There two Hunting Zones on the Trempealeau National Wildlife Refuge, the North Zone and the South Zone.
South Zone: On Refuge land south of the main channel of the Trempealeau River and south of State Highway 35/54, a Refuge Permit is required for all hunting activities. Additional special conditions apply for permitted hunting in the South Zone. Please refer to the information below for season dates, special conditions and application instructions for permitted hunting opportunities.
North Zone: On Refuge land north of the main channel of the Trempealeau River and north of State Highway 35/54, refuge land is open to public hunting and a Refuge Permit is not required for hunting activities.