b, p. 9) estimated a total population size of between 250 and 1,000 pairs of birds, which coincides with the BirdLife International category of 500-1,500 individuals. The species has experienced a population decline of between 10 and 19 percent in the past 10 years, and this rate of decline is predicted to continue (BLI 2009d, p. 5). The population is considered to be declining in close association with continued habitat loss and degradation (see Factors A and E) (BLI 2009d, p. 6). Conservation Status The white-browed tit-spinetail is considered ``endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276854). The IUCN considers the white-browed tit-spinetail to be ``Endangered'' due to its very small and severely fragmented range and population, which continue to decline with ongoing habitat loss and a lack of habitat regeneration (BLI 2009d, p. 1). The white-browed tit- spinetail occurs within the Peruvian protected area of Santuario Historico Machu Picchu in Cusco (BLI 2009c, pp. 1, 3; BLI 2009d, p. 6; del Hoyo et al. 2003, p. 267). Summary of Factors Affecting the White-browed Tit-Spinetail A. The Present or Threatened Destruction, Modification, or Curtailment of the Species' Habitat or Range The white-browed tit-spinetail is restricted to high-elevation, semihumid Polylepis and Polylepis-Gynoxys woodlands, where it is found between 12,139 and 14,928 ft (3,700 and 4,550 m) above sea level (BLI 2000, p. 347; Collar et al. 1992, p. 595; del Hoyo et al. 2003, p. 267; Fjeldsa and Krabbe 1990, p. 348; Lloyd 2009, pp. 1, 5-6). High-Andean Polylepis habitat is characterized as a threatened woodland ecosystem on national, regional, and global levels (Purcell et al. 2004, p. 457; Renison et al. 2005, as cited in Lloyd 2009, p. 10), with several Polylepis species within the white-browed tit-spinetail's range considered to be ``Vulnerable,'' according to the IUCN (WCMC 1998a, p. 1; WCMC 1998b, p. 1). As described more fully for the ash-breasted tit- tyrant (Factor A), Polylepis woodlands have been much reduced from historical estimates, with an estimated remaining area of 386 mi\2\ (1,000 km\2\) in Peru (Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113). The majority of these remaining forests are much dispersed, and less than 1 percent is located in the humid parts of the highlands, where denser stands occur (Fjeldsa and Kessler 1996, as cited in Fjeldsa 2002a, p. 113). The white-browed tit-spinetail prefers areas of dense Polylepis primary forest with understory vegetation that provides optimal foraging habitat (BLI 2009d, p. 2; De la Via 2004, p. 10; Lloyd 2008a, as cited in Lloyd 2009, p. 6) (see Habitat and Life History). In the Cordillera de Vilcanota (Cusco, Peru), where a large portion of the known white-browed tit-spinetail population occurs (205 birds were observed there, out of 305 total birds observed in 3 study sites in Peru) (Chutas 2007, p. 8), Polylepis woodland habitat is highly fragmented and degraded. As described more fully for the ash-breasted tit-tyrant (Factor A), recent research indicated that: (1) Four forest patches in the Cordillera de Vilcanota disappeared completely in the last half a century, and the remaining Polylepis remnants are small (with a mean patch size of 7.4 ac (3 ha) (Jameson and Ramsay 2007, p. 42) and commonly separated from the larger patches by distances of 98-4,921 ft (30-1,500 m) (Lloyd and Marsden in press, as cited in Lloyd 2008, p. 532); (2) Ten percent of the remaining forest patches showed a decline in forest density (Jameson and Ramsay 2007, p. 42); and (3) There were no indications of forest regeneration within the study area. These findings have consequences for the white-browed tit-spinetail given the species' ecological requirements. As Polylepis woodlands decline in number, the distances between patches increase. According to Engblom et al. (2002, pp. 57-58), the species has been recorded in patches of woodland as small as 0.6 ac (0.25 ha) in Cordillera Vilcabamba, but the species' persistence in small patches appears to be dependent on the patches being in close proximity to each other. Habitat degradation impacts the white-browed tit-spinetail, given its preference for dense Polylepis woodlands, where optimal foraging habitat is found (BLI 2009d, p. 2; De la Via 2004, p. 10; Lloyd 2008a, as cited in Lloyd 2009, p. 6). The lack of Polylepis forest regeneration in the area over nearly 50 years underscores the ramifications of continued burning and clearing to maintain pastures and farmland that are prevalent throughout the white-browed tit- spinetail's range (BLI 2009a, p. 