From Key West- head east/north on HWY US 1 to Big Pine Key.  The Nature Center is located just past the only traffic light on the island, on the left (north) side of the road.  Look for the blue Florida Keys National Wildlife Refuges sign.

From South Florida and upper Florida Keys- Take HWY US 1 south and west to Big Pine Key.  The Nature Center is located just prior to the only traffic light on the island.  Turn right, (north) into the Nature Center parking lot.