The Chassahowitzka NWR is primarily accessible by boat and is located approximately 65 miles north of St. Petersburg, FL. A boat ramp, maintained by Citrus County, provides a launching point for boats on the Chassahowitzka River for a small fee. To get there take U.S. Hwy. 19 north from St. Petersburg (or south from Perry) to State Road 480. Proceed west on State Road 480, the Chassahowitzka Boat Ramp is located at the end of this road. The refuge begins approximately three miles down river from this boat ramp. 

The Salt Marsh Trails parking area is just off Mason Creek Rd. in Homosassa. From US 19 turn onto Yulee Drive and continue until you reach Mason Creek Rd on the left just across from the Homosassa Elementary School.