1; Engblom et al. 2002, p. 56; Fjeldsa 2002a, pp. 112, 120; Fjeldsa 2002b, p. 8; Purcell et al. 2004, p. 458; WCMC 1998a, p. 1; WCMC 1998b, p. 1). Habitat loss, conversion, and degradation throughout the white- browed tit-spinetail's range are attributed to human activities (a full description of which is provided above as part of the Factor A analysis for the ash-breasted tit-tyrant). Ongoing activities include: (1) Clearcutting and uncontrolled burning for agriculture and pastureland for domesticated animals, all of which contributes to soil erosion, and habitat degradation, which prevent forest regeneration and restrict Polylepis woodlands to localized and highly fragmented landscapes (BLI 2009a, p. 2; BLI 2009b, p. 1; BLI 2009c, p. 3; BLI 2009d, p. 3; Engblom et al. 2002, p. 56; Fjeldsa 2002a, pp. 112, 120; Fjeldsa 2002b, p. 8; Jameson and Ramsay 2007, p. 42; Purcell et al. 2004, p. 458; Renison et al. 2006, as cited in Lloyd 2009, p. 11; WCMC 1998a, p. 1; WCMC 1998b, p. 1); (2) Extractive activities, such as harvest for timber, firewood, and charcoal, for use on local- and commercial-scales as fuel, construction, fencing and tool-making (Aucca and Ramsay 2005, p. 287; BLI 2009a, p. 2; BLI 2009b, p. 1; BLI 2009d, p. 3; Engblom 2000, pp. 1- 2; Engblom et al. 2002, p. 56; Fjeldsa and Kessler 1996, as cited in BLI 2009d, p. 3; Purcell et al. 2004, pp. 458-459; WCMC 1998a, p. 1); and (3) Unpredictable climate fluctuations that exacerbate the effects of habitat fragmentation (Jetz et al. 2007, pp. 1211, 1213; Mora et al. 2007, p. 1027). These habitat-altering activities are ongoing throughout the range of the white-browed tit-spinetail, including in Apurimac (BLI 2009b, p. 1) and Cusco (BLI 2009a, pp. 1-2; BLI 2009c, pp. 1-3) of south-central Peru and within the one protected area in which the species occurs, Santuario Historico Machu Picchu (BLI 2009c, p. 3). Polylepis conservation programs have been under way in Peru since 2004, including in Cordilleras Vilcanota and Vilcabamba and highlands of the Apurimac Region, where white-browed tit-spinetail also occurs (Aucca and Ramsey 2005, p. 287; Chutas 2007, p. 8; ECOAN n.d., p. 1; Lloyd 2009, p. 10). These community-based programs, which are more fully described above as part of the Factor A analysis for the ash- breasted tit-tyrant, confront the primary causes of Polylepis deforestation: Burning, grazing, and wood-cutting. One such program, called the ``Vilcanota Project,'' is under way at three locations in the Cordillera de Vilcanota (Abra Malaga, Hulloc, and Cancha-Cancha) (Aucca and Ramsay 2005, p. 287; ECOAN n.d., p. 1; Lloyd 2009, p. 10). Since local populations rely on Polylepis wood for firewood and [[Page 645]] charcoal production (Aucca and Ramsay 2005, p. 287; Engblom et al. 2002, p. 56), the Vilcanota Project works to deliver non- Polylepis firewood to families for cooking, as well as supply them with fuel- efficient cooking stoves (ECOAN n.d., p. 1). A short-term aim of these projects is to restore balance to local sustainable resource use (Aucca and Ramsay 2005, p. 288; ECOAN n.d., p. 1). However, at Abra Malaga (one of the Vilcanota Project's sites), Polylepis woodlands continue to be impacted by extraction for firewood and burning for agriculture and pastureland (BLI 2009a, pp. 1-2). In addition, commercial-scale activities, such as logging and fuel wood collection, which are ongoing throughout this species' range, alter otherwise sustainable resource use practices (Aucca and Ramsay 2005, p. 287; Engblom 2000, p. 2; Engblom et al. 2002, p. 56; MacLennan 2009, p. 2; Purcell et al. 2004, pp. 458-459; WCMC 1998a, p. 1). Habitat destruction caused by burning and grazing, which have prevented regeneration of habitat, is a factor in the historical decline of Polylepis -dependent bird species (Fjeldsa 2002a, p. 116). The white-browed tit-spinetail's population size is considered to be declining in close association with the continued habitat loss and degradation of Polylepis woodlands (BLI 2009d, p. 6). The species may once have been distributed throughout south-central Peru, in once contiguous Polylepis forests (BLI 2000, p. 347; BLI 2009d, pp. 1-2; Fjeldsa 2002a, pp. 111-112, 115; Herzog et al. 2002, p. 94; Kessler 2002, pp. 97-101). Today, the species has a highly restricted and severely fragmented range, and is currently known from only a small number of sites in the Regions of Apurimac and Cusco in south-central Peru (BLI 2000, p. 347; BLI 2009d, pp. 1-2; Lloyd 2009, p. 5). White-browed tit-spinetails are also impacted by unpredictable climate fluctuations, which are more fully