The refuge headquarters is located at 316 Marsh Causeway, Knotts Island, North Carolina, 27950. The refuge office is one mile south of the Virginia state line on NC Route 615. 

From the north (or by road) - To get to the refuge directly by highway, follow Princess Anne Road 11 miles south of Pungo, VA (near Chesapeake, VA). 

From the south- take the Knotts Island Ferry (Free) from Currituck Courthouse on US Highway 168 and follow SR 615 north. The refuge office is located on SR 615 approximately 8.1 miles north.