Holt Collier National Wildlife Refuge is located in the Darlove, Mississippi area, about 29 miles southeast of Greenville, Mississippi, in the heart of Mississippi's Delta Region. From Greenville take Highway 82 east to Highway 61. Turn south (right) and proceed on Highway 61 to Avon Darlove Road. Turn east (left) and proceed on Avon Darlove Road to Darlove, Mississippi.