described under the Factor A analysis of the ash-breasted tit-tyrant and are summarized here. Peru is subject to unpredictable climate fluctuations that exacerbate the effects of habitat fragmentation, such as those that are related to the El Ni[ntilde]o Southern Oscillation (ENSO). Changes in weather patterns, such as ENSO cycles (El Ni[ntilde]o and La Ni[ntilde]a events), tend to increase precipitation in normally dry areas, and decrease precipitation in normally wet areas (Holmgren et al. 2001, p. 89; TAO Project n.d., p. 1), exacerbating the effects of habitat fragmentation on the decline of a species (England 2000, p. 86; Holmgren et al. 2001, p. 89; Jetz et al. 2007, pp. 1211, 1213; Mora et al. 2007, p. 1027; Parmesan and Mathews 2005, p. 334; Plumart 2007, pp. 1-2; Timmermann 1999, p. 694), especially for narrow endemics (Jetz et al. 2007, p. 1213) such as the white-browed tit-spinetail (see also Factor E). ENSO cycles strongly influence the growth of Polylepis species (Christie et al. 2008, p. 1) by altering Polylepis species' age structure and mortality, especially where woodlands have undergone disturbance, such as fire and grazing (Villalba and Veblen 1997, pp. 121-123; Villalba and Veblen 1998, pp. 2624, 2637). These cycles may have already accelerated the fire cycle (Block and Richter 2007, p. 1; Power et al. 2007, pp. 897-898), which is a key factor preventing Polylepis regeneration (Fjeldsa 2002a, p. 112, 120; Fjeldsa 2002b, p. 8) because Polylepis species recover poorly following a fire (Cierjacks et al. 2007, p. 176). ENSO cycles are ongoing, having occurred several times within the last decade (NWS 2009, p. 2), and evidence suggests that ENSO cycles have already increased in periodicity and severity (Richter 2005, pp. 24-25; Timmermann 1999, p. 694), which will exacerbate the negative impacts of habitat destruction on a species. It is predicted that, by 2050, another 1 percent of the white-browed tit- spinetail's current remaining range is likely to be unsuitable for this species due to climate change; and, by 2100, it is predicted that about 43 percent of the species' range is likely to be lost as a direct result of global climate change (Jetz et al. 2007, Supplementary Table 2, p. 89). Summary of Factor A Polylepis habitat throughout the range of the white-browed tit- spinetail has been and continues to be altered and destroyed as a result of human activities, including clearcutting and burning for agriculture and grazing lands and extractive activities, including harvest for timber, firewood, and charcoal. It is estimated that only 1 percent of the dense Polylepis woodlands preferred by the species remain, and the remaining woodlands are highly fragmented and degraded. Observations suggest that the white-browed tit-spinetail is able to persist in very small forest fragments (e.g., areas as small as 0.6 ac (0.25 ha) in Cordillera Vilcabamba); however, this depends on whether or not a number of patches are in close proximity to one another. Since the remaining Polylepis woodlands are and continue to be severely fragmented, the distance between some of the small woodland patches may be too large for the species to be able to persist. Today, the species is known from only a small number of sites at four locations: The Runtacocha highlands (in Apurimac Region), and the Nevado Sacsarayoc massif, Cordillera Vilcabamba, and Cordillera Vilcanota (in Cusco Region). Historical decline in habitat availability is attributed to the same human activities that are causing habitat loss today. Ongoing and accelerated habitat destruction of the remaining Polylepis woodlands in Peru continues to reduce the quantity, quality, distribution, and potential regeneration of Polylepis forests. Human activities that degrade, alter, and destroy habitat are ongoing throughout the species' range, including within protected areas (see also Factor D). Experts consider the species' population decline to be commensurate with the declining habitat (Factor E). Current research indicates that climate fluctuations exacerbate the effects of habitat loss to species, especially for narrow endemics such as the white- browed tit-spinetail that are already undergoing range reduction due to human activities. Climate models predict that this species' habitat will continue to decline. Therefore, we find that destruction and modification of habitat are threats to the continued existence of the white-browed tit-spinetail throughout its range. B. Overutilization for Commercial, Recreational, Scientific, or Educational Purposes We are not aware of any information currently available that indicates that overutilization of the species for commercial, recreational, scientific, or educational purposes has occurred or is occurring at this time. As a result, we are not considering overutilization to be a threat to the continued existence of the white- browed tit-spinetail. C. Disease or Predation We are not aware of any scientific or commercial information that indicates that disease or predation poses a threat to the species. As a result, we are not considering disease or predation to be a threat to the continued existence of the white-browed tit-spinetail. D. Inadequacy of Existing Regulatory Mechanisms The white-browed tit-spinetail is considered ``endangered'' by the Peruvian Government under Supreme Decree No. 034-2004-AG (2004, p. 276854). This Decree prohibits hunting, take, transport, and trade of protected species, except as permitted by regulation. As hunting, taking, or trade do not currently threaten the white-browed tit- spinetail, this regulation does [[Page 646]] not mitigate any current threats to the species. Peru has several categories of national habitat protection, which were described above as part of Factor D for the ash-breasted tit- tyrant (IUCN 1994, p. 2; Rodriguez and Young 2000, p. 330). Protected areas have been established through regulation at one site occupied by the white-browed tit-spinetail in Peru: the Santuario Historico Machu Picchu (Cusco, Peru); (BLI 2009c, pp. 1, 3; BLI 2009d, p. 6). Resources within Santuario Historico Machu Picchu are supposed to be managed for conservation (Rodriguez and Young 2000, p. 330). However, habitat destruction and alteration, including burning, cutting, and grazing are ongoing within the sanctuary, preventing regeneration of the woodlands (BLI 2009c, p. 3; Engblom et al. 2002, p. 58). Therefore, the occurrence of the white-browed tit-spinetail within protected areas in Peru does not protect the species, nor does it mitigate the threats to the species from ongoing habitat loss (Factor A) and concomitant population decline (Factor E). Summary of Factor D Peru has enacted various laws and regulatory mechanisms to protect and manage wildlife and their habitats. The white-browed tit-spinetail is ``endangered'' under Peruvian law and occurs within one protected area in Peru. As discussed under Factor A, the white-browed tit- spinetail prefers dense Polylepis woodlands, which have been reduced by an estimated 98 percent in Peru. The Polylepis habitat that does remain is highly fragmented and degraded. Habitat throughout the species' range has been and continues to be altered as a result of human activities, including clearcutting and burning for agriculture and grazing lands; and extractive activities such as timber harvest, firewood collection, and charcoal production. These activities are ongoing within protected areas despite the species' endangered status, indicating that the laws governing wildlife and habitat protection in Peru are either inadequate or inadequately enforced to protect the species or to mitigate ongoing habitat loss (Factor A) and population declines (Factor E). Therefore, we find that the existing regulatory mechanisms are inadequate to mitigate the current threats to the continued existence of the white-browed tit-spinetail throughout its range. E. Other Natural or Manmade Factors Affecting the Continued Existence of the Species An additional factor that affects the continued existence of the white-browed tit-spinetail is the species' small population size. As discussed above (see Population Estimates), BirdLife International has placed the white-browed tit-spinetail in the population category of between 500 and 1,500 individuals (BLI 2009d, pp. 1, 5), and characterizes the species as having a ``very small population'' size (BLI 2000, p. 347; BLI 2009d, p. 1). Small population size renders species vulnerable to genetic risks that can have individual or population-level genetic consequences, such as inbreeding depression, loss of genetic variation, and accumulation of new mutations, and may affect the species' viability by increasing its susceptibility to demographic shifts or environmental fluctuations, as explained in more detail above in the Factor E analysis for the ash- breasted tit-tyrant (Charlesworth and Charlesworth 1987, p. 238; Pimm et al. 1988, pp. 757, 773-775; Shaffer 1981, p. 131). Small population size leads to a higher risk of extinction and, once a population is reduced below a certain number of individuals, it tends to rapidly decline towards extinction (Frankham 1996, p. 1507; Franklin 1980, pp. 147-148; Gilpin and Soule 1986, p. 25; Holsinger 2000, pp. 64-65; Purvis et al. 2000, p. 1949; Reed and Frankham 2003, pp. 233-234; Soule 1987, p. 181). Complications arising from the species' small population size are exacerbated by the species' fragmented distribution. The white-browed tit-spinetail is currently confined to high-elevation, semihumid patches of forest in the Andes of Peru, and its population has declined at a rate between 10 and 19 percent in the past 10 years, in close association with the continued loss and degradation of the Polylepis forest (BLI 2009d, pp. 5-6) (Factor A). Fjeldsa and Kessler (1996, as cited in Fjeldsa 2002a, p. 113) describe the remaining Polylepis woodlands as highly fragmented and degraded, and estimate that only 1 percent of the dense woodlands preferred by the species remain (BLI 2009d, p. 2; De la Via 2004, p. 10; Lloyd 2008a, as cited in Lloyd 2009, p. 6) (see Habitat and Life History). As a result, experts say that the species' current range is highly restricted and severely fragmented (BLI 2000, p. 347; BLI 2009d, p. 1; Collar et al. 1992, p. 595; del Hoyo et al. 2003, p. 267; Fjeldsa and Krabbe 1990, p. 348; InfoNatura 2007, p. 1; Lloyd 2009, p. 5). The species' small population size, combined with its highly restricted and severely fragmented range, increases the species' vulnerability to adverse natural events and manmade activities that destroy individuals and their habitat (Holsinger 2000, pp. 64-65; Primack 1998, pp. 279-308; Young and Clarke 2000, pp. 361-366). Summary of Factor E The white-browed tit-spinetail has a small population size that renders it vulnerable to genetic risks that negatively impact the species' viability. The species has a severely restricted range and occurs in highly fragmented habitat that continues to undergo degradation and curtailment due to human activities (Factor A). The restricted and fragmented range, as well as the small population size, increases the species' vulnerability to extinction through demographic or environmental fluctuations. Based on its small population size and fragmented distribution, we have determined that the white-browed tit- spinetail is particularly vulnerable to the threat of adverse natural events (e.g., genetic, demographic, or environmental) and human activities (e.g., deforestation and habitat alteration) that destroy individuals and their habitat. The genetic and demographic risks associated with small population sizes are exacerbated by ongoing human activities that continue to curtail the species' habitat throughout its range. The species' population has declined and is predicted to continue declining commensurate with ongoing habitat loss (Factor A). Therefore, we find that the species' small population size, in concert with its fragmented distribution and its heightened vulnerability to adverse natural events and manmade activities, are threats to the continued existence of the white-browed tit-spinetail throughout its range. Status Determination for the White-Browed Tit-Spinetail The white-browed tit-spinetail, a small dark ovenbird, is restricted to high-altitude woodlands of the Peruvian Andes. Preferring dense, semihumid Polylepis and Polylepis-Gynoxys woodlands, the ash- breasted tit-tyrant occupies a narrow range of distribution at elevations between 12,139 and 14,928 ft (3,700 and 4,550 m) above sea level. The species has a highly restricted and severely fragmented range (approximately 965 mi\2\ (2,500 km\2\)), and is currently known from only a small number of sites in the Apurimac and Cusco Regions, in south-central Peru. The known population of the [[Page 647]] white-browed tit-spinetail is estimated to be approximately 500 to 1,500 individuals. We have carefully assessed the best available scientific and commercial information regarding the past, present, and potential future threats faced by the white-browed tit-spinetail. There are three primary factors impacting the continued existence of the white-browed tit-spinetail: (1) Habitat destruction, fragmentation, and degradation; (2) limited size and isolation of remaining populations; and (3) inadequate regulatory mechanisms. Human activities that degrade, alter, and destroy habitat are ongoing throughout the white-browed tit-spinetail. Widespread deforestation and the conversion of forests for grazing and agriculture have led to the fragmentation of habitat throughout the range of the white-browed tit-spinetail (Factor A). Researchers estimate that only 1 percent of the dense Polylepis woodlands preferred by the species remain. Limited by the availability of suitable habitat, the species occurs today only in a few fragmented and disjunct locations. White-browed tit-spinetail habitat continues to be altered by human activities, which result in the continued degradation, conversion, and destruction of habitat and reduction of the quantity, quality, distribution, and regeneration of remaining forest patches. Habitat loss was a factor in this species' historical decline (see Historical Range and Distribution), and the species is considered to be declining today in association with the continued reduction in habitat (Factors A and E). The species' severely restricted range, combined with its small population size, renders it particularly vulnerable to the threat of adverse natural (e.g., genetic, demographic, or environmental) and manmade (e.g., deforestation, habitat alteration, wildfire) events that destroy individuals and their habitat. Human activities that continue to curtail the species' habitat throughout its range exacerbate the genetic and demographic risks associated with small population sizes (Factor E). The species has experienced a population decline of between 10 and 19 percent in the past 10 years (see Population Estimates), and is predicted to continue declining commensurate with ongoing habitat loss and degradation. Current research indicates that narrow endemics, such as the white-browed tit-spinetail, are especially susceptible to climate fluctuations because of the synergistic effect these fluctuations have on declining populations that are also experiencing range reductions due to human activities (Factor A). Despite the species' endangered status in Peru and its occurrence within one protected area, human activities that degrade, alter, and destroy habitat are ongoing throughout the white-browed tit-spinetail's range, including within protected areas. Therefore, regulatory mechanisms are either inadequate or ineffective at curbing the threats to the white-browed tit-spinetail of habitat loss (Factor A) and corresponding population decline (Factor E). Section 3 of the Act defines an ``endangered species'' as ``any species which is in danger of extinction throughout all or a significant portion of its range'' and a ``threatened species'' as ``any species which is likely to become an endangered species within the foreseeable future throughout all or a significant portion of its range.'' Based on the immediate and ongoing threats to the white-browed tit-spinetail throughout its entire range, as described above, we determine that the white-browed tit-spinetail is in danger of extinction throughout all of its range. Therefore, on the basis of the best available scientific and commercial information, we are proposing to list the white-browed tit-spinetail as an endangered species throughout all of its range. Available Conservation Measures Conservation measures provided to species listed as endangered or threatened under the Act include recognition, requirements for Federal protection, and prohibitions against certain practices. Recognition through listing results in public awareness, and encourages and results in conservation actions by Federal and State governments, private agencies and interest groups, and individuals. Section 7(a) of the Act, as amended, and as implemented by regulations at 50 CFR part 402, requires Federal agencies to evaluate their actions within the United States or on the high seas with respect to any species that is proposed or listed as endangered or threatened, and with respect to its critical habitat, if any has been proposed or designated. However, given that the ash-breasted tit-tyrant, Junin grebe, Junin rail, Peruvian plantcutter, the royal cinclodes, and the white-browed tit-spinetail are not native to the United States, we are not proposing critical habitat for these species under section 4 of the Act. Section 8(a) of the Act authorizes the provision of limited financial assistance for the development and management of programs that the Secretary of the Interior determines to be necessary or useful for the conservation of endangered and threatened species in foreign countries. Sections 8(b) and 8(c) of the Act authorize the Secretary to encourage conservation programs for foreign endangered and threatened species and to provide assistance for such programs in the form of personnel and the training of personnel. The Act and its implementing regulations set forth a series of general prohibitions and exceptions that apply to all endangered and threatened wildlife. As such, these prohibitions would be applicable to the ash-breasted tit-tyrant, Junin grebe, Junin rail, Peruvian plantcutter, the royal cinclodes, and the white-browed tit-spinetail. These prohibitions, under 50 CFR 17.21, make it illegal for any person subject to the jurisdiction of the United States to ``take'' (take includes to harass, harm, pursue, hunt, shoot, wound, kill, trap, capture, or collect, or to attempt to engage in any such conduct) any endangered wildlife species within the United States or upon the high seas; or to import or export; deliver, receive, carry, transport, or ship in interstate or foreign commerce in the course of commercial activity; or to sell or offer for sale in interstate or foreign commerce any endangered wildlife species. It is also illegal to possess, sell, deliver, carry, transport, or ship any such wildlife that has been taken in violation of the Act. Certain exceptions apply to agents of the Service and State conservation agencies. Permits may be issued to carry out otherwise prohibited activities involving endangered and threatened wildlife species under certain circumstances. Regulations governing permits are codified at 50 CFR 17.22 for endangered species. With regard to endangered wildlife, a permit may be issued for the following purposes: for scientific purposes, to enhance the propagation or survival of the species, and for incidental take in connection with otherwise lawful activities. Peer Review In accordance with our joint policy with National Marine Fisheries Service, ``Notice of Interagency Cooperative Policy for Peer Review in Endangered Species Act Activities,'' published in the Federal Register on July 1, 1994 (59 FR 34270), we will seek the expert opinions of at least three appropriate independent specialists regarding this proposed rule. The purpose of peer review is to ensure that our final determination is based on scientifically sound data, assumptions, and analyses. We will send copies of this proposed rule to the peer reviewers immediately [[Page 648]] following publication in the Federal Register. We will invite these peer reviewers to comment during the public comment period on our specific assumptions and conclusions regarding the proposal to list the ash-breasted tit-tyrant, Junin grebe, Junin rail, Peruvian plantcutter, royal cinclodes, and white-browed tit-spinetail. We will consider all comments and information we receive during the comment period on this proposed rule during our preparation of a final determination. Accordingly, our final decision may differ from this proposal. Public Hearings The Act provides for one or more public hearings on this proposal, if we receive any requests for hearings. We must receive your request for a public hearing within 45 days after the date of this Federal Register publication (see DATES). Such requests must be made in writing and be addressed to the Chief of the Branch of Listing at the address shown in the FOR FURTHER INFORMATION CONTACT section. We will schedule public hearings on this proposal, if any are requested, and announce the dates, times, and places of those hearings, as well as how to obtain reasonable accommodations, in the Federal Register at least 15 days before the first hearing. Required Determinations National Environmental Policy Act (NEPA) We have determined that environmental assessments and environmental impact statements, as defined under the authority of the National Environmental Policy Act of 1969 (42 U.S.C. 4321 et seq.), need not be prepared in connection with regulations adopted under section 4(a) of the Act. We published a notice outlining our reasons for this determination in the Federal Register on October 25, 1983 (48 FR 49244). Clarity of the Rule We are required by Executive Orders 12866 and 12988, and by the Presidential Memorandum of June 1, 1998, to write all rules in plain language. This means that each rule we publish must: (a) Be logically organized; (b) Use the active voice to address readers directly; (c) Use clear language rather than jargon; (d) Be divided into short sections and sentences; and (e) Use lists and tables wherever possible. If you feel that we have not met these requirements, send us comments by one of the methods listed in the ADDRESSES section. To better help us revise the rule, your comments should be as specific as possible. For example, you should tell us the numbers of the sections or paragraphs that are unclearly written, which sections or sentences are too long, the sections where you feel lists or tables would be useful, etc. References Cited A complete list of all references cited in this proposed rule is available on the Internet at http://www.regulations.gov or upon request from the Branch of Listing, Endangered Species Program, U.S. Fish and Wildlife Service (see FOR FURTHER INFORMATION CONTACT). Author(s) The primary authors of this proposed rule are Jesse D'Elia (of the Pacific Regional Office) and Patricia De Angelis, Patricia Ford, Monica Horton, and Marie Maltese (all of the Division of Scientific Authority), U.S. Fish and Wildlife Service. List of Subjects in 50 CFR Part 17 Endangered and threatened species, Exports, Imports, Reporting and recordkeeping requirements, Transportation. Proposed Regulation Promulgation Accordingly, we propose to amend part 17, subchapter B of chapter I, title 50 of the Code of Federal Regulations, as set forth below: PART 17--[AMENDED] 1. The authority citation for part 17 continues to read as follows: Authority: 16 U.S.C. 1361-1407; 16 U.S.C. 1531-1544; 16 U.S.C. 4201-4245; Pub. L. 99-625, 100 Stat. 3500; unless otherwise noted. 2. Amend Sec. 17.11(h) by adding new entries for ``Cinclodes, royal,'' ``Grebe, Junin,'' ``Plantcutter, Peruvian,'' ``Rail, Junin,'' ``Tit-spinetail, white-browed,'' and ``Tit-tyrant, ash-breasted'' in alphabetical order under BIRDS to the List of Endangered and Threatened Wildlife, as follows: Sec. 17.11 Endangered and threatened wildlife. * * * * * (h) * * * -------------------------------------------------------------------------------------------------------------------------------------------------------- Species Vertebrate ------------------------------------------------- population where Critical Historic range endangered or Status When listed habitat Special rules Common name Scientific name threatened -------------------------------------------------------------------------------------------------------------------------------------------------------- * * * * * * * -------------------------------------------------------------------------------------------------------------------------------------------------------- BIRDS -------------------------------------------------------------------------------------------------------------------------------------------------------- * * * * * * * -------------------------------------------------------------------------------------------------------------------------------------------------------- Cinclodes, royal Cinclodes Bolivia, Peru Entire E NA NA aricomae -------------------------------------------------------------------------------------------------------------------------------------------------------- * * * * * * * -------------------------------------------------------------------------------------------------------------------------------------------------------- Grebe, Jun[iacute]n Podiceps Peru Entire E NA NA taczanowskii -------------------------------------------------------------------------------------------------------------------------------------------------------- * * * * * * * -------------------------------------------------------------------------------------------------------------------------------------------------------- Plantcutter, Peruvian Phytotoma Peru Entire E NA NA raimondii -------------------------------------------------------------------------------------------------------------------------------------------------------- * * * * * * * -------------------------------------------------------------------------------------------------------------------------------------------------------- [[Page 649]] Rail, Jun[iacute]n Laterallus Peru Entire E NA NA tuerosi -------------------------------------------------------------------------------------------------------------------------------------------------------- * * * * * * * -------------------------------------------------------------------------------------------------------------------------------------------------------- Tit-spinetail, white-browed Leptasthenura Peru Entire E NA NA xenothorax -------------------------------------------------------------------------------------------------------------------------------------------------------- Tit-tyrant, ash-breasted Anairetes alpinus Bolivia, Peru Entire E NA NA -------------------------------------------------------------------------------------------------------------------------------------------------------- * * * * * * * -------------------------------------------------------------------------------------------------------------------------------------------------------- * * * * * Dated: December 16, 2009 Sam D. Hamilton, Director, U.S. Fish and Wildlife Service [FR Doc. E9-31102 Filed 1-4-10; 8:45 am] BILLING CODE 4310-55-S